Deflasking the "Rarest" Aroid from Tissue Culture | Philodendron Spiritus Sancti
Vložit
- čas přidán 4. 04. 2023
- G'day, guys! In today's video, I deflask some Philodendron erubescens 'Red Anderson', El Guapo, and the "rarest" aroid indoor plant out there, the Philodendron Spiritus Sancti. Connect with me on Instagram - / petevsplant
Check out my eBay store - www.ebay.com.au/usr/petevsplants
Deflasking tissue culture plants may seem complicated and daunting, but I assure you, it's not that hard at all. Today, I show you how to put together the pots, the substrate (coco coir and perlite), and the process of sterilising the tissue culture plantlets before planting them up. This is how to deflask the 'rarest' indoor aroid plant Philodendron Spiritus Sancti.
As always, if you have any advice, leave it in the comments below!
Wow,how exciting the Spiritus.I wish you luck to have many healthy babies.I love this,the tissue culture that is.And how it brings us poor people beautiful plants.Thru SCIENCE!!!!!!!!!
Hehe thanks Alice :D
I miss the videos Pete. I keep rewatching them because they're that good. 😊
My TC plant babies are some of my most treasured plants ! I love being able to grow a tiny plant into a massive plant, it's really validating as a plant owner
Definitely :D
Well done Pete! Looking forward to further updates along your journey with these TC babies. Exciting times!
Cheers, Marcia! I'll try and have some updates in a month or two :D
This is so cool- thank you for sharing! I love seeing science make previously inaccessible plants become available for a wider audience! exciting to see the rest of your journey!
I've never even heard of tissue culture's before. I always learn something from your videos !!❤
:D
Roots rotting off makes sense: agar is constantly moist of sorts. They need a different type of roots when transitioning to soil or water.
Yep
Aloha from Maui Pete, Well Done!!!!! **note: on prop boxes, or “Hotel de la Totes”, I heat a metal poker or extra large nail, hold with pliers & heat tip with gas torch or gas flame, & poke 3 or 4 holes per each side of tote. ***drilling seems to catch & crack plastic tote*** Humidity will stay extremely high, but not excessive, & less chance of plant leaf rot….. Aloha
Interesting. Might have to try it :D
I recently ordered a spiritus sancti in TC from Thailand! Mine should be arriving tomorrow :) I may or may not have a very similar video coming out soon lol
Hehe awesome. Hope it goes well!
Oh btw, I forgot to mention I love your channel and have been subbed for a while now :) If you have any feedback on what I did here (i.e. anything very stupid) please feel free to let me know haha :D
How exciting Pete! You're quite the adventurer😆Well done and I look forward to updates 👏🏼👏🏼
Hehe gotta have fun with it :D
Finally some exciting plants ❤❤ keep us updated
Lol finally? hahaha
Great video Pete! What a nice haul you got can’t wait to see how they grow 😊😊😊
Thanks mate!
Well done Pete, so informative and transparent. All the best 😀
Cheers, mate!
Oh wow......i have so much to learn!!
Hehe just begin :D It's good fun!
It’s so interesting to see what you’re doing
Always something no one else seems to be attempting, good luck, hope it doesn’t take too long for them to grow into plants
Again thank you for letting us into your plant journey
Thanks mate! They're still alive and thriving as of a week later, though the El Guapos are looking a little worse for wear.
You are amazing thanks for your videos and information I appreciate everything you do and helps us to be a better plants parents keep up the good work from Pennsylvania
You're too kind, Victor! Are you planning on doing any TC stuff in the future?
Nice work cheers for sharing. On the plastic clear cup thing, its rather ridiculous right especially when the biggest polluter is the likes of the plastic coke bottles and packaging etc in the super markets. Crazy world we are living in
Wow can't wait to see updates!
Will do in a few weeks/maybe a month :D
Love El Guapos...they are gorgeous and they are crawlers too...with each leaf they get bigger and bigger ❤
Yeah, I'm dying to get a large one at some point :D
@Pete vs Plants wish could send you a pic of mine...5 leaves and booming
Hehe send me it on Insta :D
Looks good to me, but I know nothing about that process! Can’t wait to see updates. Thanks for sharing Pete!
