Counting cells using a hemocytometer video protocol

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Watch our cell counting video protocol that takes you through the stages of preparing hemocytometer and cell suspension, counting the cells and checking them for viability.
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[@MNishCT77]

6:04 If my calculations are correct, if you have a viable cell count of 200,00 cells/mL in a total volume of 20 mL, then that should be 4,000,000 cells. If you then want to to seed 10,000 cells into the new flask (0.0025 or 0.25% of the total cells), you should transfer 0.05 mL (50 mcL) of the original sample into the new flask.

[@ashb6136]

totally agree

[@gurmanbrar8945]

Plz tell me
Total procedure of platelets counting

[@osmirod1]

Uhhhhhhhhh Abcam made a mistake

[@funny11744]

How to prepare a trypan solution 0.4% starting from powder 60% trypan blue ?

[@funny11744]

Wow, counting cell with inverter microscop. Please mention the type of hemocyometer ?

[@zhengyiyang5294]

czcams.com/video/GCohewzhD3k/video.html, For the 2nd fomular, are you sure you want to use count1 to divide by 4, not count5 divided by 4?

[@xaviervela3889]

I think is count5 divided by 4

[@jack7195]

For anybody stressing over the calculations at the end, these are wrong.

[@khurshaidanwar1580]

6.07/6:05: Formula= Desired number of cells to seed/Total number of cells=Volume to transfer/Volume of the cell suspension
10000/4000000=volume to transfer/20mL
10000/4000000*20=0.05mL which is equal to 50 microliter
Further
Total cells=4000000 and total volume is 20000ul so 1ul contains= 4000000/20000=200 which multiply by 50= 50*200=10000 cells