Isolation of vesicles from human blood by Sepharose size exclusion chromatography

Standard operation procedure to isolate exosomes and other extracellular vesicles from human blood by Sepharose (CL-2B) size exclusion chromatography. Please cite as dx.doi.org/10.3402%2Fjev.v3.23430. This work was funded by the European Metrology Research Programme under the Joint Research Project HLT02 (metves.eu).

[@nnatywnaa]

Hi :) I'd like to know which type of prepacked Sepharose CL-2B columns do you use? I couldn't find any on the GE Healthcare website. Or perhaps do you have regular PD-10 column and pack it yourself with the Sepharose gel?

[@mr54vintage]

+nnatywnaa
Izon Science makes the qEV developed and standardised in association with AMC and MetVes.
www.izon.com/products/qev/

[@ivanrestrepo6788]

Hi, thanks for the video. I saw there are two filters (one at top and the other at the bottom) inside the syringe. What is the size of those filters? where can I get them?

[@kylemarra8363]

I'm curious about the same issue - what filters did you use for the top and bottom of your SEC column. Great video - thanks for the instruction!