This is absolutely the BEST, MOST CLEAR, STRAIGHTFORWARD VIDEO I have ever had the pleasure of learning from! Thank you for making my day! College science should ALL be done with videos like this one
Thank youuuu! I missed a biochemistry lab and I couldn't have done my practical exam without understanding this fully. Your explanations are so easy to follow. My lab book is covered in notes now :)
I was going to wait for the video to at least get 1/3rd of the way before I was going to like it or not, but the Standard Curve definition and example with the consistently-themed penguin objects made me lol and informed, you deserve a like/sub!
Literally I watched and asked so many videos and people about this topic but still there are some lingering questions in mind. But this video cleared everything within 7 min. Tqsm
Thank you so much, I had to use a standard curve for something in uni and I had no idea what I was doing haha This is so clear and I finally understand it!
Thank you so much! You did an amazing job of breaking it down...I've been in Molecular Biology class for a few weeks and during lab, the professor didn't bother to describe what a standard curve was. I was thinking, "where is the curve?!". I get it now, thanks to you.
Excellent - clear, concise and educational! 2 questions, 1) How do you know what wavelength to use? 2) What is the difference between a spectrophotometer and a ELISA reader?
Thank so much ma'am....that's topic make me so difficult to understand because already miss lectures by professor but now I totally got understood What exact standard curve is.....thank you again
WOW! Thank you from the UK! My assignment is based on protein concentration in samples. This video explained it perfectly. I was clueless before I watched it! THANK YOU!!!!!
I just fell in love with you. You just made it so easy. I spent all day trying to understand the graph of ELISA, but I was stuck on standard thing. Now I've got it thanks to you :D
It depends on the solution, [FeSCN]^2+ for example is a reddish color solution, while Fe^3+ and SCN^- are essentially colorless. Since FeSCN^2+ reflects red light it absorbs BLUE light, so you find a wavelength in the blue light region! (I know this is a 5-year-old comment, but I just came across this video, hope this helps anyone!)
Could you also just dilute the sample 1:2 and then calculate the concentration? And thank you so much for the video!!! I finally understand clearly how it works!
Glad I came across your video I now have a better understanding of this procedure. But as an insipiring analytical chemist, can you ever detect 0 ng of "stuff" or whatever is being sampled?
Hi. The best explanation so far. But I have a question. What happens if the concentration that you got is above the maximum concentration? should you accept or not? and What must you do so the concentration must be within the calibration curve? I hope you'll reply
In less than 7 minutes, you've explained everything in lecture I zoned out on, omg thank you!!
This is absolutely the BEST, MOST CLEAR, STRAIGHTFORWARD VIDEO I have ever had the pleasure of learning from!
Thank you for making my day! College science should ALL be done with videos like this one
I'm so glad you found it useful!
instablaster
Thank youuuu! I missed a biochemistry lab and I couldn't have done my practical exam without understanding this fully. Your explanations are so easy to follow. My lab book is covered in notes now :)
+Francesca Hewitt I'm SO HAPPY!!! Thanks for watching and letting me know!
Kathryn Francesca what your University specialization??
I dont understand this curve in medical lab
I was going to wait for the video to at least get 1/3rd of the way before I was going to like it or not, but the Standard Curve definition and example with the consistently-themed penguin objects made me lol and informed, you deserve a like/sub!
I'm so glad to hear it - thanks so much!
this was generally one of the best videos I've ever come across, thank you so much!
Thanks so much! Glad it was helpful.
6 years later and it's still a helpful vid , thanks miss u just helped me pass my medical interventions exam
SO happy to hear that! CONGRATULATIONS!!!
Simple yet powerful explanation on stamdard curve and dealing with out of range samples.
Such clarity, that too in one shot🙏 wonderfully explained, Mam
Thanks 🌸
So kind of you to say! Glad it was helpful!
Literally I watched and asked so many videos and people about this topic but still there are some lingering questions in mind. But this video cleared everything within 7 min. Tqsm
Oh thanks! Definitely helped me to understand for my biochemistry lab report!!
