How To Perform a Disk Diffusion Test (Modified Kirby-Bauer Test)

This video demonstrates how to perform a modified Kirby-Bauer, or disk diffusion test. This method is used for testing the susceptibility of bacteria to different antibiotics. This activity is included in Biotechnology: A Laboratory Skills Course, Bio-Rad Explorer's textbook.
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[@babusheikh6652]

Hi there, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?

[@BioRadEducation]

Hi, Babu, we recommend you contact the manufacturer of the equipment and supplies you are using for your work for recommendations.

[@babusheikh6652]

@@BioRadEducation thank you for your suggestion