How to Grow Mushrooms: Grain Spawn & liquid culture Jar Lids

This video shows how to make jar lids for mushroom cultivation. The lids consist of a rubber self-healing injection port and a syringe filter. They can be used for liquid culture, grain spawn, BRF cakes, no pour agar jars, etc. The lids are easy to make, reusable, and make the inoculation of grains or liquid cultures very easy. At the end of the video, I mention some alternative materials such as synthetic filter discs, micropore tape, cotton balls, polyfill stuffing, and using high-temperature silicone to make the injection ports. Some of these options may be better or worse than others. The benefit to some of the materials is that they are easy to get or are cheap.
Links:
- linktr.ee/VonMalegowskiRecomm...
Timestamps:
0:00 Introduction
0:57 Making The Lids
3:19 Alternative Materials
4:05 Outro
Equipment Links:
- Self Healing Rubber Injection Ports: amzn.to/3EkiTXd
- Syringe Filters 0.22μm: amzn.to/36aZETg
- Ball Mason Jar Lids (Regular): amzn.to/3KM7h1C
- Regular Mouth Mason Jars: amzn.to/3uG2ucA
- Micropore Tape: amzn.to/3jHtuSo
- Synthetic Filter Discs: amzn.to/3vcDPv8
- PolyFil: amzn.to/3LYuGwW
- Pressure Cooker: amzn.to/33X5pm2
- Sterile Disposable Scalpels: amzn.to/3mHFpS6
- 70% Isopropyl Alcohol: amzn.to/3mIGzwW
- Alcohol Prep Pads: amzn.to/3Hklxwh
- Mushroom Grow Bags: amzn.to/31cCWb2
Music by Karl Casey @ White Bat Audio
Tracks: Legend, Existence
Photos and Videos Creative Commons Attribution:
- How to make a Liquid Culture for Growing Mushroom Mycelium | Quickest way to make Liquid Culture, By: Mycotopia CC License (support.google.com/youtube/an...)
- Using My DIY Still Air Box, by DIY Mushroom Enthusiasts CC License (support.google.com/youtube/an...)
#Mushrooms #GrainSpawn #Mycology #VonMalegowski
©Von Malegowski. All Rights Reserved.

[@lucasgsa]

Great video! Really liked the synth aesthetic

[@judithpriest221]

Thank you for the easy to follow explanation. It is very much appreciated.

[@dashodelamacchia]

Useful and clear video, thank you a lot.

[@EnotikC]

very goodddd

[@nasirilelaboye2846]

Can pasteurize effectively remove contaminants from the grain

[@dashodelamacchia]

no, you need sterilization in a pressure cooker.

[@chrismichalak1046]

How long do the filters last? I.e how many times can them pressure cook them?

[@puffandstuff72]

Same question here...i was wondering if you can put those in a pressure cooker

[@BossyGerbasi]

You can just put foil over the lids, and make sure to loosen the lids so all the airflow isn't stressing the filter you put on the lids. ​@puffandstuff72

[@trevorbraden5448]

You don't need the silicone for the rubber injection ports or the filters.... if your holes are drilled the right size it's already a tight enough fit...... been doing lids without silicone for ever and never encountered a problem.... it's an unnecessary step

[@vonmalegowski]

Good point.

[@Lvaudioworks]

I recently heard that when spraying rubbing alcohol to sterilize, if the alcohol mists on the filter it can cause bacteria or damage the filter?? Have you had any issues with this?