Cheers, Carol! Hopefully I'll have some good updates in a couple of months
Bruv I myself would love to get into TC plants, it does seem like a little work but well worth it in the end. I almost bought a bunch of TC Joepii plants to grow out but I decided I didn't have the gear to work with them but then I met a fellow in Washington and he did it in his room and sells some amazing little plants.
very interesting video. Enjoyed it. Thank you.💛
Cheers, mate!
If you’re looking for disposable plastics, try the $2 dollar stores in your area. They usually hold onto those sorts of things. There’s also been floating around in Daiso (japanese $2 store, not sure where in Geelong but def in the CBD and some bigger shopping centres) these plastic pudding cups that make a perfect humidity dome and base! Hopefully that helps.
I’ve been recycling Starbucks cups for my propagations, they’re a perfect wide mouth with a lid, plus recycling. Plastic is definitely disappearing (thankfully!)
Daaaaad! Nnnnnno!
The Happy 21st stories begin.
Hehehe
Poopers,I'm sooooo excited- I can't wait to watch them grow.
:D
Can't wait for the Red Anderson.
I know! :D I just want a big one already haha
🐸🧦
I'm excited for you, and your future endeavors. I hope you make a pretty penny. 😊😊
Haha thanks mate
Thanks for the awesome videos Pete! I'm working on some of my first tissue cultures and your videos have helped a lot for them and also my many other aroids and such. I started acclimating my first batch a few weeks ago and now have 3 more different kinds working on acclimating out of the agar now. So far so good.
Thanks again for all the great content I look forward to seeing updates on these!
My pleasure, mate! What are you taking out of TC? Hope it goes well!
@@petevsplants7516 I have some White Wizards, White Knights, Peraiso Verde, Billietiae going so far. Nothing to fancy to get started. If all goes well I will try some fancier kinds.
Very cool! I've done the WW and WK out of TC and they've done well :D
@@petevsplants7516 that's great to hear! My WW look to be getting ready to start growing. A couple have some beautiful variegation. The others are fresh out of the agar one week ago. So they will probably take a little while yet before I notice anything exciting. The Peraiso Verde also have awesome colors. I'm definitely glad I ventured into the TC's
Yeah it's a lot of fun. Love seeing things grow from such tiny beginnings :D
Spiritus grow like weeds. I have one in a 4in/10cm pot with roughly 7in/18cm leaves & it gives me a new leaf every month. even the aerial roots grow quickly so I’m not surprised that your plantlets are already growing aerials
Wow! Good to hear :D
cool!!! yes I'm definitely terrified of TC 😂😂
Hehe you don't have to be. Definitely recommend giving it a whirl.
I'm very keen for the spiritus to become available. May have to purchase one off you once they're established. Big wishlist plant!
:D definitely!
Really looking forward to updates on this fascinating video.
Just curious if you could have tried a couple of the tc’s in your aeroponic system?
I think it could work, but they'd have to be big enough to fit in those rubber inserts properly so the roots could get access to the water/air below
Lmao 🤣 floor space looking like the corner of my living room 🤣 I'm not alone 😁
Lol trying to keep it real
Tissue culture is so fun! I used water to wash, peroxide an Physan 20/20. Worked great!! Good luck with your new babies💚💚💚
Thanks Sherry! What were the tc plants you were doing?
@@petevsplants7516 PPP Marble 💚
@@sherryporsch9349 hehe very nice!
Cool vid Pete. Kinda surprised you didn't throw a few of these into your "turbo" propagator system. Seem like you'll get the most amount of roots quickly that way. Also would using rooting gel help or is there enough hormone from agar cocktail?
That was fortunate enough to get my spiritus from the seedling from a grower here in the US for about 500 usd thankfully, and another, but TC over Black Friday 2022 for 250 lol
Wow, crazy. They're still ridiculously expensive here.
Best of Luck on your PSS Babies & others
Thanks mate!
Defs recommend inoculating with mycorrhizae, something broad spectrum after deflasking. At least from my experience with orchids.
Interesting. I guess you have to do it in the soil and not include a fungicide after you've soaked the plants or it may defeat the purpose.
Exciting times ahead for a few lucky Australian plant parents. Well done Pete!