OMG, I've struggled my whole life to understand this and you just made it so easy and simple. Thank you!!
Yaaaassss!!!!!
Thank you so much, I had to use a standard curve for something in uni and I had no idea what I was doing haha
This is so clear and I finally understand it!
Thank you so much! You did an amazing job of breaking it down...I've been in Molecular Biology class for a few weeks and during lab, the professor didn't bother to describe what a standard curve was. I was thinking, "where is the curve?!". I get it now, thanks to you.
THE BEST AND CLEAR demonstration
Thank you so much! Glad it was helpful.
thank you so much doctor
this video was so smooth to the brain
Smooth on the brain is a great compliment! Thanks!
you're so kind i was desperately looking for this. thank you so soooo much!!!!
I'm so glad you found me! You are sooooo welcome!
I have been struggling to find the values watched so much stuff but youu explained it soo smoothly god bless uh🌈⭐
Omg thank you so much for this knowledge you saved my academic life
Wow!!! You are so welcome!
Excellent - clear, concise and educational! 2 questions, 1) How do you know what wavelength to use? 2) What is the difference between a spectrophotometer and a ELISA reader?
Fantastic and simple explanation for those of us who are assay beginners!
Nice and clear explanation! Thanks!
Glad it was helpful!
That was extreeeeeeeemly helpful
I am soooooooo happy to hear that!!!
Thank so much ma'am....that's topic make me so difficult to understand because already miss lectures by professor but now I totally got understood What exact standard curve is.....thank you again
WOW! Thank you from the UK! My assignment is based on protein concentration in samples. This video explained it perfectly. I was clueless before I watched it! THANK YOU!!!!!
Thank you, you explained very clearly.....
Loved your explanation! Didn't have slightest idea in class what the standard curve is... Thank you!
BIOTECH TEST TOMORROW AND I AM SO READY THANKS TO YOU!!!
+Saul De Los Santos So glad to hear it! GOOD LUCK!!!
fantastic video!!
Excellent video, i had made lot of mistake and extended the x axis and y axis . but you have discussed it very clearly.thank u so much
Glad it helped!
My online course is using this video as learning tool. Thanks :) Your video really helped.
couldn't understand when my teacher taught me, but I got it first try when you did, thank you!!!!!!!
Your video is amazing.
Thank you! Glad it was helpful.
You are absolutely amazing!! God bless u!!
Wow!!! no one can explain better than you!!! awesome presentation!!! Thank you so much!!
Fantastic video, I understood everything perfectly. The ruler example made it all click in my head, thank you so much.
You're very welcome! I'm so glad it clicked.
Great great video, so much easier to understand
Glad you think so!
Or you can perform single/serial dilution, so that the unknown concentration falls within the 'ruler'?
You are the best!!! This is a really good contribution.
Thanks for watching!
Tbh.. this is the best video for explaining this.. finally I understood what's going on
I'm so happy to hear that! Thanks for letting me know!
You really make it easy to understand, thanks a bunch.
+Po Ho You are so welcome - thanks for watching!
Thank you! So easy to understand my assignment now! LIFE SAVING work done here today. Thank you Penguin Prof!
Glad it helped! You are most welcome!
This is very clear, thank you.
Glad it was helpful!
I just fell in love with you. You just made it so easy. I spent all day trying to understand the graph of ELISA, but I was stuck on standard thing. Now I've got it thanks to you :D
I love you too! Glad it helped. You realize you're dating a penguin now. Just to be clear. :)
+ThePenguinProf I'm so glad I am 😁
ELISA seems complicated but not at all! Thank you ThePenguinProf!
Kisses back! Good luck to you!!!
Oh god. I needed that! Wonderful
SO glad you found it helpful!
thanks very well explained, you must REALLY love penguins. haha
Thankyou you're a saviour ❤
So happy it was helpful! 🐧
Thanks. It made it easier for me to understand it.
