From Beer Cooler to a DIY Incubator
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- čas přidán 7. 07. 2024
- Two of the most useful tools in biology are also some of the simplest and are just things that need to warm up to a particular temperature and stay there. Today we're building two such devices, an incubator, and a heat block.
Incubators keep their contents at a nice even temperature to speed up the growth of organisms. Heat blocks are dense, dry, heat sources for heating reactions to specific temperatures, often far higher than an incubator will ever go. Heat blocks are used during ligations, transformations, restriction digests and more. Best of all heat blocks are temperature regulated so there's no need to babysit them the way you do with a water bath.
Probably the best part of this design is that it uses such readily available materials that it can be adapted for whatever vaguely warm container applications you'll have, like building bioreactors, which we'll explore in future videos.
Materials:
Peltier - www.amazon.ca/ZTDM-TEC1-12706...
Temp controller 1 - www.amazon.ca/gp/product/B01E...
Temp controller 2 - www.amazon.ca/gp/product/B01I...
Heating pad - www.amazon.ca/gp/product/B018...
Follow up video:
Meatcubator - • This Machine Grows Liv...
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_____________________________________________________ - Věda a technologie
Hi All. I know I shouldn't be using an IR thermometer on a shiny surface. That was just to see if it was heating up. I used a glass thermometer and calibrated thermocouple off camera to confirm this was working the way it was supposed to. When that shot was recorded I was contemplating putting paint on a spot so I could also measure with the IR.
And I know I should have added a heat sink to the other side of the peltier, but doing so would mean faster heat dissipation when the peltier is off, so while less efficient it does trap heat better this way.
Speaking of things you shouldn't do, you shouldn't have made this video without trying to make it a collaboration with uncle bumblefuck, aka AVE. You are still looking for Canadians to do a collaboration with right?
electrical tape (vinyl) is perfect for things like this. It has the correct emissivity for most ir thermometers (~.95), and is close enough for thermometers adjusted to an emissivity of 1.
Panel-mount pid controllers can be had for a song on ebay and the like. These are great, because they really open up your options if you want to expand or change the project. If you're REALLY handy, you can of course build something from the ground-up using an arduino.
Remarkable. :) To think that an individual, not a government or commercial entity, but a private citizen has access to the information and resources necessary to reproduce in the comfort of their own home some of the same instruments and capabilities used in modern, professional scientific, engineering, and industrial fields... Love it. What an age to live in. I hope videos like these inspire a new generation of creative thinkers and problem solvers to make full use of the liberties and resources available to them.
It used to be the same for computers. Over time we get Major Governments -> third-world governments -> Major corporations -> Wealthy individuals -> Holiday day-trippers. We are seeing the same progression in space travel now.
The infrared thermometer isn't great to measure metals. Due to their high reflectivity in the IR spectrum. The thermocouple is probably more accurate.
I know, that's just what I had on hand while filming. It was also tested with a glass thermometer and a separate thermocouple.
"Warm version of a fridge" got me laughing
Loving this whole damn series
*heating block set to 42.0°*
Ohh right, he's from Canada!
He's from science too, so there's that.
@@SianaGearz Where's science located at?
@@MishoIV science generally uses the metric system
Maybe use thermal paste in between the Peltier and the block you will get much more efficient transfer of energy
honestly one of my fav channels with cody’slab
Dear friend I greatly like and appreciate your hands on approach to scientific projects. I will greatly appreciate a tour of your lab in some future video. Good luck.
You've made an instant fan of me.
Those SPARCstations look so rad
Man I bet it'd be cool AF to come hang out with you for a week and just make stuff and do science 😆. This is pretty sweet! 👍
When I was looking at both Brie making or poultry egg hatching, people seemed to really like grabbing smaller broken wine coolers, or smaller refrigerators for like cola, or Energy drinks that get given away on craigslist for making their own incubators. Might be nice to add it to a watch list if you every want to scale up.
When this showed up as a search result, I was wondering who could make such a concise, well-made guide for such a thing, but then I saw that it was Thought Emporium.
Hey I used a PCR machine in my biomedical engineering unit just the other week!
Paltier devices create a temperature differential between one side and the other which is only really useful for cooling, any reason why you went with such a device over resistive heating?
