Long-term Culture Storage in Water. All you need to know to save your babies forever!
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- čas přidán 3. 07. 2024
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Thank you for going through the trouble of acting out the procedures just to teach the rest of us. Much appreciated.
6:10 Man, I'm so forgetful. I don't even remember the last time I forgot something.
Lol
Me too, It can be frustrating when it happens daily, and gets expensive ,but well pull through with some TLC
I bought some Sharpie Industrial markers last year and they've been so much better than the standard ones. They work even on slightly moist surfaces, work again after getting wet with a quick line on a sheet of paper to dry out, and while still run with alcohol exposure are much more resistant to it then the old type.
Whoa, being able to write on moist surfaces is huge! But how easy is it to erase with alcohol? I've noticed that it becomes difficult to wipe off anything after it's spent a couple hours in the PC -- somehow even dry erase markers seem to permeate into polypropylene after a stint of 250F heat.
Omg the tape over label is genius. Been using grafting tape for plates.
That is funny. Someone mentioned using Scotch tape to stop Sharpies from running with alcohol on spawn bags the other day. I thought I said the thing about tape in a video somewhere, but couldn't remember where. Thank you :)
Keep up the great work 👍 👏 💪
There he is...thanks for this little tip Ed!! i just started doing this with little glass 10 ml containers from the dollar tree. Now i can store lots of genetics in a small area👍
I'm always on the lookout for new containers. I think I've finally settled on the 50ml centrifuge vials and the 2ml cryovials. They are autoclavable and have nice hard threads on the lids. The cryovials are kind of tiny though. Really depends how much keistering I plan on doing ;)
Have a good night teacher and classmates ❤❤❤
Ed blessing us with the knowledge. 🍄❤️ Thank you, Sir. For all you do. ✌️
I solved my labeling problem by making labels before I do any mycology work. Therefore, I just attach it to whatever I’m using at the time after I’ve completed the task. The only issue now is remembering to also put the date on them too! Ha ha
Best regards from Detroit 🙂
I use a Dymo 450 with 3rd party labels. When making the "template" in the software, there's date insert that auto changes to the date of the day you open the app. It's great, clean and you can pump out the labels for the day you're working. There are also some hardcore 3rd party labels that are freeze and autoclave proof. If you go Dymo, DO NOT get the new 550. They went DRM with the labels.
@@JoeMushrumski - Excellent. Thanks for the tip🙂
The abbreviations drive me nuts. Talk about forgetting what the hell it is/was. And misplaced the logbook. hhahaha
I put it in my phone and send myself an email every few weeks with it all copied.
Dr. Grand, keepin' it real, as always.
Thanks from CT Ed!!! Appreciate the knowledge
Thanks Ed!
Thank you from Az
Thank you!
Especially enjoyed the inside humor (thinking you have something special extraordinary, thinking you’re goin to slow down…). Technical tips also spot on of course!
Thoughts on dehydrating colonized grain and vacuum sealing it as another long term storage option?
Thanks for the great information Ed!!
Thank you for sharing your knowledge! I've been worried about storing my cultures long term with slants I'm going to try this instead
Love your videos dude
Love the commentary and sense of humor. I love the ideas for cheap plastic wrap. Costco roll of plastic wrap= forever tape. Thanks, Ed!
My vote is amazing .. thank you brother. Best CZcams channel ever!!
Thank you very much. It warms my heart to know I'm helping people and you're enjoying the content.
Dear Ed, Where have you been my whole life? Another classic Baby Grand, Once you get the message hang up the damn telephone, right? I'm gonna be more deliberate with my labeling and resist the urge to swirl and shake. My animal instincts had already told me it needs to be put it away and just forget about it and resist the urge to agitate the mycelium at work and yet, I'm a effing savage. I'm gonna break these bad habits in your honor. Thanks again Ed, Goodnight🎃
If you put a link to Amazon for all these storage vessels, I would use the link. ✌🏻
The links change every day. It would be outdated immediately. Just search '50 ml centrifuge tubes'.
Hope your doing well edd mush love at yeah 🍄❤
How long do you pc these for ?
Do you suggest also keeping plates at room temp for storage also ?
Yes. I keep everything at RT. Well-wrapped, they will be good for 6 months at least.
Great information! You can store these both at room temperature and refrigerated?
I just keep them at RT. Saves space in the fridge. Some species apparently don't like the cold.
@edwardgrand Is there a source for this that establishes the longevity being claimed?
