Western blotting technique | Principle | Step by Step | In Hindi
Vložit
- čas přidán 5. 09. 2024
- This lecture explains about the western blotting technique step by step in Hindi.
The Western blot is meant for detecting proteins. The proteins are first purified from cells and then separated. During the separation you need to choose whether to keep the proteins natural or denature them. Denatured proteins are often easier to separate, but you might be looking for morphological variations. Separation is usually done on a gel followed by electrophoresis.
Often the gel will be stained after the electrophoresis so the proeins are visible. This is done to make sure that the electrophoresis worked.
Then the proteins are blotted from the gel and over to a membrane, usually nitrocellulose, Immediately after the blotting the membrane are blocked so no other proteins will attach themselves to the membrane.
After the blotting the membrane is induced so the proteins can be detected. Usually specific antibodies for the exact protein you are working with will be used.
A secondary antibody that can bind to the first antibody is then added and the secondary antibody has some kind of detection mechanism, either fluorescence, enzyme or color.
Please watch this video to have a clear idea about 2D gel electrophoresis.
#BaaYo
Please Subscribe: www.youtube.co...
Related Videos:
SDS PAGE | Part 1 - Gel Preparation | Hindi : • SDS PAGE | Part 1 - G...
SDS PAGE | Part 2 - Principle | Hindi : • SDS PAGE | Part 2 - P...
Gel Electrophoresis | Agarose Gel Electrophoresis Lab Procedure : • Gel Electrophoresis | ...
#BaaYo #WesternBlotting #Protein
To access notes and practice questions, please join our Telegram channel t.me/baayo_official
Your simplicity with words to manipulate makes you out of the box ..Nailed..Bold .. Respect
Simple and classy way of explaining..... really you explained not just protocol but the concept behind it..thanks for the efforts......best wishes for upcoming videos.
Best teacher in the world mam😎😎😎😎
🤗 love love
Tomorrow is my presentation on western blotting technique in college, hope this helps :( I'm memorizing this 🙂
💀 ??
perfectly explained in lesser time, and i have understood all the concept in just 10 min that's amazing
How can you explain such a complex method into such simple language ❤
1. Isolate protine
2 sample preparation
3. Sds page
4. Transfer on blotting pape
Nitrocellulose and PVDF membrane
On of my best channel simply explanation from Pakistan 😊
Incidentally i saw ur video.. and now i m ur fan.. Well explained..u doing such a nyc work for hindi medium students.. Like me..thanks a lot. ☺️🙏🙏
Thank you Akhilesh..
I also
Mam do min to apko dekhne mein hi chale jate hain phir dhyan aata hain padhna bhi hain . Just joke yes i understand your concept easily
Keep uploading ..
Good afternoon ma'am. Welcome again after long time.
Thanks Uday😊
This video is really amazing and helped me to understand the western blot. Thank you very much 😊
THANK U
VEY MUCH
GOOD UNDERSTANDING 🙏👌👌🙂🙂
Thank you for such an easy and accurate explanation.
Nice way of teaching ❤❤ appreciate able ❤
Thank you madam. You told the theory part. I will be very happy if you show the practical also.
I can understand your language very easy.... Thank you so much ma'am 🥰🥰
i love your lecture and the best part is you explain in such a way that everyone can understand
Thanks Saahikanta 😊
MAm m aapse Bhut pehle se padh rha hu.. Aaap Bhut accha Pdhate ho
Thanks 👍
Thank you too
Oooooooo I found, what I was searching, thnx a lot mam, great
Ma'am thank you. App bahut achche se samjhati h aur samjh me bhi asani se aa Jata h. Mai Aapke Sare video dekhta hu. Ma'am Corona virus pr bhi video banaiye.
Thanks mamm you explained it shortly but the way of your explanation is attractive and best......
again thank you so much for all your videos
your teaching style is too good & u explained them all very well👌
Good teacher ❤❤❤lov from Pakistan ❤❤
Thank you mam bottom of my heart
thanks mam for such a nice video.. you explain in very short and sweet way always. please make some videos on antibiotic resistance,and its types of efflux pumps.
thanks again.. keep up..
Noted Geeta..
Thanks for appreciation 🙏🏻😊
You helped me a lot...thank you so much..💗🙂
Too much easy explanation
Wowww maza aa aagaya🤩🤩 bss mam vo Bata dijiye anti rabbit anti body use karenge uska mtb kya h ??
Great Video Ma'am for explaining in the easiest way !
