Radioimmunoassay technique (RIA)

This lecture explains about radioimmunoassay techniques or RIA. Radioimmunoassay (RIA) test is a very important in vitro assay method used to measure concentrations of antigens (for illustration, hormone levels in blood) through use of antibodies. As such, it may be visible because the inverse of a radiobinding assay, which quantifies an antibody via use of corresponding antigens.
Watch this video lecture to understand the mechanism of Radioimmunoassay also known as RIA.
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Thank you sir. I work in a Regulatory Science division. I needed to understand RIAs and now I do.

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That was great!! Thank u
But what we studied was there are blocking agent which gets displaced & also the supernatent is discarded rather than detected for radioactivity!! Also conc. is inversely proportional to radioactivity. I think i m more confused now!!

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It was a wonderful explanation thank you.My question is ,,the more radioactivity means the more antigen present in patient serum and it is directly proportional to the patient Ag serum but in case of hormone is it the same ...? because i read somewhere that hormone in the serum sample inhibits binding of the radiolabbelled hormone thus more radiactivity =less hormone .I would appreciate if you can reply me back to explain it furter .Once again thank you ...you are awesome

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[@abdullahmarwat2714]

according to RIA when radiolabeled Ag combine with Ab ,the reaction will occur and it gives a signal like color graph or whatever so, but only free labeled Ag give no result. So concentration of Ag (in patient sample) is inversly propotional to the radioactivity.

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How do they displace....if they already attached then how would newly added patient serum ag will compete...

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