CRISPR Cas9 Screening

Created by Shivani Baisiwala, BS, MS, MD Candidate 2021
This video provides an overview of setting up and conducting a large-scale CRISPR screen in the context of Glioblastoma (GBM). Although we focus on GBM here, the concepts are applicable to a wide range of scenarios. Our ongoing CRISPR work is performed as a collaboration between the Sonabend and Ahmed Labs at Northwestern Neurosurgery, supported by NIH/NINDS.
For a higher quality version - see expl.ai/WBLAHKC
Papers and figures referenced:
www.nature.com/articles/s4146...
www.cell.com/cell-reports/pdf...
www.computescotland.com/image...
www.cell.com/cell/pdf/S0092-8...
blog.addgene.org/plasmids-101...
www.thermofisher.com/us/en/ho...
www.axolbio.com/page/transfec...
www.mirusbio.com/applications...
pharmaceuticalintelligence.co...

[@ksn4911]

I was struggling to understand a paper related to CRISPR screening and this lecture was very helpful! Thank you!

[@ahmed-lab]

Great to hear!

[@dianaprincess9458]

Me too

[@ranxinzhang4446]

Great lecture! Love how she explained everything so clearly. Thank you!

[@ctrlaltdelicious6072]

Wow this was the most useful video on transfection/transduction/CRISPR I have ever seen and I am so thankful for content like this. I am preparing for a job interview :)

[@biggjones001]

I loved this totally! And a great voice to go with the explanation. Thanks

[@botan3020]

A Spectacular Video! I learned a lot! Thank you!

[@dianaprincess9458]

Thank you after I listened many videos, I couldn’t understand, I got very clear from this video.

[@vickyweechee]

Thank you! Very great explanation, you help me a lot on understanding this.

[@nirmalapun3945]

Amazing. Such a clear explanation. Thank you so much.

[@hinaldhaduk7506]

very informative, easy to understand. Appreciated for uploading it.

[@AnhVu_8891]

Thank you so much, OMG, I really appreciate your incredible lecture. It's very clear to understand about CRISPR-Cas 9.

[@us_visa_apply]

Thank you so much for such informative and easy-to-understand videos. Appreciate it a lot.

[@meryemsinemuyar6233]

the best explanation that I saw! Thank you

[@gokhanakman9785]

Very helpful and great explanation of the CRISPR Cas9 Screening!

[@selamibaglamis6007]

Great explanation, I learned so much. Thanks a lot :)

[@mengzhao7911]

Great explanation, I learned so much.

[@anjanitubati2193]

Thank you so much !! Great lecture with lot of details explained very neatly. Many doubts were cleared.

[@shawsheryl5092]

So clear! Thanks!

[@aysekaymaz6046]

Great explanation! Very helpful

[@naimehrafatian3946]

it is the best video of the technology I saw so far

[@salahadlat]

This lecture is very helpful. Thank you very much

[@liuliuqiu03]

very useful! Thank you!

[@DhamminiEkanayakeMudiyanselage]

Thank you very much a wonderful video

[@xchan6905]

Very useful and easy to understand.

[@saintlukman]

Very useful and simplified

[@George-rq1yp]

Excellent!

[@damialaoalao7137]

This was really good

[@mamiburgac2032]

First of all, thank you for your effort. I have a question about the numbers of the construct. Why there are 77,000 constructs, and how to determine these numbers? Could you basically explain, please?

[@EDYAJ353]

The human genome contains approximately 20 k genes (here they say 19 k), she also says that you want to have 4 sgRNAs for each gene just in case one doesn't work. So 19 k times 4 sgRNAs per gene is approximately 77k constructs. :)

[@josephpareti9156]

'ChatGPT for CRISPR’ creates new gene-editing tools - is this the way to go ?

[@asyaalnaseri7571]

Is that Kim Kardashian explaining

[@Sciencenerd2704]

Hahahaha