Bacterial Identification Tests: Motility Test (Hanging drop method)

Motility is the ability of an organism to move by itself by means of propeller-like flagella unique to bacteria.
If fresh culture of bacteria is available, microscopy is the most accurate way to determine bacterial motility and ‘Hanging drop method’ is a commonly used microscopic technique.
Hanging drop preparation is a special type of wet mount (in which a drop of medium containing the organisms is placed on a microscope slide) to observe the motility of bacteria.
This Video demonstrate Procedure, Observation and Use of Hanging drop method for bacterial Motility test.

[@renish1531]

This video is very helpful i will recommend it to my friends . The motility test was my weakness but not anymore.

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@SatyamKumar-tn4nt]

Excellent explanation
Now my concept is clear and I can give viva without any fear
Thank you sir 😊

[@the_bioway]

I'm glad that this was helpful for you🙏🏼
Best of luck for your Viva👍🏼

[@ghaidaghaida3742]

Very helpful. In college we use the play dough instead of cavity slide. I did this experiment 4 times and I couldn’t observe any motility. So my teacher couldn’t give me the mark. I think that’s because we fix the lens on 100x power , I will try again with 40x power. Thanx

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@rithikmodi4468]

Hi direct second year students

[@roshangaikwad7962]

“I like your video!”

[@the_bioway]

Thanks

[@pavadaiarumugam9723]

Very clear explanation sir. Thank you so much

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@BalogunRoseline-oj3zn]

Thanks for this wonderful teaching

[@the_bioway]

Glad it was helpful!

[@subhdrasahu3273]

Wow ❤ Sir this was so interesting and helpful🎉thank you so much

[@the_bioway]

Glad you liked it!

[@santhosh30899]

Thank u so much sir the video was really helpful❤

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@sumaiyyahashmi6623]

But we are not dropping any thing than why its called as hanging drop method

[@the_bioway]

Because bacterial sample we have taken on coverslip is now in inverted position against the cavity side.... when we we observe it under microscope ... Which results into drop hanging from coverslip

[@Josephhell43]

Thanks

[@Mr_humerus462]

Excellent.. Thank you🙏💕

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@priyakotian1264]

Sir.. You have to off the bunsen burner flame

[@usmanrafilu7386]

But sir why the video procedure are not displayed?

[@ammarsayyed1635]

Awesome video sir

[@abhitoons5213]

Ham to fan ho gaye apke 🎉

[@the_bioway]

I'm glad that this was helpful for you.🙏

[@welearner4402]

nice video sir, its really helpful

[@the_bioway]

I’m glad that this was helpful for you 🙏🏼

[@riyapopat9934]

Thank you so much

[@the_bioway]

I’m glad that this was helpful for you🙏🏼

[@christinahinton4538]

excellent. thank you Sir.

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@kalanidhih749]

Sir , why we focuse the edge of the drop in this method ? 🤔🤔

[@the_bioway]

There is higher probability of finding motile bacteria due to oxygen concentration at edge

[@kalanidhih749]

@@the_bioway Thanks a lot sir 😊

[@buddy7270]

Thank you so much sir ❤️

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@sheraziz147]

Very informative video

[@the_bioway]

I’m glad that this was helpful for you 🙏🏼

[@sanarahim782]

Wgat if there isnt any cavity , will we be able to observe bacteria mobility.

[@ritwikghosh7743]

Thank u Sir. Great explanation. One question, can plasticine be used in place of petroleum jelly?

[@the_bioway]

I'm glad that this was helpful for you🙏🏼
Coming to your question... Yeah you can use Plasticine provided it should hold cavity slide and cover slip properly.

[@ritwikghosh7743]

@@the_bioway Alright Sir, thanks😄

[@rithikmodi4468]

Hi malhari how are u

[@rithikmodi4468]

I love microbiology

[@user-hf1sn1pw1m]

God bless you💙

[@rekhashinde7015]

Sir you took another cover slip but didn't applied on slide na 😕

[@zahramohammedhamedalmahroo1548]

Incredible

[@SandeepSir1310]

Nice explanation...

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@akankshamadavi8919]

Plz tell the principle of hanging drop method.

[@ritashreebasu9806]

If you open the cover slip with sample of bacteria in air for a long time that u do now then bacteria could be change their character and started to reaction with environmental organisms...how can do u this process... sir??

[@Munde0608]

Nice Sir

[@kiranbhagat789]

Nice video

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@eleven9701]

Sir Do we need to use the fresh broth for viewing motility assay ...or can we use the refrigerated broth also

[@the_bioway]

Freshly prepared one.

[@babluyadavbabluyadav8536]

Very nice 👌👌

[@the_bioway]

I’m glad that this was helpful for you🙏🏼

[@pharmed6914]

Hlo is it possible to view the border in 40x too or in just 10x.. My teacher keeps asking for that in observation.. I'm not really able to get it.

[@the_bioway]

At 40x or 10x you can see only the border but to see bacteria you need to go for higher magnification power

[@aliyusaniado8807]

Its helpful

[@the_bioway]

I’m glad that this was helpful for you 🙏🏼

[@ab21234]

Sir we can see any type of bacterial movement through this procedure?

[@the_bioway]

Yes

[@anushreekor1648]

Why did u use petroleum jelly only to coverslip?

[@the_bioway]

To make sure only corners of coverslip getting attached

[@restlessmedicos785]

I am worry for the subject from whom this sample is taken😩😩😩😭

[@CC2k12]

does this method need heat fixation?

[@the_bioway]

No

[@fathielzahra]

How to do this experiment with solid media

[@the_bioway]

You can refer following article for Motility Test using Semi-solid Media
microbeonline.com/tests-bacterial-motility-procedure-results/

[@fathielzahra]

@@the_bioway Tomorrow is our microbiology lab exam. But I'm still confused about making hanging drop from a solid media.

[@the_bioway]

Hanging drop method doesn't use Solid Media.

[@arivusaravanan7327]

I have a question
Why do bacteria move in edged

[@the_bioway]

Bacteria move towards edge due to high oxygen concentration at edge

[@arivusaravanan7327]

Tq so much for your certain help.

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@zahraa6475]

Sir please can Answer me the heating of loop donot kill bacteria ?

[@the_bioway]

Heating the loop kills unwanted bacteria... So it becomes sterile (free from bacteria) and after cooling it...then it can be used to transfer bacteria of our choice....

[@zahraa6475]

Thank you sir god bless you

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@priyakotian1264]

No.. We have ro sterilize the loop.. We are doing many cultures and and we have to transfer many of the culture to cover slip.. So we have to sterilize it

[@msdohni9369]

Heating of loop kya huta hai mam

[@rithikmodi4468]

Hiii

[@rithikmodi4468]

Malhari how are u

[@ayrabatool]

❤

[@zarin1897]

💕💕💕

[@PiyushKumar04141]

How to report?

[@the_bioway]

Reporting is done as Motile, non-Motile and sluggishly Motile organisms

[@komaltiwari1407]

👍👍👍

[@the_bioway]

Thanks

[@brookarsenault1259]

can I hear a cat in the background. I hope not.

[@the_bioway]

Not at all... 😅

[@dannyguzman5142]

cats are great

[@i96i9]

ايزي😂😂😂😂😂😂😂😂😂😂😂😂😂😂😂😂

[@babluyadavbabluyadav8536]

Thanks

[@lalitpandey240]

Nice video

[@the_bioway]

Thanks

[@drx_prem_nayak143]

Nice sir

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@medicos5649]

❤️