Gene Ontology Analysis: Unveiling the Function of Your Genes
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- čas přidán 21. 04. 2023
- Gene ontology analysis is a powerful tool that enables researchers to uncover the biological function of genes, and it has become an essential component of genomic data analysis. By using gene ontology analysis, researchers can gain insight into the role of specific genes in cellular processes, identify pathways involved in disease, and discover new therapeutic targets.
One popular software tool for gene ontology analysis is TBtool, which provides a user-friendly interface for analyzing RNA sequencing data. RNA sequencing data provides information on the genes that are actively transcribed in a particular sample, making it a valuable resource for gene ontology analysis. TBtool allows users to easily perform differential gene expression analysis and pathway analysis, and to visualize the results using a variety of visualization tools, including heatmaps, volcano plots, and bar charts.
How to do Gene ontology analysis through tbtool?
Go analysis in tbtool?
To perform gene ontology analysis with TBtool, users first need to prepare their RNA sequencing data by aligning the reads to a reference genome and quantifying gene expression levels. Once the data is processed, users can import it into TBtool and perform differential gene expression analysis to identify genes that are differentially expressed between two or more conditions. TBtool also provides tools for gene set enrichment analysis, which enables users to identify pathways that are significantly enriched in the differentially expressed gene set.
In addition to gene ontology analysis, TBtool also provides tools for performing functional annotation of genes, including identifying conserved domains and motifs, predicting microRNA targets, and identifying protein-protein interaction networks. These additional tools can provide valuable insights into the biological function of genes and help researchers develop hypotheses about the underlying molecular mechanisms involved in specific cellular processes.
Overall, gene ontology analysis is a powerful tool for understanding the function of genes and pathways involved in biological processes. When combined with RNA sequencing data analysis tools like TBtool, researchers can gain deep insights into the mechanisms underlying disease and develop new therapeutic targets. By using these powerful bioinformatics tools, researchers can unlock the potential of genomic data and drive innovation in the field of biomedical research.
#Gene_ontology
#tbtool
#RNA_Seq - Věda a technologie
Hello, when you completed the analysis and open the output file, it was empty, then you magically fix it, can you show us how? minute 10:13. Thanks!
I have made the another video
Not able to get any data my excel file appears empty
After preparing file... When I go to bar plot my graph is not appearing only names of class(cellular, biological etc) appears.. nothing more papers..how to solve it?
May be you missed some point in the input file. Please your input files carefully
Good day, everything is with you?
How do I get the information so I can build the analyses?
I have made the number of videos that how you can get the information's like GO from RNA Seq data, or GO IDs from Protein Seq or GO Ids from other sources
You can find the video with title how to find the GO IDs Genome wide study.
Sir how's this file can be downloaded from which you have taken the data to excel ?
How to get the expression data from GEO.. watch this video or how to get GO IDs.
Please make a video how can we downolad these two RNA file for GO analysis
Video is already available dear. One can get from GEO expression analysis from NCBI and other is your gene list
@@genomewidestudy Please sir provide a link.
@@genomewidestudy wo video sy file agai ha pr ye file to totally different ha us file sy??
Sir i unable to get rna seq file kindly tell me
From where you want to get the RNA Seq File?
@@genomewidestudy could u please share ur mail there i can tell my problem more better.
You can tell your problem here, no matter or can join my page on facebook with same name
Sir could you please tell that why I am getting 1kb file with no information
Same problem
Kundan if you get the solution by any means please let me know
Ok I will check
Please save the file in Tab delimited format.
and then try. It will work
Hello sir....it not working...could you help ?
What is not working
@@genomewidestudy I'm getting 1kb files with no information
czcams.com/video/6QAUTrVZqEg/video.html
Watch this video, it is the second tool for GO
Also uploaded the video that what is the common mistake during GO analysis in tbtool. Please have a look
czcams.com/video/Ptj30Pj1j2A/video.html
thanks
Thanks for comment
you should clear the problem why students are getting 1 kb output file. Updating the software, or saving into delimited format dont help. Its important, cause people invest time in your video to learn, I hope you underestand the frustration
Thanks for your suggestion and comment. If you you are failed then try another method, which already in the video list.
I have made the video which is about the mistake during GO analysis in tbtool.
czcams.com/video/Ptj30Pj1j2A/video.html
lame