Southern Blotting | Basic Procedure | In Hindi

#Southern #Blotting #BaaYo
Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA fragments by gel electrophoresis and identified by labelled probe hybridization.Basically, the DNA fragments are separated on the basis of size during electrophoresis. Separated DNA fragments after transferring on nylon membrane, the desired DNA is detected using specific DNA probe that is complementary to the desired DNA.A hybridization probe is a short (100-500bp), single stranded DNA. The probes are labeled with a marker so that they can be detected after hybridization.
Procedure/ Steps
1. Restriction digest: by RE enzyme and amplification by PCR
2. Gel electrophoresis
3. Denaturation: Treating with NaOH
4. Blotting
5. Baking and Blocking with casein in BSA
6. Hybridization using labelled probes
7. Visualization by autoradiogram
SHARE, SUPPORT and SUBSCRIBE!
Subscribe: www.youtube.co...
👋 😃 Connect with me on
LinkedIn 👉🏼 - / artirani
Instagram 👉🏼- / baayo_official
Telegram 👉🏼- t.me/baayo_cha...
Related Videos:
Western Blotting: • Western blotting techn...
SDS PAGE | Part 1 - Gel Preparation | Hindi : • SDS PAGE | Part 1 - G...
SDS PAGE | Part 2 - Principle | Hindi : • SDS PAGE | Part 2 - P...
Gel Electrophoresis | Agarose Gel Electrophoresis Lab Procedure : • Gel Electrophoresis | ...

[@BaaYo]

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

[@rishuyadav9667]

I have hardly seen teachers with such conceptual clearity and confidence like you. Warna aajkal to sab bs book se as it is copy paste krte h blindly.
Also use of regional language is appreciated.
👍 Commendable.

[@pronoobvines6178]

Really 1st time in my life I found a madam with lots of concept and basic knowledge 😁😁😁. Sorry if I hurt anyone

[@BaaYo]

Thank you MR.

[@Salmankhan-dx1do]

🤫

[@anitathakur4514]

Same here mam nhi hota to pta ni ky hota

[@zeeshantayyabi1392]

A big big big thankkkk u ma'am.......u have helped out me with great difficulty in this topic.......hatsoff to ur explaination🙌🙌

[@dilipsangam2091]

Wonderful

[@dilipsangam2091]

Wonderful Presentation.

[@parnikavishe3761]

Hello am I am a student of SY biotechnology and I regularly watch your videos and they prove very helpful to me especially during exam times. Thank you so much for such amazing lectures, keep making these videos they are really helpful 😊

[@anamikabhardwaj5170]

Seriously last moment pe video dekh Rahi aapka. And it's awesome... thanks alott...ekdum crystal clear concept...😊😊

[@BaaYo]

Thank you

[@kashifahmad287]

thank you mam so muchhhhh from Pakistan. now my presentation is ready for tomarow.

[@oishe3988]

Tomorrow

[@Dse629]

Very helpful mam. Aap bhut accha samjhate ho thank you mam.

[@rimjhimBedi]

Thank God I've got to watch your vids. Otherwise I would have skipped these blotting related questions in exams. Thanks to you and ur amazing teaching skills that made me secure good marks.

[@kanikamalhotra7220]

Thanku so much mam graduation students needs teacher like you........thanku soo much this is very helpful video ❤

[@BaaYo]

Thanks a lot

[@ph100k3]

Thank you mam...For Very Easy Way teach us❤💯🙌...Huge thanks to you and your team😍😇

[@BaaYo]

Thanks a lot 😊

[@user-uf2xk2ts2b]

Woow no words 💯💯 sidha screen se dimag ke andr

[@ammarsayal]

Madam hmesha se ap ke videos se bht help mili hn khas tor pr youtube pe jo itne fazul lectures hn un me ap ke videos ksi heere se km nhi
Thank you soo much👍❤

[@takini4918]

Bdi aasani se concept clear ho jate hai aapki videos dekh kr. Dhanywad

[@muhammadosama1874]

👏👏👏👏👏👏👏..u deserve best teacher award ....teacher of the CZcams award

[@maheklakkad9564]

Very clear concept , easy to understand, amazing way of teaching, mam thank you

[@BaaYo]

Thank you. Please share and subscribe

[@sreemotichatterjee4345]

simply explained such a big concept. Thanks a lot ma'am for making the concept literally crystal clear.

[@Euphoria321]

Love the way you deliver lecture❤ simple, elegant, full of basics❤ thankyou Madam!

