Michaelis Menten equation

In enzyme kinetics, Michaelis-Menten equation is a mathematical equation that relates velocity of enzyme V0, maximum velocity Vmax and Km. It explains both, the first order kinetics and zero order kinetics of typical graph of enzymatic reaction. Michaelis Menten equation starts with assumption that, enzyme, substrate and ES complex are under equilibrium. This assumption is also known as Equilibrium assumption. On the other hand Briggs Haldane assumed that the concentration of ES complex remains constant through out enzymatic reaction. This assumption is also known as pseudo-steady-state-hypothesis. Both Michaelis Menten and Briggs Haldane assumptions can be used for the derivation of equation.

[@elmoatasmbilakakhiri7891]

Hello!
Just wanted to leave a quick comment thanking you for your excellent video. It is a godsend! I couldn't wrap my head around the topic until you explained it in such a simple and concise manner. Kudos to you!

[@mahtabyaghouti5446]

That is a very nice description, thank you so much!

[@ivanrubalcava08]

Maybe i'm just tired, but this explanation established no clarity for me. Were you just deriving various equations from the M.M. Equation??

[@sagarsrivastava8238]

Replay the video , it will give clarity . And think about the stuffs more and more , like how things are going on inside.

[@smnoni4330]

Yes you are right

[@shruti1258]

And my 1 like made it 100

[@lekanganambela5183]

You were just tired. Cause I'm also very tired right now, and it does seem like he's just deriving equations.😂

[@inkumbuchawewa8275]

​@@lekanganambela5183 😂

[@mushkanshaw9610]

This video is extremely helpful & so well explained ....😊Thank you sir

[@athiengdeng8347]

Thanks so much for your contribution to my progress 🙏🙏🙏

[@varshalala7879]

Really really easy explanation.. I was so lost before hearing this. Now I can understand very well!!

[@BilalKhan-mj3ic]

Hi

[@faizanpro355]

I don't get it no matter how hard I try

[@ravidhanavath1765]

thank you so much, for such a clear cut explanation

[@user-sh3zs3ip1h]

Thank you so much for teaching it in a really easy way. May God bless you! ...

[@rachael4775]

Thank you so much, I never understand this after taking biochem for a semester

[@maestro_2621]

This is the greatest explanation on Michaelis-Menten Equations ever! Thanks and be blessed for this simple plausible explanation it's just straight forward if you know what you're doing🎉❤

[@lordxenakius6920]

Thank you so much,your video was extremely helpful bro.

[@pius2440]

thanks so much for the video
really helped me a lot because now it makes alot of sense

[@hasankarimi8539]

Thank you for explaining so easily

[@rajniyadav8947]

hats off you sir making such a conceptual , easy video for us

[@dewdrop4693]

Wow..this is super helpful and you explained very well..❤

[@jakha1272]

why the M-M equation in the enzymatic reaction focuses on V0 (initial velocity)?

[@snejpu2508]

On the other hand, if the concentration of substrate is very low ([S]

[@mordecairugag9873]

when S is very low, V = 0 right? Or V = (Vmax/Km) x S, but S is very small so V must be very small as well, where the graph is almost linear when S is small, but gradually curved into plateau when S became big enough.

[@Skilled7105]

will a large or a small value of Vo have a greater impact on the rate of the reaction?

[@Sadiqa-oh6vj]

Made it so easy to understand 👍

[@yannik310]

Great video... not many other youtube channels for biochem out there, so keep it up!
***Heads up for American viewers: when "into" is said in the video I believe it means "multiply". In the U.S. we say the opposite, "into" is for division, so that might be confusing for some people

[@eileenhenryselby-smith9762]

Nearly all the English-speaking world would use 'into' to mean divide

[@binteaziz2054]

​@@eileenhenryselby-smith9762😮 into ka mtlb tu multiply hota h xd

[@lalayatem3758]

amazing, thank you! God bless you sir.

[@sarahmajin5358]

Great video, thank alot. Please what does Kcat stand for?

[@replied9115]

Thank you so much sir. It all makes sense to me now

[@richardshane456]

🇺🇸
As an electronic technician this looks very similar to an electron bias on a semiconductor silicon or a geranium substrate junction
I pause to see if there's any similarities between conduction by electron transport
Although I do realize it's a molecular system

[@kimberlyknox7283]

a lot of molecular reactions occur through electron exchange so i’d imagine the equations reflect the similarities

[@mordecairugag9873]

Very nice, everything about these are in the textbook but sometimes we need someone to read it for us in order to understand.

