Acid-Fast Stain

I also have videos on the Gram Stain, Flagella Stain, Endospore Stain, Capsule Stain and more if you want to check them out! Here is a link to a playlist on all my videos relating to Microbiology Stains:
• How to Pass Your Micro...
(Twitter: @DrWhitneyHolden) The Acid-Fast stain is an important differential stain used to distinguish between Acid-Fast bacteria and Non-Acid-Fast Bacteria, based on the presence or absence of mycolic acid in their cell walls. In this video, Biology Professor will cover the Acid-Fast stain procedure and discuss how it is applied to determine the presence of Mycobacteria, which include the bacteria that cause tuberculosis and leprosy. Great for MCAT Biology Review! For information on the Gram Stain, another important differential stain, see Biology Professor's video: • Gram Stain Procedure

[@taraxxx1993]

Thank you!!!! our Microbiology teacher is really lazy, she teaches us nothing! Its really good that I can watch videos like yours and learn what I need! :)

[@cyannanation5104]

Thank you so much. You’ve helped me for my exam tomorrow. Wish I saw this sooner, but you taught it to me so quickly and I actually understand.

[@cosmic1023]

If you were my professor I'd come to class early and leave late!! Thanks for vid love:)

[@likeaphoenix6]

Why

[@Markzhirkov]

Wow, you are good! I love how easy it is to understand the information. Thank you so much

[@misstalker3979]

came to watch your video to review my Microbio... got an exam tomorrow and I can't just read all about AFS... your's is so helpful... *thumbs up* Thanks! :)

[@SnehaMunni]

M I S S talker czcams.com/video/EsOSAxdGt8M/video.html

[@hiteshsolanki1490]

You are an amazing teacher , watching your videos to pass my Mbbs exams

[@rayna5106]

thank you!! i've been getting really mixed up with this stain, this was nice and simple to learn!

[@BiologyProfessor]

I'm glad to help! I also have videos on the Gram Stain, Flagella Stain, Endospore Stain, Capsule Stain and more if you want to check them out! Here is a link to a playlist on all my videos relating to Microbiology Stains:
czcams.com/play/PLjMo-ys5HQIY2ZL8FDSoabCy4gH9D9rbd.html

[@tgandhib26362]

Thank you so much!! The visual and explanations definitely help!! I will be looking at your other videos as well to be clear on the other subjects in regards to staining. Thank you thank you thank you!!!

[@BiologyProfessor]

That's great to hear that my videos are helping you! :) Check out this playlist for all of my videos on different stains: czcams.com/play/PLjMo-ys5HQIY2ZL8FDSoabCy4gH9D9rbd.html

[@katieyungblut2656]

You explained this so well! Thank you so much for your help!

[@mohsenakhavan3500]

Thank you very much. Deeply Appreciated. I recommend this video who wants to learn about Acidfast

[@76JensStaschel]

You are an amazing teacher, thank you!

[@samarshreshtha1305]

You've explained it so well 👏👏👏👏
Glad I came here

[@krystalhill9463]

Thanks so much I really learned a lot from your videos please continue to make more and keep up the good work.!!!

[@janesebeldina4965]

Your videos are so helpful! Thank you.

[@Devika_p_Jayan]

U makes everything simple and helpful...thankq..

[@shelikestuff]

Amazing , now if my professor could just explain it this way! They make it sooo much more confusing. Thank you!!

[@EDUARDO12348]

you know am very busy learning medical microbiology...but i just had to sign in to like your video. Thank you, you are a great teacher! keep at it

[@SnehaMunni]

EDUARDO12348 czcams.com/video/EsOSAxdGt8M/video.html

[@sunnysun6414]

Thank you very much. This is a great video.

[@bussyjoy]

This is lovely and very helpful...appreciate

[@sandromentetzides2530]

Thats was a great explanation. Thank you. You rock!

[@AbidKhan-pc6wk]

Thanks for share the Z N stain procedure.

[@AhmedElfeky1111]

Amazing video and excellent explanation Thank You
Great job

[@sheikhsadi5184]

Very nicely narrated !! Thanks a lot 😁

[@lamiaazad4654]

Explained so good

[@faithmbewe2952]

You are a life saver

[@sayedalvi9887]

Amazing explanation, bravo!!!

[@apearson79]

This is exactly what I needed!

[@BiologyProfessor]

Great! :)

[@akbarhamdard5877]

please make more videos, you did a great job!

[@SnehaMunni]

Akbar Hamdard czcams.com/video/EsOSAxdGt8M/video.html

[@Majikabz1800]

Great video!

[@asyouwant3643]

Awesome teaching.....

[@dr.nageshwarhulgunde3378]

THANK-YOU. YOU MADE IT SO EASY.

[@annanicolesolitaire7804]

Thank you!!!! This helped out a lot!!!

[@BiologyProfessor]

I’m glad 😀

[@andyalbertochavez5135]

This is awesome, thank you!

