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It is clear now that when the chromagen interacts with the antibody with an enzyme the chromagen dissolve and produces colorization in the entire glass. What I am thinking before during the interactions of chromagen to an antibody with an enzyme, the small portion only of the antibody will only produce colored light.
Best explanation I swear the doctor who give me the lecture don’t know what to do I red the lecture for for 6 Hours and didn’t understand what my doctor was saying While you explain it in few minutes You are the best♥️♥️♥️
Excellent. Your video explanations are very simple and easy to understand. Your method of teaching is quite impressive. Please upload more videos on other techniques as well. I subscribed. Your channel need more subscribers.
well the way you teaching is awesome great work buddy keep going..... i would like to add a question that after binding of antibodies to antigen ( specific non covalent reaction occurs between Ag-Ab i.e epitope and paratope respectively) the secondary enzyme linked antibodies binds to primary antibody of fc region but as far i know two antibodies cannot react and binds each other then what basic principle holds this situation?? looking forward to your reply thank you
As u said.. that antigens have epitopes... so when our antibody is injected in the lab animal, that antibody acts as antigen for that animal... so this antigen (our antibody) will also have epitopes on its surface including fc region also which are called isotypic epitopes... so lab animal can produce antibody aginst these epitopes
Sir abhi tak naye videos nahin aaye. Please upload as soon as possible, I'm eagerly waiting. Please upload haematology, serology, pathology, biochemistry, mol bio techniques.
Agar koi investigation me anti hiv antibodies detect karne ho. to investigators ke pass hiv antigens hote hain jo invitro synthesize kiye jate hain ya fir kisi source se purify kiye jate hain... kits main ye antigens company provide karti hain
Every molecule including antibody or enzyme can act as antigen when injected to lab animal. If we inject our antibodies to mice.. it will act as antigen
Thank you Sir! Very helpful that you included the case where the primary antibodies aren't specific to the antigens to see the comparison.
You explained the toughest part in simplest way.hats off sir
Thanx kyra. If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5)
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THANK YOU SO MUCH! Your video was fast, simple, and informational. I appreciate you.
The Best Ever Explanation One can Get. Precise
best ever explained...thank you for explaining it so well.
Your lectures contains more amount of data , your vocals are easy for us to understand ..... 💓
Thank you from Cameroon 🇨🇲❤️
A really precise and pedagogical explanation. Well done.
It is clear now that when the chromagen interacts with the antibody with an enzyme the chromagen dissolve and produces colorization in the entire glass. What I am thinking before during the interactions of chromagen to an antibody with an enzyme, the small portion only of the antibody will only produce colored light.
Excellent explanation/instruction. [Antibodies is misspelled at 6:15 on the video.]
best explaination and helped me before my presentation 😊
this topic wasn't even clear in wilson and walker but you explained it very well. thank you
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Best immunology video I hv ever seen. .great job ..😊😊😊
Best explanation I swear the doctor who give me the lecture don’t know what to do
I red the lecture for for 6 Hours and didn’t understand what my doctor was saying
While you explain it in few minutes
You are the best♥️♥️♥️
Most welcome
Very nice 👍🏼
Excellent. Your video explanations are very simple and easy to understand. Your method of teaching is quite impressive. Please upload more videos on other techniques as well. I subscribed. Your channel need more subscribers.
Thanx Mohit ... i am in the process of uploading
Amazing👍🏻
Assalamoalaikum. Brother! I enjoy your lecture very much. Please keep it up. May Allah livelong you. Thank you
Walekumasslam... most welcome
Best explanation. Thank you
Awesome
Excellent explanation with diagrams
Well presented... excellent..
Bro your teaching method is too good and perfect
Most welcome Joshwa .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
veryy hardworking masahaalh keep it up sir ap electrophoresis pa bhi video upload krya
Thanks for the guidance
tq so much sr..concept clear n easy explanation
You are brilliant ...Brooklyn continue. with new video
Thanx
Excellent explanation! Thank you
Very very helpful video 👏👏👏
It's well explained and it's really helpful. Thanks for the video and hope you can do more. LOVEYOURSELF ♥️
Nice presentation, very easy to understand. Thanks a lot.
