Agarose Gel Electrophoresis Tip #2: Adding Ethidium Bromide
Vložit
- čas přidán 6. 09. 2024
- To speed up your agarose gel electrophoresis, try adding ethidium bromide to your gel solution. NOTE: Ethidium bromide is a known mutagen. Be sure to use all necessary safety equipment when handling it in the lab. This includes a lab coat, safety glasses, and gloves.
For the full written Electrophoresis protocol, visit:
www.addgene.or...
Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!
I've found GelGreen to be the best. Stable for decades at room temperature, extremely sensitive, blue-light-excited so no worries of UV damage to DNA or UV safety, and it comes in an AQUEOUS solution, much more safe than other modern dyes dissolved in skin/glove-permeable DMSO.
And you can pick up perfectly compatible blue-light imager and enclosure from ioRodeo for only a few hundred bucks.
0:28 I don't know about the concentration, but I generally add about 10uL of EtBr to that volume. Then I let the flask cool a little bot longer (with a stirrer inside it). Then I pour it and allow the gels to solidify.
Kristofer Grove