Was wondering, you kept everything clean for the plantlets, would it be necessary to sterilise the growing substrate? 🌱💚
Yeah, ideally you'd probably do that too by baking it. Though, I've never had a massive problem (in my limited experience) from the substrate assuming it's not soil. If it were soil, then you'd probably want to definitely sterilise it.
@@petevsplants7516 it was something that came to mind as I was watching. I did get a few tc a while back and that was the only thing I didn’t do either, in fact didn’t even occur to me till was watching how carefully you were treating the plantlets. Keep up the good work mate. Looking forward to watching them grow with you 👍🌱💚
Cheers, Kathy
Hi Pete, I'm a big fan of your videos and watch them whenever you release them. I have a question what's the point of using hydrogen peroxide immediately after removing from the sterile flask? Isn't it an extra step? Don't you think that might negatively affect the new roots? When deflasking orchids we just wash it in water and fungicide could be sprayed later on or same way you're doing it.😊
Hey Mary, cheers for the question. I'm honestly just following a 'recipe' I was given, but you make a good point. It seems to have worked okay so far, so I guess at worst, it's not having much of a negative impact.
Honestly Pete, I’m more worried about you handling the copper fungicide like that than what it might do to the plants, cute as they are! Didn’t the el guapo used to be sp columbia ? That one is a beauty
Hey mate, I'll read up more on it though I was careful to repeatedly wash my hands and clean down surfaces and utensils afterwards. Yeah I think the El Guapo was the sp columbia.
33:00 Peaches don’t give a rat’s ass 😹
🍑🍑🍑
lol clearly haha
Where do you get the shredded coco coir? Love your videos and I am totally going to try my hand at TC plant babes!
Hey mate it's at Bunnings Warehouse.
What happened to your living wall? You did such a great job a while back. :D Says the lady who reorganizes her plant room on a monthly basis cause I can't keep it organized. LOL.
The plants from a flask are already sterile when you take them out, so you don't need to sterilize them again. Just wash off the gel and slowly acclimatize the plantlets to your growing conditions.
Then again, the fungicide could help the plants if there are gel residues on the roots.
How have they been handling the pot up ? I’m thinking about getting some from greenboog but I’m not sure I’m ready. For that yet lol
Heya Pete, tissue culture plants are sterile, so I’m curious about why you’re hitting them with peroxide. The copper makes sense as a prophylactic if you don’t rinse it off.
What’s your opinion on acclimating TCs in stratum as a substrate? I hear it’s been really successful for those who have done it.
I honestly haven't tried it yet haha I bought the stuff but just haven't had time. Recently, I found coco coir can be a mixed bag, because it lacks nutes if your plants are hungry and growing fast, you have to fertlise or move to soil quickly which can be a pain.
Very cool! Where would you recommend starting to look for tissue cultured plants?
Yeah definitely. Look for something in your price range for playing with that you wouldn't be too upset with completely losing the first time you try :D
@@petevsplants7516 good advice - haha! I actually meant, ‘where’, as in websites, etc. I’m in Korea and have a similar idea to what you’re doing - introducing new varieties to the local market. It’s been a hobby for 10yrs, now exploring it a bit more seriously.
Thanks for the content - informative AND entertaining!!
@@nathanmillard Ah sorry, my bad. My business partner does all this in China and Thailand. So, I imagine just starting to Google TC labs and then making contact with them and asking for price lists, etc. should set you up. There're loads in Thailand.
Is there an update on these babes?
No personal experience, but I see a lot of people rooting pups and qorms in fluval stratum very succesfully. No root battles like with sphagnum and both are pretty sterile. I worry about emerging roots staying moist enough in your soil mix.
Vermiculite is known for developping roots and seedlings. Another option?
Yeah I've seen that too, though, no experience with it.
Fluval stratum is my go to now for props. I’ve not tried TC yet though. It’s also great mixed equal parts with perlite and moss in prop boxes - the moss doesn’t bond so tightly with the roots. MST comes off with a gentle shake but the stratum and perlite chunks make it super airy and loose. Worth mentioning though that the stratum /perlite/moss mix looks a sight due to the blackness being absorbed by the moss. Corns root and sprout leaves in 2-3 weeks. My various wet sticks activate far faster than with pure moss prop boxes. My prop boxes just get window sill light not grow lights.
