Lab Skills: A Common Micropipetting Mistake

A micropipettor is only as accurate as the person using it. Poor technique leads to poor results.

[@geos7432]

Hello!!! Thanks for the tips. Could you make a video on filter sterilization (of gibberellic acid-3 for example)? Regards!!

[@ItsLearnable]

I won't be doing any filter sterilization this semester, so I won't get a chance to record anything like that for a while. You might find this video helpful: czcams.com/video/M__bFPnmVcE/video.html

[@mariaph9700]

is the first and second stop applicable for air displacement pipette only? We used positive displacement today and it appears that it doesn't have first and second stop.

[@ItsLearnable]

Yes. In positive displacement pipettes, the piston makes direct contact with the liquid being measured, so there is no "extra air" to blow out.

[@mariaph9700]

@@ItsLearnable ah it makes sense now, thank you so much!