Very good ma'am, thank you so much Ma'am.
Please do make videos on molecular cloning
Thank you very much Ma'am! 😊
Mam thank u very much ur way of explaining awesome
Thank you Mam
You are Gem✨👍
Sorry to saying it im not flirting with you but seriously i cant concentrate on concept while you studying bcoz of ur beauty ....sorry n thank you for giving us valuable information
Thank you
Best of explain
Such a nice and informative video
As always very much helpful mam
Thq mam ✨🎈
Nice video
Thank you Narender 😊
BaaYo Dear Madam I had applied in NIPER for VU-VIS spectroscopy, GC and HPLC chromatography Practical training short courses but there is no replay from NIPER. Can u please help me to recommend other Institutions near Chandigrah who provide me such practical training. Waiting for ur reply
Thanks bhuttt bda thankkuuu.....
I am in class 12 this helps me a lot .....👍👍
Thanks alot✌✌..
Mam i love u .... and your teaching technique ❤❣
Madam,what kinds of protein detection method can be used in western blotting ?
Nice analysis mam
Thank you 😊
Nice ma'am .. plz make a video on cell cycle regulation
Sure..will try to upload before 16th june..
Thank you so much ma'am
Amazing ma'am
Ma'am can you explain on the topic 'schich test'
Nice maam.. Apke vedio se bht help milti h.. App microbiology se h maam ki biochemistry se
You are great
thanks for your lecture
🇳🇵🇳🇵🇳🇵🇳🇵
Amazing mam
Really appreciated 👍👍
Mam app bahut aacha samjhati hai. Mam please bioinformatics ke topic per video banaiye
Please biotechnology ka microscopes. bnaiyega
i have a question. why proteins are transferred from gel to membrane? function of secondary antibody?
Thanks. Please make video on Northern and Southern blotting as well.🙏🙏
Please make the lecture video on food microbiology's topics..
Can u please explain this little slowly? It's too fast. I need to pause everywhere n revise it. But ur contents are good
Why don't you try a feature called *Playback Speed*
Aap speed slow kr k dekhiye....
And here I am watching at 2x 😂😂😂😂
Thank you so much
Thanks for this..❤️💥
Well explained 💜 from 🇵🇰🇵🇰
This video of u is different your voice is more energetic
Than your before videos
Nd looks like u were excited!
So you find it good or not?😊
I dont know but your serious voice i liked that nd ur cool apperance i guess😅in older videos
Very nice ma'am
Ma'am real time pcr and rt PCR na videos hoy to please upload
Mam, I want to know, is it require to again activate the PVDF membrane after the transfer?
Thank you so much mem
Madam you are amazing 👏 ❤ love you 😍
Nice...
Thank u akansha 😊
Mam, please do next video on Mossbauer Spectroscopy.
❤❤ love from Pakistan ❤❤
Love he bhejna... terrorist nhi😂
Vai tum logo ka love bht dangerous hota he,,,,,,,,, tum logo pyr ke sakkar me terrorist vej dete ho 🤣🤣🤣🤣🤣🤣🤣
@@singhania7553 sahi bola aapne 😂
Sare log ek se nhi hote hai
Bhai Pakistan me pharmacy karne ke liye college hai kya ??
Why don't we make primary antibody labelled ?
Thank you ma'am 🙏
Thanks mam...
Thank u Sonali 😊
Informative 😊
Thank you madam 😊
Thnx
Very nice
Thnkew so much 💓
Mam please make a video on eastern blotting
Mam you are the best thank you for your guidence mam I have a question what is the full form of BSA and mam please make a video on CCIEP Please mam
Bovine serum albumin :)
Very very Thanku mam
Mam plz let's know difference between tris base and tris cl.
Too good mam😚😚😚
thank you mam
Anti mouse ya anti Rabbit kya hota hai wese
Mam Gene tagging ka bhi class lejia please
Mam give me a video on ELISA TECHNIQUE
Mam lc-ms pe bhi video bnaye n.
Transfer buffer
Tris base, glycine, methanol,
Hplc k interview questions pe eak complete video banaye pls.
platelet aggregation test maam ,can u plz share
Mam please make on video of michaelis mention equation( most imt part of enzyme).
Mam far western blotting and eastern blotting and zooblotting plz explain
Plz ma'am dna sequences pay aik video bannayee
Sister pharmacuticals company me kis tarah ke instrument use hote he aur company me kis tarah ki jobs aur kis tarah company me groth keya jay us ke mare me vedio kare thanks