Agarose Gel Electrophoresis Tip #2: Adding Ethidium Bromide

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  • čas přidán 6. 09. 2024
  • To speed up your agarose gel electrophoresis, try adding ethidium bromide to your gel solution. NOTE: Ethidium bromide is a known mutagen. Be sure to use all necessary safety equipment when handling it in the lab. This includes a lab coat, safety glasses, and gloves.
    For the full written Electrophoresis protocol, visit:
    www.addgene.or...

Komentáře • 4

  • @addgene
    @addgene  Před 2 lety

    Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

  • @ShyamBhakta
    @ShyamBhakta Před 5 lety +4

    I've found GelGreen to be the best. Stable for decades at room temperature, extremely sensitive, blue-light-excited so no worries of UV damage to DNA or UV safety, and it comes in an AQUEOUS solution, much more safe than other modern dyes dissolved in skin/glove-permeable DMSO.
    And you can pick up perfectly compatible blue-light imager and enclosure from ioRodeo for only a few hundred bucks.

  • @thefenerbahcesk4156
    @thefenerbahcesk4156 Před 5 lety

    0:28 I don't know about the concentration, but I generally add about 10uL of EtBr to that volume. Then I let the flask cool a little bot longer (with a stirrer inside it). Then I pour it and allow the gels to solidify.

  • @BarbaraMoore-s9y
    @BarbaraMoore-s9y Před 2 dny

    Kristofer Grove