[@BiologyProfessor]

Glad you like it!

[@RodesLaw]

Can you do a video on Counting CFUs? Determining Bacterial numbers.
Thanks for these informative and well though-out videos.

[@pkthe2ndhokage188]

Thank you!

[@sallyrahma608]

Thank you

[@tessafaithlong2394]

Super helpful, thank you!

[@zakirkhankausar]

Amazingly explained 💯👏😘.

[@BiologyProfessor]

Thanks a lot 😊 - please subscribe and tell your classmates about this channel for studying. :)

[@cabdulaahidiireye5197]

thanks alot, idid not seen like you.

[@teamrpzerrorgamer6460]

thank u this helped me a lot!

[@sayedbasuny140]

great explanation 👏

[@sanjeev4061]

Excellent video ❤

[@clairemonaghan9684]

Fantastic. Thank you!

[@sci8469]

Thanks

[@brittanyzwerk3234]

Super helpful thank you

[@donroy6496]

Thank you.... So much

[@rajasekar6068]

Thank you sooo much

[@kylayu1065]

2022! Still a very great video for study this

[@BiologyProfessor]

Yes, it has aged well. ;)

[@sal4439]

thanks for this, rlly helped

[@mslovelee86]

Thanks!

[@madra7713]

huge thank you 🙏🏼🙏🏼🙏🏼🙏🏼🙏🏼💙💙💙

[@SaiwanMaerufSaiwanbio]

thanks for your video , but i think you missed mention about heating when you add strong carbolfuchsin , it is crucial step in AFB stain

[@Youngflynsaved7]

Wow thank you

[@SherineYousery]

Thank you so much. It's important to emphasize that the Acid-fast stain is Ziehl-Nielsen's stain so that beginners in Microbiology don't get confused.

[@231mac]

Yeah... that was a kinda dumb request, lol.

[@younnaa4702]

Thank u so much

[@amirdm4585]

thnx for sharing this. thumbs up

[@mohammedabdulwahidhassan485]

Thanks finally i understand it

[@grishaasher7653]

SHE IS SO FREAAKING GOOD.LOTS OF LOVE
I WISH YOU WERE MY BIOLOGY PROFESSOR

[@barbaralarissa]

THANK U!

[@medic8038]

Good job professor 👏🏼👏🏼👏🏼👏🏼💜

[@BiologyProfessor]

Thanks :)

[@drmichaelopare8245]

thank you very much

[@ghadahalyami1852]

So much helpful!

[@BiologyProfessor]

I'm glad! Thanks for watching! If it would be helpful, check out my playlist of videos on different kinds of stains, like the Gram Stain, Endospore Stain, and Flagella Stain: czcams.com/video/9mpivFwDfC8/video.html

[@baselr4021]

Thank you
it is helpfull for me

[@SnehaMunni]

B R czcams.com/video/EsOSAxdGt8M/video.html

[@fountainvalley6763]

It's awesome. tks

[@achibida9591]

thanks

[@sindhusabu6760]

Thank u madam... Usefull video😁

[@bashirah4454]

Thnks

[@peterbadalamenti8240]

god bless you!

[@laithkz4403]

Nice thanks

[@syntacticcave9514]

Great

[@anabellabella2266]

THANKS!!!!!

[@jorgemejias5960]

Awesome thanks

[@Malikaafghan]

Also, could you please make a video on CFU?
Thanks again :)

[@BiologyProfessor]

I'll add it to my list, but it might be a few weeks. Thanks for the suggestion!

[@iDomoPolyForums]

I would recommend that you make another channel called Microbiology Professor, or just include that word in the title.

[@latifaabdulatif1426]

perfect!!!!!

[@SMass-ey8er]

thank you :)

[@BiologyProfessor]

You're welcome!

[@Malikaafghan]

Wonderful explanation! Thank you so much :)

[@Infidel_Jay]

so during an acid fast stain what color would the gram negative stain if a non acid alcohol were to be used as a decolorizer? clear?

[@BiologyProfessor]

probably red - without acid in the decolorizer, the carbol fuchsin would not leave the cells.

[@njoodsaleh1365]

Thanks 🇸🇦♥️♥️

[@adilfarooq5582]

I like your vidoes

[@user-uw7li3ht4i]

Love it thank you could you please do like this video with all stains🥺❤️

[@BiologyProfessor]

What stains do you need?
Here’s my playlist to all my stain videos: czcams.com/play/PLjMo-ys5HQIaQWhxrInBxjMjuJOsNhqSz.html
I’ve got:
Gram Stain: czcams.com/video/1SITCrgF3yk/video.html
Acid Fast Stain: czcams.com/video/TCFpU8p3By4/video.html
Endospore Stain: czcams.com/video/9mpivFwDfC8/video.html
Flagella Stain: czcams.com/video/tjaWQELBu1M/video.html
Capsule Stain: czcams.com/video/_dVc65fwoSs/video.html
Acidic and Basic Stains: czcams.com/video/pGDBAO6oINY/video.html

[@sajjanraja3070]

perfect

[@BiologyProfessor]

thanks :)

[@MdJamal-sc9iu]

Micro blb bg zn afb stainings not ditctiva ka Matlab Kya hei

[@584emad]

leprae is a gram positive so how can you use acid-fast stain to detect it?