Also you might want a sink for the cold side, as it stands your insulating both the hot and the cold side of the paltier in the same box
Do you have any comparison on heat efficiency? I'm curious now
@@0Arcoverde I'm not sure, it's abit weird because I've only ever seen them used for cooling.
They create a temperature differential, but in the process generate their own waste heat, so keeping them in an insulated container will eventually heat the container. But any electrical device will do that
On the other hand if you have a method of conducting in heat from the outside world to the cold side rather than insulating it, the device will take said heat and pump it to the hot side.
So for their efficiency as a heater , it depends on how you use it.
A good way of thinking of it is keeping your fridge open to heat up your house, fridges use energy to produce a temperature differential, but also generate waste heat that is essentially equal to the amount of power they run off of.
@@0Arcoverde I think the heat pump method he is using works better than resistive heating, however the more you insulate both sides together the closer it gets to the efficiency of just using a resistive heater
I'm guessing he grabbed whatever was good enough. Besides, Peltiers are easily repurposed, and he plans to do PCR soon.
@@WilliamDye-willdye yea I mainly brought it up as a way to get more heat out of the heat block without increasing the wattage, by putting having a sink for the cold side that can get heated by the surroundings
I remember using PCR and incubator in my university.
Is using it thermometer on aluminium (low emissivity) giving You correct readings? Why did You leave such long screws in this block? Wouldn't it be a bit more esthetic to cut them shorter?
Anyhow, great video! Congrats, on developments on new lab!
no, It will be out. Should paint the top matt black
Yeah, you need that mill working. Hackerspaces need a mill!
Your temperature readings (e.g
at 6:43) will be rather inaccurate because of the reflective surface of the aluminum. For our heat blocks we tried adding a matte black surface for a while, but eventually gave up and measured temperatures with a separate probe in each well.
I can suggest to use Kapton tape (the yellow clear tape people use for 3D printing). It has a very well defined emissivity.
Hey First, I love your videos I can't find any other youtuber like you in my list you are the biologist along with a chemist and a electrical engineer. Second, I hope you can do more with bio Hacking I'm still having trouble understanding.
I was just wondering isn't it kind of counterproductive to have the peltier device inside an insulating wooden block when it would work a lot better with a heatsink on the other side as well.
Brilliant. Do you come up with your designs mainly by yourself? Or do you try to optimize ideas you find online?
5:25 you can always glue the pieces together with silicone
How do you calibrate your thermometers? I was using fever-thermometers, those who take a while to measure, but it's hard to get the same conditions for both things and they only work in a short range.
this is cool & I like all your content, but circling back to your RF farewell vid, are you done with radio experiments?
No but it's already starting to snow up here in canada which is getting in the way of stuff I had planned. A lot will have to wait till spring to pick up again. I meant to test the corner reflector this week but it's been raining and snowing which would screw with the antenna and also I don't feel like standing in the rain at 2 degrees for the sake of a video. I've got some stuff that can be done indoors planned but I'll get to it when I get to it. I'll need breaks from bio from time to time so I'll do radio and physics stuff then. Then come spring I'll do another big radio project. I've got stuff in the works but anyone who's followed this channel knows that I tend to pick up projects and put them down for a while as resources come and go. I haven't touched the styrofoam worms or plastic fungi for years but both just got restarted. Things are on a cycle. So please bear with me and be patient.
you should try to make a plasma gasification unit.
I have a more use for a flat heat block, that makes this a lot easier
I was waiting for you to talk about the twofer only to realise that you meant 2-fer. Derp
Heat shock in the heat block!
Could an old AMD CPU be used as a heater instead? It seems like it would work.
Note; You can buy coolers with little holes on the inside for draining melted ice, if you find one of those you don't even need to drill anything.
Nice project, really like where it is going, one thing that I didn't understand, why use the heat pad in the incubator on the roof? Hot air will stay in the top and the colder air will stay or sink to the bottom of the incubator, I thought it would be better on the floor of it, using an stand to put things without touching the pad, it would make it more efficient. Cheers
The heat pad is being controlled by being fully turned on and off. The pad itself can get way too hot so if it was underneath the plates it'd cook the organisms. The point of it being an insulated box is that the temp evens out. Better to let the heat percolate down slowly.
