How to interpret the results of proteomics data analysis? Proteomics Data interpretation

#howtointerpret #proteomics #interpretation
In this video, I have explained how we interpret the data proteomics from basic, and provided a practical guide on how the conclusion from figures and visualizations can be written well.
Review article: A practical guide to interpreting and generating bottom-up proteomics data visualizations
DOI: 10.1002/pmic.202100103
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[@armankulyyassov]

In fact, SILAC, TMT, and other methods use stable (non-radioactive ) isotopes with 13C and 15N en.wikipedia.org/wiki/Stable_isotope_labeling_by_amino_acids_in_cell_culture (5:17 min)

[@asifmolbio]

Sorry what i mean is the use of isotopes

[@aiswariyaramesh6998]

Sir, is it possible to use volcano plot for proteins obtained in a single set of control and treatment whole cell proteomics data

[@asifmolbio]

Its possible

[@teodoralukic24]

Thank you a lot for your videos! :) It would be nice if you can make an interpretation of RNA seq and ChIP data soon. :)

[@asifmolbio]

Thanks, Teodora, Please note RNA seq data interpretation videos are already uploaded on my channel.

[@asifmolbio]

How to calculate FC, log2FC, Pvalue, Padj, Up/down genes in RNA seq data using Excel
czcams.com/video/HH3Mll4W5WE/video.html
How to find genes of interest in RNA-seq / transcriptomic data ? Kegg pathway
czcams.com/video/LeNjskbF8RY/video.html
Interpretation of Principal component analysis (PCA) in RNA seq
czcams.com/video/aUOV7EBGOy8/video.html
How to select genes for qPCR validation in transcriptome/RNA seq data?
czcams.com/video/PRhHBcjKAKU/video.html
How to interpret the results of KEGG pathway analysis?
czcams.com/video/meTGfLVBiRs/video.html
How to write and interpret gene ontology (GO) in research articles ?
czcams.com/video/kRt2IWfhCqU/video.html
Interpretation of RNA-seq heat maps
czcams.com/video/cRT5mpGMDmQ/video.html
Interpretation of RNA seq data | DEGs | GO | KEGG
czcams.com/video/xc2zEI_5pmw/video.html
You can watch them in reverse order, last one first and watch one by one while moving up. Hope you will get answers.

[@meemmerani]

Nice 👍🏻

[@asifmolbio]

Glad you like it

[@amrghit]

Thank you so much for this helpful video. Please is there a book allow me to understand these figures and how to create them??

[@asifmolbio]

There is no book but review article you can find online

[@saboorahmad316]

Thank you. Sir any tutorial video about MxQuant software for phospho data?

[@asifmolbio]

Thanks saboor, i have noted down your suggestion. Please stay tuned.

[@ohikana4970]

Salam. Is there a certain website for proteomics data analysis? These figures are very nice but seems like they are using Phyton programming to generate them. Thank you

[@asifmolbio]

Yes there are websites, and these figures are generated through python. Their code are available (on Git Hub) and free to use.

[@ohikana4970]

@@asifmolbio Can you share the websites please? I know ShinyGo from you. I have no experience in Phyton

[@birrabukhari3836]

Sir, My question is how to analyse protein data? is there any platform like iDEP where we can analyse the proteomics data? Kindly guide me regarding that. Thank you.

[@asifmolbio]

Yes there are but its paid, but differential analysis can be done freely

[@birrabukhari3836]

@@asifmolbio Sir, Kindly make a video on how to analyze protein as well.

[@birrabukhari3836]

How to do differential analysis free for protein?

[@parthibanpana9905]

thank you for your wonderful videos,..sir, please do one video for metabolomics data

[@asifmolbio]

Soon inshaAllah , stay in touch

[@chandanmahata5241]

Thank you for the information. May I have your email id, please. I have several queries regarding proteomics analysis.

[@asifmolbio]

[e-mail]