Yes. It is quite well-known. You can find it on the internet. Sorry, I have read it many times, but I'm not up for an internet search.
You can start here:
www.researchgate.net/publication/248729085_A_miniaturised_system_for_storage_of_fungal_cultures_in_water
Thanks for posting another super informative video! I haven't seen anybody else demonstrate water storage. Despite the increased simplicity and longevity, for some reason a lot of people seem to prefer traditional agar slants. You had me sold at...50,000+ samples stored at room temp :D
Btw do you mind sharing what the liquid you have your scalpels immersed in is? Is this a good substitute for using a sterilizer/alcohol burner/heat based technique?
70% ethanol.
@@edwardgrand Wow, in your experience just leaving the tools submerged in 70% ethanol is a good substitute for flame sterilizing? I always suspected that flame sterilizing is overkill for our application but have no proof; but my only evidence for the efficacy of ethanol is information published by the CDC.
@@vincentwu2848 I don't know if 70% is enough. I also flame sterilize. I always flame sterilize between transfers. The 70% alcohol jar is to store them and cool them down. After working for hours in the hood, the metal handles get quite hot. Hot blade in 70% is gonna kill anything.
@@edwardgrand Ahh okay. Thanks! I'm definitely going to have to copy your technique as it looks way too sensible to pass up on.
Btw, there is some literature about long term preservation of fungal culture on anhydrous silica gel -- I'm assuming the type of fungi we are dealing with can't be stored with this method? Just thought I'd run the idea by you, even though I'm definitely going to be copying your water storage techniques as well :D
@@vincentwu2848Lyophilization/freeze-drying techniques have been used with some fungi, but I'm not sure how well they work with filamentous fungi. I think most of those techniques are based on spore/conidia producing fungi. I've never explored it due to the cost of the machine and survival rates. I think the water technique might be best of it's ease, expense, and speed. Can you find me on FB, IG or Discord and send me the reference? I would love to discuss it more, but here it is hard. I don't think it'll let you post links here?
ive been getting older for years . if i threw away everything i can,t remember then i wouldn,t have much lol. i label but get confused bout the abbreviations . i have the green centrifuge tubes but i hate poring and im afraid of trying no poor with those things . i hate boil over , very frustrating .
Just PC tap water and throw normal agar pieces in there. Don't move it, keep it in a dark place. I love the sealable plastic containers/Tupperware. They hibernate. Good for many years. People have pulled live cultures from 20 year old water vials!
How long do you pressure cook it for? I couldn’t understand what you said! Love your videos!
1 hour. Let them cool overnight before opening the PC.
@@edwardgrand ok awesome then I’ll be able to keep them on the counter for years?
@@Houseofmycologyyes. Try not to disturb them, especially the surface pellicle.
@@edwardgrand I’m new to mycology what’s the surface pellical? I’m learning from your videos!
@@Houseofmycology The layer of mycelium that forms on the top of the liquid. Like a SCOBY for Kombucha. It will make the underlying liquid anaerobic, which is what you want to keep the mycelium in hibernation mode.
Do you sterilise the water with the tube before ?
Yes. Tap water. 1 hr at 12 psi. Lids 1/4 turn loose. Cover the whole rack with foil. PC. Cool. Tighten lids until use.
@@edwardgrand Thank you
@@edwardgrandfor the small tubes too?
@Sage_Advice cryovials yes. Eppendorfs no. They need a screw lid.
how long do you throw these in the pc for?
1 hour at full pressure and temp
@@edwardgrand the water needs a full hour? wild
@@undeadzomboy It doesn't 'need' it, but I'm kind of an overkill guy. I'd rather spend an hour now instead of pulling cultures in 5 years just to find out they are all contam'd. Makes more sense to me.
@@edwardgrand thats fair! thank you!
"EDDY, OH YEAH BROTHERRRR!" (In my macho man voice) !
"Long term storage is coming, AND YOU NEED TO TO LISTEN UP BROTHER!
“Best there is; past, present and future! Ohhh yeahh!”
"Sometimes your agar is like too hot to handle, and too cold to hold!”
“We’re in space, and space is the place!”
“Expect the unexpected in the (mycelium) kingdom of Madness!”
OH YEAH BROTHER, I CAN DIG IT! That damn Slim Jim commercial with Randy is embedded in my head forever. I'll try to do the voice on the next video if I can remember :)
I tried...
instagram.com/reel/CuOVifsxrAq/?igshid=MzRlODBiNWFlZA==
Very much appreciate the video bro!