Where do you get tissue cultures?
Thailand :)
I always get so jealous of the plants you get haha, i buy some new ones every few months so i don't give my husband a great attack 😂😂😅. I wait until they drop a little in price before getting em. I love the spiritus sancti, but its to much for me right now, same with the el guapo, so beautiful. But maybe next year im thinking. I yried to buy tissue culture white wizards, and they all died, i wasnt to upset, i was out like 60 bucks. I just went ahead and bought a acclimated plant. I didnt give up though, i have a beautiful highly varigated thai constellation, and a milk confetti that im trying again on. So far, so good, they've lasted just over the week mark so far. If you would ship to the USA, please let me know, I'd pay for the shipping. 😅😅😅😂😂😂
Oh nice work! Yeah, I feel you re: price of some of these things... I wish I could ship outside Aus but the laws are such a pain here that it's not worth it.
😀😀😀😀😀🤣🤣🤣🤣😂😂😂😂😃 GREAT JOB PETE
Hehe thanks mate
A bit of acidity is good for your skin. I never had any problems with 3% hydrogen peroxide. If it foes not hurt leaves and roots.... In the end it's just water and oxygen.
I think the reason it doesn't harm plants is because of their cell walls, but with other organisms it destroys the cell membranes.
I have been wanting to buy some tissue culture plants, but it’s so hard to know who is a trustworthy seller. Anyone with any recommendations? I am in the US.
I think you just have to try different places until you find someone reliable. That's what I've done at least.
@@petevsplants7516 thanks, I just gotta be brave and do it!
Kinda funny you mention other people freaking out about TC while you are freaking out about TC with 42 unnecessary steps for already-sterile plants! ;)
OMW I'm so jelly right now...I want 1 of these so bad but back in South Africa they are so overpriced. One day when I'm big I will get 1 😂
Yeah, they're insane almost everywhere
We can get an unacclimated monstera mint -tissue culture for $212.00.
Wow, nice!
I got mine for 200
@@Dieppaloveplants Wow, nice work!
💪🐻👍
:D
Shit load of bleach, literally! 😂
Leaving ANY agar scares me. I lost 7 of 10 aglionima pictums tc from the stems rotting off. I fully attribute it to not getting off all the residue.
My tc TC is doing fabulous! I was super anal about washing off 100% of the agar. Even used a baby toothbrush on the roots. I also inoculated the roots with mychcrorrizal and azospirillum.
I think dipping in copper and not rinsing makes sense. I’d be going for the residual of the fungicide.
If you rinse, you kinda did the exact same thing the hydrogen peroxide bath did.
Congratulations on the PSS! It would have taken me at least 30 min each cleaning those bad boys up. Especially as rare as they are in your area. Locally, the prices have pretty much collapsed to reasonable. You can get small rooted plants for ~$250. Seeing tc for double digits and even seeds….but…?
I did do a thing though! Rooted node with a sprouting leaf BMF inbound soon. Couldn’t pass up the chance @$125 shipped.
I enjoy growing lil things into big things and I should be way ahead of the game vs waiting for the price to continue to drop. (Even if that’s not true, that’s my story and I’m sticking to it!)
Yeah that's crazy re: prices in the US. BMF are still $2000-2500 per leaf for an established plant and a large PSS would be $5k+. TC should bring them down but it'll probably take another year or so. Everything's 1-2 years behind the rest of the world here in Aus because of our importation laws.
@@petevsplants7516 Just watched the unboxing video North Shore Tropicals did. Vancouver, Ca is about two hours north of me. Those small PSS and BMF she unboxed were priced at $228US. Super solid prices, especially for a nursery.
That's ridiculous... as I said in the comments, each of those would've been 10-20x that price in Aus. I saw a 1 leaf BMF the other day and the price was $2500 AUD
Were those philodendron spiritus sancti have varigated?
Haha no... those are about $20k Australian still. Definitely not in my price range.
@petevsplants7516 OK I thought I saw white I was thinking dang they got those in tc quick. There like 20k USD dream plant but definitely to much for me
Yeah, they're in TC but there're clearly not many of them around as the prices are still so insane.