Helpful video, but NOT WEARING GLOVES WHEN HANDLING THE GEL MOST OF THE TIME! I'm not usually hung up on safety stuff, but in this case, liquid acrylamide is a neurotoxin, and exposure can lead to progressive peripheral neuropathy. You can never be sure that the gels are 100% polymerized. Kids - don’t do this stuff without a lab coat, safety goggles, and at least vinyl gloves.
I still don't understand why it's not allowed to have inner buffer leak to the outer buffer in this video. It's appropriate to overfill inner chamber and have inner buffer leak out in my lab and so is some protocols.
you are allowed that is to say, it'll work, im not sure if ALWAYS, but in the youtube videos put out by the bio rad manufacturers the electrophoresis demonstration involves overflow, I'm not sure if it was SDS though
You need to maintain constant flooding of the fluid on the gel, the fluid actually has different pH gradients from the outside buffer. If you have a leak you risk equilibriating the volumes and getting a dry gel, this can mess up the results. But even if it isnt a full seal it will still work as long as the gel remains flooded with buffer.
A very explanatory step-wise tutor. Thanks so much
So nicely explained, I give this to my project students before they enter the lab :) !
Thanks for assisting with my crisis after lab members went home :D
Great video! Thanks for sharing!
I felt like I was listening to my gearhead garage dad explain how to change the oil XD
Helpful video, but NOT WEARING GLOVES WHEN HANDLING THE GEL MOST OF THE TIME!
I'm not usually hung up on safety stuff, but in this case, liquid acrylamide is a neurotoxin, and exposure can lead to progressive peripheral neuropathy. You can never be sure that the gels are 100% polymerized.
Kids - don’t do this stuff without a lab coat, safety goggles, and at least vinyl gloves.
Great video! Very insightful.
Thank you for giving us the video version of detailed steps much better than reading texts that is so difficult to understand.
Thank you! This is extremely helpful
Thanks for this great video!
Comprehensive and clear explanation. Thank you
LOL @ 7:21
oh gosh, love it when that happens
Thanks for this video........keep it up.......
i have a doubt . should the plates go in the space behind the ears or should they sit above the ears in the electrode assembly unit.
what is the advantage of choosing constant voltage over constant current?
Thanks for upload this useful video
What a constructive presentation
awesome.. one of the best
Wonderful Video
Helpful indeed!
Very professional explanation
Thank you for the video! It was really helpful :)
You know what this is the latest advanced equipment that we have bought in my university
Тhank you!
Talk about precision.... ;-)
Thanks sir
awesome
There are 3 types of SDS PAGE, What's the difference between the 3 types?
1. Native SDS PAGE
2. Reducing SDS PAGE
3. Nonreducing SDS PAGE
IS NOT A TEST SIR
its good
I still don't understand why it's not allowed to have inner buffer leak to the outer buffer in this video. It's appropriate to overfill inner chamber and have inner buffer leak out in my lab and so is some protocols.
you are allowed that is to say, it'll work, im not sure if ALWAYS, but in the youtube videos put out by the bio rad manufacturers the electrophoresis demonstration involves overflow, I'm not sure if it was SDS though
You need to maintain constant flooding of the fluid on the gel, the fluid actually has different pH gradients from the outside buffer. If you have a leak you risk equilibriating the volumes and getting a dry gel, this can mess up the results. But even if it isnt a full seal it will still work as long as the gel remains flooded with buffer.
08:13 That's a good load. Lol
he didnt don gloves
9:00 Don't panic,reload about 1mm between the space
Of well
WHEN DID YOU ADD THE GEL?
He used a gel that its ready to go.
Nice instruction - but, why are you not wearing gloves!!
This is mine project
vietsub,please!
hhhhhm neurotoxins... wear your gloves kiddos