@@thethoughtemporium Oh, got it, thanks!
what about the cold side of the peltier?
the back side gets cold and doesn´t effect the system?
Isn't it necessary to use some thermal paste on the heatblock and temperature sensor? Or is it accurate/precise enough as it is?
It's probably fine, it isn't a CPU after all.
I'm not concerned about top temperatures, but OP wants the temp to rise faster and spread better in the heat block, thermal paste makes a HUGE difference to that.
@@bakedbillybacon That is true and it does help but it may be overdoing it.
so you are guilding a diy pcr machene (so am i) how are you doing the heating and cooling do you use a block or suspend the tubes in wather thst cycles?
What do you chisel aluminium with? Are there special tools for that? (Regular chisels would go dull, right?)
Normal chisel then sharpen it after. Aluminum is really quite soft.
If you are gonne make a bio reactor. We made our with a large diameter pvc tube and covered the bottem.
Gonna use a large fermenting jug or other glassware if I do.
I am a biochemestry student from belgium. i have shown your channel especially your videos of the crisper cas kit to my teachers.they where intrested in what you do and want to try the kit as well. If successful, they will follow your upcoming videos in making the spidersilk in yeast.
So good luck we will be watching and following you 😉
Ps:we have a pretty advanced lab so it will prob work
PS : L'alcool est un solvent pour l'encre de Sharpie.
(Alcohol is a solvent for Sharpie ink)
Im surprised that peltier works. Usually you would want some kind of heat break between the hot and cold sides of the peltier. I guess since the thermal requirement is low enough it still works.
Ya heat sinks are more important if you want the cold functionality. To just get hot is easier.
@@thethoughtemporium heat sink yes but a heat break is kind of important both ways. The way yours is setup you're drawing heat from the same chamber you're trying.to heat. It works because the chamber isn't that efficient but it's kind of energy wasteful:)
Yot! Do the pcr!
mathematicians have....blackboards! xD
Why not just use a thermocycler to quickly and accurately cycle between your two temp?
Hi
Could you try to make a proton exchange membrane (pem) for converting H and O into water and electricity? Without buying a ready-made one 😂
Can a heatblock be used instead of a PCR machine?
Anyone know how to add a shaking component ??
making one for queen bee rearing
I thought there were restrictions on incubating cultures at more than 25C in the UK?
I'm not in the uk? But that sounds backwards
Maybe it was just schools then? Just that the biology teacher said they weren't allowed above 25C.
Wasn't trying to poke holes in you; it was an open question really. Love what you do
I feel bad for those chisels
That does not look like a thermocouple, I would say NTC or PT100, but I could be wrong ;)
A great source of heaters are PTC heaters from ebay ... used some to make heated dry bath (czcams.com/video/bvRVtqmVO2A/video.html) and water bath (czcams.com/video/S1iwhdZjOuM/video.html)
what's up with those extra long screws? :D
Hand holds for picking it up if I ever wanted to while it was hot. Otherwise just trim them to size.
What about repurposing an old fridge as an incubator?
Works just the same as the version in the video, just use a bigger heat source. Couple IR lamps could work for that.
The heating pad out of a water mattress has worked great as a seedbox warmer for me. Would be great for this application.
3:25 it's the funny number
yay zero dislikes
couple of dislike from dead organism
you know that using a thermal meshuuring device wirtth a beam of light is not going to work verry well on reflective surfaves
Yes. It was just the first thing I had on hand while filming. It's been tested with other probes and a glass thermometer
well you can avoid the reflection isue when you just put on some electrical or painters tape
This heater block is jank af. ^^
Can you freeze a rat or something else cryogenically and then try to bring him back to life?!
No. The formation of ice crystals would puncture the cell membranes, permanently destroying the rats cells and killing it.
Great video. Very professionally explained. Is there any need for a heat block if you own a PCR machine?
Also I built a nice small Incubator out of a broken microwave and detailed it at specyal.com if anybody is interested.
A bit of a shame to (re-) use such a nice big heat sink, for only two little blocks...
It'll be used for tons of projects moving forward and we've got 2 of them.
IR thermometer on shiny metal = instant loss of respect in technical circles.
420 degrees...
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You are noticed.
senpai
hhhhhahah
you talk fast ,i miss a lot