Pouring agar plates for mushroom cultivation
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- čas přidán 12. 07. 2024
- In this video we walk through a couple techniques to pour agar into sterile petri dishes. The petri dishes being used are standard 10mm x 60 mm plates and the agar being used is MEA for culturing mycelium. Gary Heferle is the owner and head mycologist at Fresh from the Farm Fungi in Denver, Colorado. Fresh from the Farm Fungi is a gourmet mushroom farm that strives to provide premium, high quality mushrooms to the markets in Denver. We also sell a variety of liquid cultures on ETSY:
Gary's Recommended Lab Gear: www.amazon.com/shop/influence...
www.etsy.com/shop/freshfungi
For more information visit freshfromthefarmfungi.com MUSHLOVE! - Věda a technologie
Just wanted to note that we usually use the smaller 6 mm plates for production and quality control, but these plates work just as well and as you can see are quite easy to pour! For more information visit www.freshfromthefarmfungi.com MUSHLOVE! #mushroomcultivation
Liked and subscribed. Thanks homie, I’m pouring plates tonight and you just gave me the reassurance I needed
Awesome hope they come out perfect! ❤️🍄
I feel like I'm earning my MushLuv diploma watching your vids.
Which plates do you buy for your culturing now? Can you tell me where you buy them?
Re: problems with corrosion using Bleach - How do you feel about quaternary ammonium products vs bleach? It's my understanding that it's non-corrosive and more effective than bleach. Love your channel - thanks.
keep the agar jars in the PC or cooking pot with a lid on(while it still has some heat), until you need to pour, this should help with clumping at the end.. although i almost always have some, but the warmer they are kept and the faster i move, the more completely i use the agar in the jar. i use dish washing rubber gloves because they are thicker and mitigate the jars heat.
i also pour vertical stacks and while i do get condensation, i usually leave them an hour or two in front of the flow hood, which cuts down significantly any condensation. i have no problems with the condensation in the dishes causing contamination or inhibiting the growth in the next phase for upwards of 7-8 weeks, although they get used in half that time.
Stack a hot agar jar on top of stack helps with condisate
Regarding allowing your plates stand for 72 hours in front of your flow hood as a QC measure: I can see the logic in this. But what if one were to pour in a SAB? Would it be better to let them sit, wrap them, or use ASAP?
let them sit with the lids closed, if you wrap them they may have condensation it will be ok but it’s annoying when you go to use them. You can use asap but if you get contam you won’t know if it was from pouring or lab work.
Is condensation a problem? And how do I avoid it
Condensation can be a problem when it pools on top of mycelium long term (which would cause it to get weak/die) or if it carries contaminants onto the agar which may happen if it is stored in less than ideal environments. There are ways to prevent contamination during pouring (cracking the lid in front of the laminar air flow, pouring when the agar is cooled significantly, stacking up plates during cooling) and also condensation can be addressed while storing plates by keeping them agar-side up in the refrigerator. Also, tapping moisture out carefully before using plates is a quick fix but can lead to contamination. MUSHLOVE
What did you do with the plated after they were poured parafilm them and store till later or did they stay in the hood till the class?
I usually keep them in the hood for 4-5 days to ensure sterility then use them up or store them in the fridge in the sterile sleeves that they come in until ready for use
@@FreshfromtheFarmFungi Silly question but does the hood stay on during these 4-5 days?
Im wondering the same thing. You just leave it on for days? Idk if my flow hood can handle that
What are your thoughts on using gentamicin in agar or LC?
My thoughts on antibiotic usage are conservative because of my previous clinical and laboratory experiences. Gentamicin is a broad spectrum antibiotic that would be useful in this procedure, and is common among growers but I prefer to use clean, and precise techniques to eliminate the need for antibiotics. This conservative opinion is because of potential environmental factors and the potential for masking imperfect techniques. Preventative measures are in place to prevent contamination, and using antibiotics masks any flaws in the system. Furthermore, when antibiotics are flushed down the drains, or discarded in waste, they accumulate in the environment and can produce antibiotic resistant bacteria. This is very relevant in the Great Lakes region of the US (Specifically on the shore lines of lake Erie) Where each year, large amounts of antibiotics that are used in agriculture, wash onto the beach and slowly help build a resistant population of bacteria in the sand and shores that would otherwise not exist. Hope this makes sense (and it's just my opinion) - MUSHLOVE
How do you make agar?
I use glas petri dishes and have some problems with contamination. Is it a good way to sterilize the petri dishes with agar?
You can sterilize them in a pouch first or foil and then pour them
@@FreshfromtheFarmFungi thx Gary but I have a few problems when I do it this way.
I want to sterilize the already poured petri dishes with MEA (or other agar receipe). I would use these special bags they use in hospital kitchen, they are autoclavable and seal themself.
The main problem ist that I want to pour 20x agar plates at once but only need a few. the rest gets contaminated to much
DO you have experience with this way? BR and thx - awesome channel :)
Why bleach instead of ISO?
Can Petri dishes be recycled?
I save my old ones and use them for spore prints - you can get glass ones that are re-usable but Im not sure if you can recycle
@@FreshfromtheFarmFungi thanks for response. And congrats on the amazing channel!
its not "auger" its ay-ger