[@BiologyProfessor]

Based on its cell wall structure, Mycobacterium leprae is a gram-positive, rod-shaped bacteria with a thick waxy coating. Due to this coating with mycolic acid, it cannot be stained very well with the regular Gram stain procedure. That is why we use the acid fast stain on it instead.

[@saskiakristofedes2482]

hi . i have question. why do some lab use malachite green as a secondary stain ???

[@BiologyProfessor]

Personal preference, I guess.

[@231mac]

Our lab did whenever we ran out of Methylene blue, lol. I think under certain microscopes Malachite green showed up better.

[@mikeo.4203]

I think you should go back and redo some of these videos as I feel it's important to mention what the mordant is for example.

[@narendrashelke7012]

hello prof. if the bacteria is non acid fast then it is gram negative?

[@BiologyProfessor]

Not necessarily. You would need to actually do a Gram stain. Non acid fast bacteria can be Gram positive or Gram negative. The acid fast stain just shows that non acid fast bacteria don't have mycolic acids, but it cannot assign their Gram type.

[@virtuality400]

Teacher you didn’t mention ,about the heat part which acts as a mordant 😢😢

[@berniedmjm4108]

What outcome (color) is expected for a Gram positive, non-acid fast bacterium like Staphylococcus aureus if alcohol is mistakenly used instead of acid alcohol?

[@BiologyProfessor]

Alcohol is not good enough to remove the carbolfuchsin dye from non-acid fast bacteria because carbolfuchsin isn't very soluble in it. So using alcohol instead of acid alcohol will not decolorize properly and more non-acid fast bacteria will appear red. Hope this helps!

[@berniedmjm4108]

Awesome!!! Thanks!!!

[@mawimawiizathang9589]

Why is ZN stain used instead of Gram stain in spite of the organism being a bacteria?

[@BiologyProfessor]

Acid-fast bacteria don’t stain well with Gram stain because of the waxy mycolic acids in their cell wall. So another stain is necessary.

[@aprilespinoza88]

You are awesome. :)

[@vishnupriya9229]

Why used acid fast staining for actinomycetes

[@BiologyProfessor]

To distinguish it from Nocardia. Actinomyces and Nocardia can be difficult to distinguish without staining. Acid-fast Nocardia can be distinguished from from non-acid-fast Actinomyces with an acid fast stain.

[@epheriaplace3002]

Why is acid alcohol used as a decolorizer in an acid fast stain, as opposed to a neutral alcohol solution?

[@BiologyProfessor]

because carbolfuchsin is a basic dye that is soluble in acidic solutions (like acid alcohol or the mycolic acids in the acid-fast cell walls)

[@epheriaplace3002]

thanks for reply Prof. So whats advantage of alcohol? pls

[@romikarki429]

what are the reasons for false positive result?

[@BiologyProfessor]

Several things can result in a false-positive, including cross-contamination between samples in the lab, contamination of diagnostic equipment, and improper use of the staining protocol, like not treating the smear with decolorizer like enough.

[@saikumar802]

SPECIAL STAINS* -
Lipid accumulations*- Oil red O stain
Sudan black B or IV
Osmium tetroxide
Glycogen* -Best carmine
PAS
Melanin* -Fontana masson staining
Hemosiderin* -Perl's prussian blue stain
Calcium* -Von kossa*
Alizarin red
Calcein stain
Tetracycline labelling index*

[@shreeshyampareek5154]

Why we use 20% H2SO4 for M. tuberculosis when 5% can wash the background as in case of laprae.?

[@BiologyProfessor]

I don’t know. I’ve never heard of this, I’m sorry.

[@shreeshyampareek5154]

@@BiologyProfessor ok🙂

[@abdullahbutt2863]

Loved ❤️ from Islamic Republic of Pakistan 🇵🇰. May Almighty Allah bless you sister

[@BiologyProfessor]

Thank you.

[@IK-fg4gw]

👍

[@allanochoa4826]

question:
i was working on an unknown bacteria and i was doing this procedure to identify my organism. I had a positive control on one end of the slide and my unknown on the other end; when i performed this procedure my control positive also came out blue! why!? my unknown bacteria also came out blue; so the control was compromised and i had to start over.

[@BiologyProfessor]

Maybe you used too much decolorizer or left it on for too long? Here is a link to some other troubleshooting tips: faculty.lacitycollege.edu/hicksdr/staining.htm

[@savitakadian6318]

Allan Ochoa

[@vigneshsfc9404]

Super mam useful video

[@BiologyProfessor]

Thanks! Tell your classmates 😉

[@vigneshsfc9404]

@@BiologyProfessor yeah sure