[@Ayomikunajose]

Thanks, this was really helpful

[@mohammedvalikarimwala4700]

The best explanation for this equation

[@Isaharif]

i AGREE

[@danirurajapaksha7990]

Thank you so much I wish you could be my professor one day ohhhhhhh I really appreciate this work. God bless you 🙏🙏🙏🙏

[@tulikarajput8871]

Thanks for ur teaching skill....❤️❤️

[@sarthaksinghrajput5462]

you destroyed it!
thanks a lot my guru

[@niarandall7010]

Amazing explanation, thanks.

[@haribladeo6686]

Really great buddy ....

[@UnwanaAbijah]

Thanks very much for this detailed explanation 😊

[@ayshafariatwinkle8717]

Excellent. Thanks Sir.

[@xanhchim768]

great video, thank you so much

[@richardshane456]

Thinking a bit longer what is the propagation? @ the electron proton particle level transfer?
Perhaps I'm looking at it all wrong at the enzyme level?
If someone could explain or point me in the direction where I might be able to fill in that Gap....
I would appreciate it
Thank you, a fascinating subject

[@richardshane456]

5:53
What is the constant Km referencing as a mathmatical construct? A process from reaction to and from equilibrium states ? Denoted as a Constant called Km?
Is this Km constant the reaction times (lay person terms to understand the process) mathmatically created to allow a formula to prove itself mathematically called the mechaelis menden equation?
Ty

[@quickbiochemistrybasics]

Thank you for your question.
Km is the ratio of the rate constant related to dissociation of ES complex (KR and Kcat) to the rate constant that forms the ES complex (KF).
Practically it is calculated as the substrate concentration at which velocity becomes half of Vmax.

[@achuanju7523]

Thnku sir it's so useful and helpful video I ever seen about this topic

[@jalanshimotisha4704]

Too good explained ..Thankyou sir!

[@BilalKhan-mj3ic]

Hi

[@nihalmuhammed6698]

Thankyou for this... 👍

[@samitiitr07]

Very good explanation

[@sudipkumarmukhi726]

Crystal clear explanation but you need a calm mind to get it at one go 🔥

[@junedraza7205]

Best way to explain

[@neethu3938]

Thank you sir it's very useful to me

[@rhinoceros4971]

thank you so much

[@sriparnadas1275]

I have a question?
How you can relate reaction rate with the substrate
concentration from michaelis-menten equation?
Please respond 🥺 urgent

[@UniqueBansal44]

Nice explanation

[@nikhilhumane5540]

Amazing video

[@abduCeNs6]

Thanks very much

[@ayaalhossini2944]

Brilliant..🤩

[@nikolozpapiashvili1241]

this man is a hero

[@dibyendusarkar3317]

Excellent 👌🏼

[@Hrishi2231]

Great explanation sir..tq

[@manny_k2988]

im so failing this semester. How do I create a MM curve from absorption that I found using a spectrophotometer

[@kuenzangom5955]

Using Excel solver and model fitting.

[@nwoadrenochromdealer6667]

Could answer that, but it is too late now I guess

[@manny_k2988]

@@nwoadrenochromdealer6667 only 2 years🤣

[@luisr.ruiseco2581]

For the velocity what is “d”?

[@malakemail]

what about second order and third order kinetics?

[@subratamukhopadhyay4253]

Pseudo steady state means??

[@Drikkerbadevand]

OK that's all fine but what do you use it for practically? What can you use it for? It's just math for the purpose of math. I imagine Km is interesting because it represents the concentration at which 1. order kinetics is relevant, and thus a linear regression is achieved.. Which I guess can be used to calculate backwards if you're trying to figure out the concentration of a an unknown, ie. a blood sample or other.

[@IceBlueLugia]

Your voice sounds exactly like my dad’s lol. Not sure if that’s a good thing or not

[@ribble6381]

He is your dad

[@caringbeats8265]

@@ribble6381 yes once you should check

[@Dr_108]

Call him DADDY

[@caringbeats8265]

@@Dr_108 fumny

[@rakshamukhia7970]

what is Kf Kr and Kcat?