[@jyotsnasrivastava7661]

Gjb mam scchi me aap mst batai pta h kb se pareshan the isko lekr .......... Hai aaj chain aaaya .😊😊😊😊

[@nandunandini6722]

Wow I like it aap ka lecture very easily se samajh aa jata hai thanks for you mam

[@rajyagurukrupa7398]

Thank you so much for this explanation....you are truly amazing ma'am ❤️❤️ your short videos are much better than other 1 hour lactures...

[@subhrajeetdash6226]

Thank you mam..I am from chemical engineering and I find it useful in my work too

[@crazyjanis8258]

No one understood us like u,marvelous explanation😘😍

[@ubaidullah8874]

Love you from Pakistan
Outstanding lecture
May u live long

[@priyanshipatel2484]

Really a great explanation, it helped me a lot, thank you mam

[@noumansaeed7853]

Very interested and beneficial lecture about southern blotting
Thanks such a great work

[@Sameerali-uw1tl]

Thank you mam 😊 apke pass ye quality hai less time me apne sab kuch bta deya awesome 😎 mam

[@languageofplants1031]

Mam ap bht axha smjati hain thank you soo much

[@sumitpatel868]

Daimmmmmnn , thank you so much 🥺🥺🥺
I was so worried about genomics, you explain so well and logically ♥️🥺

[@haleemasadia852]

Thanks yeh thanks Dil ki gehrayon se

[@vikrambiradar6411]

Love you mam ...
You saved my biotech exam paper from back log

[@deekshasharma7725]

Thank you ma'am 🙏🏻...... Mere all doubt clear ho gye apki video se😊

[@PoojaSharma-qt4rq]

You are such a great ma'am

[@simransadhwani5176]

Wonderful explanation mam... really love it my concept is fully clear now

[@ubaidshaikh2153]

I just see your Vedios and write confidently in Exam. Thank you and God bless you!

[@BaaYo]

Thank you

[@riyarajput4117]

Thanks from my heart . Mam you explain it in a very easy way thank you so much h❤

[@jawadjoya3004]

Well informed and have concept after almost two years thank you mam

[@Pharmacy891]

Mam very very thanks from my heart .for understood in easy of this difficult topic

[@aniketdas7803]

Very helpful, thank you mam ❤

[@babaralimalik1868]

Best way of teaching....

[@official.rajarshidutta]

Mam, I am so glad to see your channel grow. I hope you remember me. I have been your subscriber when this channel had hundred subscribers. I pray this channel hits a million.

[@BaaYo]

Thank you Rajarshi :)

[@sanju-bt8ss]

Thank you so much mam

[@husnainaziz8623]

Bravo, never learned these topics with such clarity. U seems a born teacher mam🤗

[@sciencetech2536]

You so beautiful 😍apan ka dill. Gya ha ... chemistry ke sare notes yhi se dekhta hu

[@Maitri24]

Thank You so much dear Mam becouse It's help lot mam your teaching and explation style is simply superb hat's of you mam please keep uploading new vedio it's help lot 👍👍👍👍👍🙏🙏🙏 a bigggggg thank You dear mam

[@junaidaziz6933]

I am student of BS Microbiology And Molecular Genetics 🧬 😅 from University of Punjab Lahore Lahore. CZcams is great teacher and this one mam too

[@fh19293]

Thanx a lot for clearing this topic in such a beautifully explained manner😁😁😁...just a request, can u plz explain the topic of 'autoradiography'

[@knowmore2800]

thank u mam
u made me understand the concept easily and clearly.

[@proudtobeveterinarian6717]

Mam 😊 now I became huge fan of you ...

[@satyabratatripathy1613]

It's amazing mam.. Really it is very useful for graduation students... Ty so much mam for clearing the concepts of us

[@ektasharma4816]

Very good explanation mam 👌👌👌

[@kartikghode4850]

Aap mere college ki biotechnology ki teacher hoti naa to aapke subject mai top krta tha😘😘😘

[@shreyaskulkarni5312]

Only bcoz of this video i subscribed ur channel 🔥🔥
Keep it up.... Superr🔥🔥

[@sultanmehmood5022]

Thanx a lot, mam its so easy way explained, shall never b forgotten.
I shall search for other blotting also

[@aishwaryawadkar2240]

U r amazing.. The way U teach this biotech concepts is hilarious... Thankssssss alot mam.. U r best teacher 🙏🙏

[@abhishekbbetageri6027]

Huh? What is hilarious in that??
Ma'am is not cracking jokes.
Btw, juz check the meaning of hilarious.

