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The Ahmed Lab: Northwestern Neurosurgery
United States
Registrace 12. 05. 2020
The Ahmed Lab is a research laboratory at Northwestern Medicine's Department of Neurosurgery. We study glioblastoma, which is the most common type of adult malignant brain tumor. This channel is all about showing you what we do - from giving a snapshot of our everyday lives to creating teaching content on common skills and techniques in research. You can learn more about us at www.ahmed-lab.org or @ahmedlabnw (twitter).
Ahmed Lab 2021
The mission statement for the Ahmed Lab @ Northwestern Feinberg School of Medicine
zhlédnutí: 1 127
Video
GBM Research: An Educational Series
zhlédnutí 2,1KPřed 4 lety
Our channel focuses on providing high quality content about GBM research and broader molecular biology research topics. Subscribe for useful educational content!
Gene Modulation: Creating Knockdowns, Overexpressions, and Reporters
zhlédnutí 1,4KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a detailed overview of how to use plasmids to modulate gene expression in the lab. This technique is used for many research applications as it can help us directly link a gene expression change to a specific cell phenotype and can allow us to test novel gene therapies in the lab. We discuss here starting from a glycerol...
MTT Assay for Cell Viability
zhlédnutí 95KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a detailed overview of how to perform an MTT cell viability assays. We discuss the theory behind the assay, the potential pitfalls, the step-by-step procedure, and the analysis and interpretation of these assays. All research shown here is from the Ahmed Lab, supported by NIH/NINDS funding. Further protocols can be foun...
Flow Cytometry
zhlédnutí 2,3KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a basic introduction to understanding how flow cytometry works, how it can be interpreted, and some key considerations in how to perform flow experiments. We discuss the fluorophores available and the different graphs and gatings that must be performed to assess the data. All research shown here is from the Ahmed Lab, s...
Quantitative Polymerase Chain Reaction (qPCR)
zhlédnutí 27KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 Full protocols for all assays available at ahmed-lab.org This video provides an overview of the theory and practice of quantitative PCR, a widely used technique in molecular biology laboratories in order to measure the mRNA expression of genes or to measure activation of gene transcription for specific genes. Here, we go through both the t...
Immunofluorescence (IF), Immunohistochemistry (IHC), and Immunocytochemistry (ICC)
zhlédnutí 50KPřed 4 lety
Created by Shivani Baisiwala, BS, MS Full protocols available on our website www.ahmed-lab.org This video covers the common technique of IF, which is also known as IHC or ICC depending on the type of sample being studied. This is a common and powerful technique used to study protein localization and expression in cells and tissues. All materials in this video are supported by the Ahmed Lab at N...
Western Blot Protocol Overview
zhlédnutí 13KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD See protocols on www.ahmed-lab.org This video provides a step-by-step overview of the theory and practice of western blotting, which is a technique used in molecular biology to identify the levels of specific proteins of interest across multiple conditions. Here, we go through practical aspects of performing a blot but also discuss the reasoning behind e...
Introduction to Cell Culture
zhlédnutí 9KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 See protocols on www.ahmed-lab.org This video provides an overview of the steps involved in passaging cells in order to maintain, count, harvest or seed them for future experiments. This is useful for many molecular biology assays as cells form the basis and must be consistently maintained in order to do assays.
Welcome to Wet Lab: Pipetting & Other Basics
zhlédnutí 2,9KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides an introduction to some basic wet lab concepts for any molecular biology laboratory. It includes a discussion of some general rules of thumb in the lab, understanding the different types of trash, different tubes and tools available, and finally goes through how to set and use various types of pipettes in the lab.
Writing a Scientific Project Proposal
zhlédnutí 1,7KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This is a basic overview of how to write a project proposal. It is directed at a simple undergrad or early graduate proposal and gives an overview of key concepts to include. All work discussed or referenced in these videos is supported by the Ahmed Lab at Northwestern's Department of Neurosurgery and funded by NIH/NINDS grants.
RNASeq Overview
zhlédnutí 9KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video discusses a basic overview of RNA-Seq, explaining how it works and how it is typically analyzed and visualized. Any ongoing work presented here is performed by the Ahmed Lab at Northwestern University's Department of Neurosurgery, supported by NIH/NINDS.
