Light-sheet microscopy

BOOKMARKS:
What is light-sheet microscopy? (0:13)
Advantages of light-sheet (2:38)
Requirements for light-sheet microscopy:
1) Question at the right scale (5:32)
2) Cleared sample (10:40)
Workflow for a light-sheet experiment in big, cleared samples (14:30)
Things to do BEFORE imaging (17:21)
1) Trim sample (17:29)
2) Pick a good clearing method (19:00)
3) Label with proper fluorophores (21:39)
4) Optimize mounting (23:37)
Thing to be aware of DURING imaging (27:53)
1) Types of problems (28:03)
2) Radial aberrations (28:55)
3) Light propagation (30:47)
4) Sheet shape (31:44)
5) Tiling (45:21)
6) Laser power and exposure (46:38)
Thing to be aware of AFTER imaging (47:24)
1) Data management (47:37)
2) Data visualization (50:36) *see Imaris Viewer Introduction Video:
3) Data analysis (51:54)