Sub-culturing cells || Cell culture basics
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- čas přidán 14. 04. 2021
- Sub culturing (aka passaging), is the removal of the medium and transfer
of cells from a previous culture into fresh growth medium, a procedure that enables the further propagation of the cell line or cell strain.
The growth of cells in culture proceeds from the lag phase following seeding to the log phase, where the cells proliferate exponentially. When the cells in adherent cultures occupy all the available substrate and have no room left for expansion, or when the cells in suspension cultures exceed the capacity of the medium to support further growth, cell proliferation is greatly reduced or ceases entirely . To keep them at an optimal density for continued growth and to stimulate further proliferation, the culture has to divided and fresh medium supplied.
thank you for taking your time for making this video!!!!!!!!!!!! I am doing a internship in a lab where I am learning how to do cell culture but it is very intimidating at first,,, but this explained SO MANY CONFUSION.. THANK YOU FOR BEING YOU!!! wow
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So much helpful and excellent presentation
Really good video! I have watched some before but this one provides the most clear information. Thank you!
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well explained!! a surplus knowledge need to know.. thanks a lot.......😍
Well-explained, thanks 😊
Thanks alot for this! You are a blessing for biotechnology and microbiology students!
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@@animatedbiologywitharpan yes I will always ✨
well explained, thank you very much.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thankyou for such informative video!
Please share my channel link with your friends and help me to reach big audiance
Thanks for these topics, as ur video's helps in clear understanding off topics❤️❤️
More videos would be uploaded soon.....stay tuned
Waiting for u on unacademy
@@cartikey4687 due to covid lock down in Mumbai I might relocate to my home town and then be active again
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how to know the split ratio that I need to use to seed the cells?
Thank uhh fr such a great explanation
Please share with your friends
Hello,thanks for your amazing video.It's really useful in my experiment.But can you teach us about MTT assay this experiment?
Thanks for your suggestion....I will upload a video on MTT assay soon.
Nice presentation thank u , so helpful ..upload more videos ..pls
Check out the cell and tissue culture playlist
czcams.com/play/PLKtiwIJ8Q7rpNrZRxzBgoH2Q6oz4bJwg2.html
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Thanks!
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Loved it.. good understanding
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You are awesome !
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Do you use BioRender? Nice video man best of luck.
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thankyou sir
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Nice vdo. 👌👌
Are you on unacademy??
Yes I am there
at what rpm do we centrifuge
200-300 rpm is good to precipitate cells
Sir primary secondary sub culture ki video khaaan hain ? Ye tu btain ..bht aala parha rai ap ...
Cell culture playlist czcams.com/play/PLKtiwIJ8Q7rpNrZRxzBgoH2Q6oz4bJwg2.html
Aala parha matlab ? Mai samjha nahi
Sir aala ka matlab hai bht acha...concepts clear ho gai .if i started listening u one day i will become microbiology expert 🥰🥰🥰🥰
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Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
god bless indians
Please share my channel link with your friends and help me to reach big audiance
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