I will definitely subscribe. You are amazing explaining. Thank you so much.
Thank you so much from Africa, i just subscribed. That biochem lab won't know what hit it.
wonderful explanation,
So glad it was helpful!
you cleared all my doubt in just 5 mins ..thank you
How do you know which wavelength to use?
It depends on the solution, [FeSCN]^2+ for example is a reddish color solution, while Fe^3+ and SCN^- are essentially colorless. Since FeSCN^2+ reflects red light it absorbs BLUE light, so you find a wavelength in the blue light region!
(I know this is a 5-year-old comment, but I just came across this video, hope this helps anyone!)
Really good brush up lesson, cheers :D
Thanks a lot. Really helpful.
May god bless you.
You are most welcome! Thanks for watching!!!
I love this video i ask for more: coefficients of variance. And controls meaning vs calibrators please.
Really great video I didn't understand it in class and this video was so helpful and easy to understand. Thanks so much!!!
So clear and simple! thank you
Thanks maam , it really helpful , if the profs in our school can do the same lecture as u did , i think all of us will love to study in this course!!!
You've just made my life a bit easier. thank you
Woo hoo!!! Thanks for letting me know!
Great Video! Tkank you so much! Best regards from student from Poland ahhaha!
I'm so glad it helped! Thanks for watching!
It helped me a lot. Thanks 🙏
Glad it helped!
omg you just helped me tremendously in my QBM class. Total ah-ha moment. thank you
I LOVE when that happens (and when people tell me about it!) Glad it helped!
thank you for your effort , its very simple and clear
Best explanation
Glad you think so!
So enlightened
So happy to hear it!
Could you also just dilute the sample 1:2 and then calculate the concentration?
And thank you so much for the video!!! I finally understand clearly how it works!
It's a complex relationship, so don't dilute and calculate... dilute and measure the ABS. Be safe. Glad it was helpful!
Thank you so much for your detailed lecture.. I just loved it. It provides all basic answers of mine about this topic.
Good luck 😊
Ahhhh. I understand this now. I didn't in my class. Thank you very much.
thank you....easy to understand and very helpful
Made that look easy ☺️
straight to the point thank you!
very helpful and very cute penguins!! thanks so much!
+Abby Jutilo You are so welcome! Penguins make everything easier, right?
Thanks a lot! You explained it better than my professor🤙
Happy to help!
Excellent advice and methodology for making any measurement. Thanks!
this is amazing! thank you so much
You're so welcome!
Terrific, terrific...thank you.
thank you so much, it is very helpful
I'm so glad! You are so welcome.
Glad I came across your video I now have a better understanding of this procedure. But as an insipiring analytical chemist, can you ever detect 0 ng of "stuff" or whatever is being sampled?
very well expalined.thank you.please make a video on TPC protocol and calculation
Nice explanation
Thanks!
Thanks!
Thank you, this is very informative
Thank you so much! I really needed this clarity!!!!
You are so welcome! Thanks for watching!
Thanks you made it so much simpler than my professor did!!!!
Nice presentation I really impressed good work thank you mam
Thank you for watching! Glad it was helpful.
Thank you so much,
Hi. The best explanation so far. But I have a question. What happens if the concentration that you got is above the maximum concentration? should you accept or not? and What must you do so the concentration must be within the calibration curve? I hope you'll reply
If your unknown concentration is above the standard curve, you need to make new (higher concentration) standards -- never extrapolate!
AMAGAAAAAA THANK YOU SO MUCH
for the longest time I felt so intellectually impaired but this video helped me loads
Thank you is the most that I can say tbh! what an amazing video!
Wow, thank you!
Thank you so much
You are amazing!!!
Very Helpful!
it was really clear
Thanks!!
Thank you Profpenguin!
Amazing! More videos like this please!
I loved this - well done!
Very helpful! Thank you so much!
You're very welcome!
Thanks you so much that helped so much.
Legend! Thank you! So easy to follow and understand....