Great video, super well explained! This method looks wayy easier than the liquid culture one, which takes lots of effort and material. That'd be possible to only reproduce mushroom cultures in agar? I've tried to grow some Shiitake here in Rio, with corn, but it started growing a black mould.
Thank you for making this video! I want to grow basic white button mushrooms for home use and I can't seem to find any grain spawn or liquid culture for them. I guess bc they're not gourmet or exciting. Now I can make my own!
@@SporenSprout I went to your Etsy listing and under the "Select an Option" menu, I didn't see any sort of button mushroom available. But I did see it in the pictures?
Hey brotha. So I just made my first agar plates with shiitake mushrooms from the store. Followed all of your steps. How do I store these? Room temp? Or in the fridge while the mycelium grows?
If it doesn’t rip you can wipe it down with isopropyl and dry to reuse it for the life of that individual Petri dish. You can use your discretion. I think that grafting tape holds up a little better than parafilm does.
70 percent works best because it has a mix of water in it which evaporates quickly. I wouldn't try to substitute it with ethanol unless you dilute it to make it 70 percent.
This is just what I've seen in videos, I've yet to do it but: you should be able to cut a small section from the colonized agar plate and put it into some sterilized grain, inoculating it and making it grain spawn which can then be mixed with substrate and grown in various ways.
note: make sure it's agar to agar, if you sandwich the mycelium you'll risk slowing growth if not suffocate it completely.
bro your videos are so enjoyable to watch, smooth recordings and such a nice edit, keep the great work
Thank you so much! 🙏
Great info
Great video as always, thanks for putting all these together - they’re very informative and helpful for beginners!
Do the agar plates need light or darkness to grow?
I have not noticed any difference between one or the other.
Quick question after the transfer do you put straight in the fridge or wait till after the the mycelium growth for storage ?
Great video, super well explained! This method looks wayy easier than the liquid culture one, which takes lots of effort and material. That'd be possible to only reproduce mushroom cultures in agar? I've tried to grow some Shiitake here in Rio, with corn, but it started growing a black mould.
Can u do this with turkey tail also ?
Yes
Thank you for making this video! I want to grow basic white button mushrooms for home use and I can't seem to find any grain spawn or liquid culture for them. I guess bc they're not gourmet or exciting. Now I can make my own!
I have some
@@SporenSprout I went to your Etsy listing and under the "Select an Option" menu, I didn't see any sort of button mushroom available. But I did see it in the pictures?
@@Batsnang I will be adding it back on today
Got a new sub.
much appreciated brother!
Great demo
Thanks! Here’s the updated video!
czcams.com/video/EARNzNHAzf0/video.html
Nobody tells whats next after agar production
Here you go:
czcams.com/video/EARNzNHAzf0/video.html
What kind of scalpel is that and where can I buy one?
I recommend these: amzn.to/3FS2Gdf
Thank you for this video. Very much appreciated
Thank you for watching!
do i need to seal it with that tape
Yes, parafilm or grafting tape works. Check out the more recent tutorial:
czcams.com/video/EARNzNHAzf0/video.html
Hey brotha. So I just made my first agar plates with shiitake mushrooms from the store. Followed all of your steps. How do I store these? Room temp? Or in the fridge while the mycelium grows?
Room temp while it’s growing and refrigerate once you are satisfied with amount of growth.
@@SporenSprout thank ya kind sir. Your reliability is unparalleled.
I noticed you reused the parafilm on the petri dish. How many times can you reuse the parafilm?
If it doesn’t rip you can wipe it down with isopropyl and dry to reuse it for the life of that individual Petri dish. You can use your discretion. I think that grafting tape holds up a little better than parafilm does.
Where do you store them, and how long are they good for?
Store them on a shelf at room temperature for around 6 months. They are good until the start drying up.
Will ethanol be a fine substitute for the alcohol?
70 percent works best because it has a mix of water in it which evaporates quickly. I wouldn't try to substitute it with ethanol unless you dilute it to make it 70 percent.
nice information...
Any advice for cultivating blue meanies from spores?
Yea. Watch my video on how to make BRF jars or my video of how to make grain spawn and then inoculate with your syringe.
@@SporenSprout thank you beyond words. I appreciate you
Thank you sir👍🏼
Okay, but what are the following steps after this, if you want to grow the mushroom fully?
This is just what I've seen in videos, I've yet to do it but: you should be able to cut a small section from the colonized agar plate and put it into some sterilized grain, inoculating it and making it grain spawn which can then be mixed with substrate and grown in various ways.
You have a few options from agar, you can add the agar directly to your grain jar/bag, make a liquid culture, or continue isolation genetics.