Introduction to R
zhlédnutí 1,4KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video is intended as a basic introduction to performing analyses in R, directed at new users. All work shown here is performed at the Ahmed Lab at Northwestern Neurosurgery, funded by the NIH and NINDS.
Intro to Proteomics / Mass Spectrometry (MS)
zhlédnutí 65KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video covers the basics of how to setup and interpret a proteomics screen. All research in this video is performed at the Ahmed Lab at Northwestern's Department of Neurosurgery, and is supported by the NIH and NINDS.
Developing a Scientific Hypothesis
zhlédnutí 2,8KPřed 4 lety
Created by Shivani Baisiwala, BS, MS, MD candidate 2021 This video is meant as a basic introduction to how to think about developing a scientific question, creating a hypothesis, and validating that hypothesis. It also briefly goes into how to build up a project from a hypothesis and how to think about validating specific mechanisms. All work shown here is supported by the Ahmed Lab at Northwes...
Thank you so much ❤❤
Appreciate!
ı just really hate your voice the tone of your voice the way of your talking it just makes me sick
Thank you so much for your clear explanation 🙏. I have learnt quite a lot from you !
Thank you very much!!!!
'ChatGPT for CRISPR’ creates new gene-editing tools - is this the way to go ?
wow. This is clear to follow and understand RNAseq for beginers. I save the video
Thank you for making this helpful video : )
Thank you very much for your video! a small question: Is it possible to do the mRNA with a small sample size (e.g. 100,000 cells) for non-housekeeping genes?
This was wonderful. Very appreciated.
it is the best video of the technology I saw so far
you really really demystified RNA seq....super easy to understand....Thank you☺
Great explanation! Very helpful
Thanks alot
Wow this was the most useful video on transfection/transduction/CRISPR I have ever seen and I am so thankful for content like this. I am preparing for a job interview :)
I watched several videos on IHC, IFC, ICC. The is the best i have seen. This is succinct, practical, and coherent. This is excellent. I was a 60mins that felt like 5 mins. Thanks a lot for this masterpiece
Can MTT be used for virus quantification?
No. However, it can indirectly measure toxicity induced by virus infection.
Love how thorough this video is! Would you by chance consider another follow up video with immunohistochemistry dilution calculations? I'm in undergrad, and would love to know this before volunteering at a lab!
Thank you so much for such a great tutorial. Super helpful and easy to understand.
Thank you so much, good explaining ❤❤
i'm interested in the info but the vocal fry is making it hard to listen.
Thank you
So clear! Thanks!
I loved this totally! And a great voice to go with the explanation. Thanks
Such an amazing video!
Clearest explanation of ChIP-seq I have come across so far.
Thank you so much for such informative and easy-to-understand videos. Appreciate it a lot.
Very helpful. Thank you!
big help, thank youu!!
Best explanation! Thank you!
This is one of the best biochemical videos I have seen. Thank you so so so much.
Great content.
❤❤❤ exceptional
very well explained thnk you !
Hi! Thank you for such an amazing video. Can you please explain a little bit about the difference between monoclonal and polyclonal antibodies in terms of use in western blot?
The final concentration of MTT that you’ve used is 5ug/ml. Bt i guess it’s actually 0.2mg/ml - 0.5mg/ml. In our lab we’re routinely performing the assay and we use 0.5mg/ml concentration.
DU145 is a cancer cell line
Very confusing 😢
This was really good
I'm sure that its not her voice, which does give a cringe!!!!!
ikr? the topic is so interesting but the voice/tone is killing me
That was a splendid discussion. However, i was wondering if you do really enjoy your work, because your voice says otherwise.
This was a very helpful video! Where would you recommend purchasing the MTT assay, and would it include this protocol?
The lecture is wonderful
A fantasic video and explanation except that sickening vocal fry
Formaldehyde used for crosslinking is quenched by glycine..what does it mean exactly mam?
Quenching is inactivation of the molecule
Excellent as usual!
Excellent!
Thank you so much !! Great lecture with lot of details explained very neatly. Many doubts were cleared.
This video helps me to understand many important facts about Ab staining and to carry my IF test. Many many thanks for making and uploading this video for people like us. The way of presentation is very smooth and clear.
👍👍👍👍Excellent! Just what I was looking for.