SDS-PAGE (LIVE DEMO)

Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is commonly used to obtain high resolution separation of complex mixtures of proteins.
In SDS-PAGE, the use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel largely eliminates the influence of the structure and charge, and proteins are separated solely based on polypeptide chain length/Molecular weight.
Coomassie blue dyes are a family of dyes commonly used to stain proteins in SDS-PAGE gels. The gels are soaked in dye, and excess stain is then eluted with a solvent ("destaining"). This treatment allows the visualization of proteins as blue bands on a clear background.
SDS-PAGE is a reliable method for determining the molecular weight (MW) of an unknown protein, since the migration rate of a protein coated with SDS is inversely proportional to the logarithm of its MW.

[@zainpal268]

Very helpful video

[@glykeriarigana7195]

Why are you making poly acrylamide gel without gloves ????????????

[@diksha4942]

It's a very informative video

[@sabitasinku9548]

Very helpful vdo😁😁

[@nemkumarsahu8050]

Thank you sir ji 🙏🙏

[@thebiobuddy9595]

Thanku so much, you saved us

[@NirajSingh1979]

Happy to help

[@sabitasinku9548]

Tq

[@shubhisingh7157]

Please share the protocol for sample preparation.

[@vamshikiran1404]

Hi sir
Your video is very helpful
Please provide western blotting technique also sir

[@sachin7709]

I don't get gel

[@hirengoutam4796]

What is the total cost of equipments and from where we could buy?

[@NirajSingh1979]

It starts from approx 1.0 lakh... And you can purchase from any scientific vendor...

[@zahidbashir8946]

Can u please share the protocol of reagent prepration

[@NirajSingh1979]

Share your email

[@sangeethakumari9302]

Pls share protocol fr protein sample preparation from tissue sample

[@PankajKumar-md5cb]

I meet to you prosibal?

[@aashikas3859]

Sir, can you pls share your mail id

[@NirajSingh1979]

[e-mail]

[@aashikas3859]

Thank so much sir

[@aashikas3859]

​@@NirajSingh1979 sir, i have mailed pls once check