[@arshiaamjad9220]

very good explanation

[@somashishsaha]

Excellent 👍👍

[@kishwarmushtaq1271]

Can you please tell me why ES breakdown =Kr(ES) +Kcat(ES)????? Instead of Kcat(EP)??

[@johnnjoroge146]

because it is breaking down by forming products and reforming the reactants

[@karigankeppel1760]

The break down of Kcat[ES] is due to the fact that the enzyme substrate complex will break down and form just enzyme and just product. so you wouldn't have EP because there is no enzyme product complex. the only thing that is experiencing "Breakdown" is the enzyme substrate complex, so [ES] will be the only part of that equation.

[@doctorofpharmacologytoxico9556]

Thank you

[@mohammadtorikh]

Bagus penjelasannya. Terima kasih kawan

[@Solana009]

Thanks❤️❤️

[@muspanisho]

This video can increase your confusion of this topic

[@user-bw2rd9ko9t]

What is Kf and Kr

[@conceptbiosomes9277]

The ratio of Kr/Kf should be [ES]/[E] [S] and not [E] [S]/[ES].

[@anonymous-user-2024]

Thank you for the channel. Can you please make a video about single displacement, double displacement and ping pong enzymatic reactions. If not, can you please direct me to a good source. Thank you in advance

[@vidyashetty3483]

What is Vo ?

[@mayrudin9437]

i love learning things from kermit the frog (jk this was very helpful thank you)

[@kumudborole3098]

Explained wonderfully 👍

[@burkenaz2719]

allah razı olsun abicim

[@tahabashir9405]

do medical students study this, its not in A level biology?

[@yshu99]

Why minus sign is kept in this equation ?? -du/dt ?? Why minus sign ??

[@josephbiggus6757]

because we spend the reactants thats why we use -
. we produce the products thats why we use +

[@Sankalanersankalan]

because the substrate is decreasing and the product is increasing.

[@11eric222]

crazy, how they know that before 100 years ago

[@florachrist9602]

Guys what is the kf and kr there

[@karigankeppel1760]

the rate constant for forward and reverse reaction of enzyme and substrate. It's more of a metaphorical value in this particular video, but it's used to measure rate constants throughout interaction and all go into calculating Km

[@lalitasharma6687]

This look exactly like Langmuir isotherm

[@AllyKennedy-nv7ow]

You are my champion. My love even.

[@mayurhedaoo5814]

good bro.... i mean very good thanks

[@ambercurrie1476]

EEEEEEEE ehhs EEEEE ehhhhs, love the video it helped so much but that muddled my brain so much hearing that repeatedly 🤣

[@lenamanukyan8848]

I understand nothing.

[@Malatacarlos]

Maybe am tired too maybe it's the laziness in his voice

[@noeyg]

I think at 2:45 it is important to understand the Kf[E][S]=Kr[ES] is referring to the assumption at equilibrium the RATE of formation of ES and dissociation of ES is equal. Kf[E][S] is the association constant (Kf) * Enzyme concentration at equilibrium [E]eq * Substrate concentration at equilibrium [S]eq. Same logic for Kr[ES]eq. That is how that equation came up.

[@user-mt6rb4dl8b]

わかりやすいけどむずいよおおおおお

[@ekankitamishra9438]

I got more confused after watching this

[@Nelpaul159]

Yeeeasssss....

[@IbrahimQanyare]

Iam confoced

[@JeffreyDujduz08]

i only hear re zero instead of v0

[@nviscallin3702]

This has no buisnes bejnb this complicsed. Exam in 6 days ready to off myself

[@anomeric01]

Wtf

[@divyeshlal1360]

i still don't understand, fuck sake :(

[@ewaluchter-wasylewska763]

I am enzymologii and chemist together. Terrible.

[@varshitakamal4301]

Thanks... For nothing

[@saideepu9999]

Haa

[@ewaluchter-wasylewska763]

You can not Say as you have dane. Please learn a bit of chemistry . Terrible to hear being chemist.

[@MadhanaGopalB]

Good. But, your accent is quite annoying.

[@nikitatandia4405]

Very nice explanation

[@raghadr9262]

Thanks a lot