How to draw (GO) and KEGG pathway

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  • čas přidán 25. 08. 2023
  • #howtodraw #geneontology #kegg
    In this video, I have explained how we can draw GO and KEGG terms using , using SR plot website.
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Komentáře • 25

  • @user-wc6hj8yr2l
    @user-wc6hj8yr2l Před 11 měsíci +1

    Great job 👏

  • @ottonecarmeloviscardo3674
    @ottonecarmeloviscardo3674 Před 10 měsíci +1

    Thanks so much for the video. If in the same pathway I have both up and down genes, how could I do?

    • @asifmolbio
      @asifmolbio  Před 10 měsíci

      You can make a heat map to show up and down in different treatments

  • @kashifhameed2650
    @kashifhameed2650 Před 10 měsíci +1

    Thank u Dr Asif for video. Plz elaborate data preparation, we could not understand, where from and how did u selected KEGG terms, KEGG categories, p values, count and regulation. Sir plz explain how can we obtain these informations from the data we received from BGI company. Also plz tell us how we can find out statistically significant DEGs. Thanks alot.

    • @asifmolbio
      @asifmolbio  Před 10 měsíci

      If you are new to field this video will be helpful

    • @asifmolbio
      @asifmolbio  Před 10 měsíci

      Interpretation of RNA seq data | DEGs | GO | KEGG
      czcams.com/video/xc2zEI_5pmw/video.html

  • @belief6695
    @belief6695 Před 6 měsíci +1

    Sir.. after submission.. we are not getting result.. its showing error

    • @asifmolbio
      @asifmolbio  Před 6 měsíci

      You prepared the same file as i prepared in the example?

  • @muhammadrizwan7989
    @muhammadrizwan7989 Před 9 měsíci +1

    i am trying on this , but it show file was not found

    • @asifmolbio
      @asifmolbio  Před 9 měsíci

      There would be problem in the formatting of your file and data format

  • @asmaaammar1781
    @asmaaammar1781 Před 8 měsíci +1

    The colors not changed all pathways down or up the same colour and i can not solve the problem

    • @asifmolbio
      @asifmolbio  Před 8 měsíci +1

      You can make separate graphs for up and down. This would be the best way to

    • @asmaaammar1781
      @asmaaammar1781 Před 8 měsíci +1

      with the same colors all colours are gray and I can not change it how can I share my photo to you@@asifmolbio

    • @asifmolbio
      @asifmolbio  Před 8 měsíci

      asifalikalas@foxmail.com

  • @nedafiroz514
    @nedafiroz514 Před 10 měsíci +1

    Thank you for this video, How to interpret which gene is responsible for stress after gene ontology because I received 165 gene set and I can't find the proper instructions to interpret how to find specific gene? Can you please help through a video

    • @asifmolbio
      @asifmolbio  Před 10 měsíci +1

      This you should study literature first which genes are involved in stress. Like WRKY transcription factors are involved in stress. You can try gene ontology enrichment analysis by GO shiny tool and make pathways.

    • @nedafiroz514
      @nedafiroz514 Před 10 měsíci +1

      @@asifmolbio thanks so much sir for the reply. If I have further questions, how can I contact you again

    • @asifmolbio
      @asifmolbio  Před 10 měsíci +1

      asifalikalas@foxmail.com

  • @user-vy6fg3gx8w
    @user-vy6fg3gx8w Před 10 měsíci +1

    Sir how can we calculate the p value of a protein molecule easily

    • @asifmolbio
      @asifmolbio  Před 10 měsíci

      How to calculate FC, log2FC, Pvalue, Padj, Up/down genes in RNA seq data using Excel
      czcams.com/video/HH3Mll4W5WE/video.html

  • @rimshaqazi2886
    @rimshaqazi2886 Před 7 měsíci

    Sir is m result jaldi nhi ati ....registration b k hn ....

    • @asifmolbio
      @asifmolbio  Před 7 měsíci

      Which analysis you are trying to

  • @rimshaqazi2886
    @rimshaqazi2886 Před 7 měsíci

    Abhi tk hmi koi b result mili nhi hn

    • @asifmolbio
      @asifmolbio  Před 7 měsíci

      You mean website is not working?