[@chintakayalpurnima1741]

Hello ma'am. I love all your videos. I am myself a Faculty in University, but I love the way u explain. Beautiful

[@syedmedicose7477]

excellent maam.... stay blessed

[@Chillrelax630]

Ma'am boht Acha teach krti hn ap♡

[@umeshrai9323]

Very nice explanation. I appreciate your lacture. .now i feel easy on the topic southern bloating

[@ruchikumari1865]

This video are very help for me... Thanks mam 👍

[@researchease]

Thanks and love from China. Simple and easy way me best explanation.

[@sanjibbaishya4468]

Such a cool personality... Learning made easy.

[@simmianam7721]

Awesome explanation... thank you so much ❤

[@NavjotKaur-sf9ie]

very nicely explained...Keep it up👍👏

[@nanavatijayesh8143]

Your teaching technique is wonderful and makes direct digestible by mind

[@user-mr5yt5ls1i]

Thank you ma'am excellent 👍 teaching

[@abeerfatima2704]

Simply outstanding

[@shikamarunara2827]

Loved ur explanation ma'am.. thanks a lot

[@DebanganaGupta-us7kp]

You explain this basic concepts very well. Thank you ❤

[@Rjt23]

Really mam ur a good explainer....

[@nidhi878]

Thank you so much it is really very much helpful

[@zaminali4937]

Amazing teaching skill

[@pranayapatil7264]

Crystal cleared!! Thanks❤ mam

[@BaaYo]

Most welcome 😊

[@pankajsaklani2343]

I am eagerly waiting for every video of you and it proves to be very helpful for me, god bless u

[@PraveenKumar-bv2xe]

Thank you mam for making this topic very simple and easy.

[@Professorf11]

Good job ... bhot achi samaj ati hai mam...
Me Danish from Pakistan

[@VetAnilturkiya]

Ma'am I love your teaching style help full conceptual

[@maheshswami5226]

Awesome teaching, awesome, outstanding, magnificent lecture by mol biology queen 👑👑👑👑👑, thank you so much ma'am 💞🙏

[@BaaYo]

Haha thank u so much

[@easywaytolearn5782]

It's a amazing explanation lot of ty ma'am . ❤❤❤❤

[@BaaYo]

Thanks for liking

[@kashwiagrawal9785]

A very good and clear explanation, your video helped me a lot...thank u so much

[@BTSARMY-sb8sh]

Thanks a ton Ma'm
I needed u
U helped me so much for the preparation of my presentation.
Can never thank you enough ever!!
Lots of Love & Respect from Pakistan 💜💜

[@siddhanathkagwade7411]

Ap log padthe bhi ho??

[@rajarshisen7543]

Mam your explanation is superb and very clear. All your videos are too good mam. I am a very big fan of your teaching.... Thank you so much mam🙏🏻🙏🏻.

[@shahbaloch4814]

The amazing excellent teaching method easly understanding style it is very helpful thanks A lot madom 😍

[@twinkle1548]

Wow!! Ma'am very nice explanation, literally have no words to explain.
Big fat thank you!!!!💕

[@parvinbegumaaraa2289]

Mam, thanks a lot, I have little bit doubt, but get clarified after saw this video, one kind request, can U do the same in English for better understanding to all. Thank you mam. Nice explanation.

[@muhammadnazakat9416]

Thank you mam nice to meet you.

[@kyns3946]

Million thanks ma'am, you are too good, and made this easy to understand.

[@Greensignal2022]

lecturer and lecture both r awesome..hamara dhyan kidar gayahi nahi .video sey bahey😁

[@honeypatel1860]

Really a great explaintion, thank you mam 🤗

[@abhinavparmar7777]

Currently I am doing MBBS and this video helped me lot in my Biochemistry subject...
Thank you 😊

[@Prince_sen_0001]

Best teacher ☺️ thanks dii🎉

[@toma902]

Ma'am,
You are great...

[@BaaYo]

Thanks a lot

[@crazycoins8923]

Hats off mam👍🏻👍🏻👍🏻👍🏻thank you so much.

[@jaydeepdumadiya2464]

Great ma'am...
God bless you
🙏🙏🙏🙏🙏🙏🙏🙏

[@Vishakram0]

Excellent

[@shazmanimushtaq8359]

Very nice explanation

[@MrZurghamak]

Keep helping students like this
A huge fan of ur skill😍

[@SiblingsArtZone]

thank you so much mam.. very helpful video

[@prachijain8031]

Very good explanation 👏👏👏👏

[@youngcartoon7196]

The way u explain it's very very very nice maam i m ur huge fan 🥰🥰

[@mahmudulhasan-ws5jw]

Thank you for clearing in such easy way