Brilliant explanation 💚
Very clear discussion!! Thank U Sir:'>
Explained very well 😊
Well explained ..thank you.
best explanation
Very nice indirect Elisa lecture
THANK YOU SO MUCH THIS VIDEO WAS REALLY REALLY HELPRFUL ...... EXCELLENT
Most welcome
If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5)
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well the way you teaching is awesome great work buddy keep going.....
i would like to add a question that
after binding of antibodies to antigen ( specific non covalent reaction occurs between Ag-Ab i.e epitope and paratope respectively) the secondary enzyme linked antibodies binds to primary antibody of fc region but as far i know two antibodies cannot react and binds each other then what basic principle holds this situation??
looking forward to your reply thank you
As u said.. that antigens have epitopes... so when our antibody is injected in the lab animal, that antibody acts as antigen for that animal... so this antigen (our antibody) will also have epitopes on its surface including fc region also which are called isotypic epitopes... so lab animal can produce antibody aginst these epitopes
@@sajidmicrobiology thank you soo much buddy
Sir update more videos. It is very useful to understand with animation.
Sure
Really well explained
Thanx
You’re amazing! Thank you so much :)
Very nice explanation sir thanks
Too gooddd
Very well-explained! 😊 👍
Very well explained.
Thanku so much. Explanation is too good 😊
Vatsala sharma most welcome
Sir abhi tak naye videos nahin aaye. Please upload as soon as possible, I'm eagerly waiting. Please upload haematology, serology, pathology, biochemistry, mol bio techniques.
The sample we are putting in the titer after the coating buffer. Is the sample just the blood of the person?
Its not whole blood... it is serum most of the time
Well explained! Hats of to you sir.
Excellent explanation, thank you 👍😀
Thanx
Nice
What an explanation!
That was perfect ,tnx so much
Initially from where we have to take antigen...?
Which are attaching to microplate surface.
Yes
Ur not understood my question.
Ooo antigen kidarse aya
Artificially availability is there of antigen
Agar apko koi antibody detect karni hain to apke pass uss antibody ka specific antigen purified form me chahiye..
Agar koi investigation me anti hiv antibodies detect karne ho. to investigators ke pass hiv antigens hote hain jo invitro synthesize kiye jate hain ya fir kisi source se purify kiye jate hain... kits main ye antigens company provide karti hain
very nice video sir thanks 👌👌👌☺️☺️
Very good explanation
Great
Is it possible to do the opposite by putting the antibody bound to the polystyrene plate to determine the antigen concentration in the sample?
Yes
@@sajidmicrobiology ok thanks
💞👍💞
thank u so much.... you did a great job...😊
Most welcome
Why are we adding the acid when we already getting the color by substrate addition only
To stop the reaction
Good explanation thanks
This was so great
what would you say the ease and speed of the process is? how long does it take to perform indirect elisa?
Best ever
Very well explained. Tq sir
Most welcome vijayachandra .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
Excellent thanks so much 😘 sir
Most welcome
very nicely explained.. thank u sir.
Most welcome
Thanks a lot.
Good explanation
Just woaw
Sir the chromagen color dissolve inside the glass or produces colored light? Thanks
It dissolves
Thank u so much
Sir will you plz conduct lecture on how to take notes from textbooks
has secondary antibody gain antigen property after binding enzyme to bind primary antibody!!
Every molecule including antibody or enzyme can act as antigen when injected to lab animal. If we inject our antibodies to mice.. it will act as antigen
Well explained!.. Thanks.
Thank you for this
Thank you so much😊
thanks alot ❤
Tqq so much
Tysm sir
I subscribed.
difference between sandwich anf this one
Nyc sir
thank you so much this was very helpful 🤍🤍
Most welcome
Where is video of direct elsiaa?
Is it 4 generation or third
Thank you ☺☺
Thank you
Direct also plzz sir
Sure
@@sajidmicrobiology thankyou so much sir.
Awesome
Awesome