This tutorial guides students on how to 1.) analyze a 16S rRNA gene sequence using Finch TV and 2.) determine the identity of their microbial isolates using NCBI BLAST.
Hi Burak! It sounds like you got a lot of BLAST hits that had high similarities. It means that's the resolution you are able to get with your sequenced product so really it would be difficult to choose. To narrow down further, you can sequence more of the gene you used to include more divergent regions. Alternatively, you can try sequencing other genes in your organism. Good luck!
I am getting dark grey vertical bars above those A,T,G and Cs, what those indicates? Some bars are of short height,some are small, on some its not there.
Hi shweta gehlout. The height of the bars you're referring to indicates the quality of the base calls. The higher the bars, the more confidence you have in the accuracy of the base calls.
dont know if anyone gives a damn but if you guys are bored like me atm you can watch all the latest series on Instaflixxer. Have been watching with my gf for the last couple of months xD
Hi Mr. Daren, I want to know the genotype of my SNP so I can calculate the correlation by SNP stats. What should I do please help me
Thanks teacher
thank you
Thank you!
Thank you so much. But i want to ask you something. I got many 97% similarities. I don't know which one to choose, what can I do?
Hi Burak! It sounds like you got a lot of BLAST hits that had high similarities. It means that's the resolution you are able to get with your sequenced product so really it would be difficult to choose. To narrow down further, you can sequence more of the gene you used to include more divergent regions. Alternatively, you can try sequencing other genes in your organism. Good luck!
Phylogenies!
I am getting dark grey vertical bars above those A,T,G and Cs, what those indicates? Some bars are of short height,some are small, on some its not there.
Hi shweta gehlout. The height of the bars you're referring to indicates the quality of the base calls. The higher the bars, the more confidence you have in the accuracy of the base calls.
I got it ...thank you so much ...😊
dont know if anyone gives a damn but if you guys are bored like me atm you can watch all the latest series on Instaflixxer. Have been watching with my gf for the last couple of months xD
@Luis Leonard yea, have been using instaflixxer for since december myself :)
Hello can I ask if I can download Finch TV in my cellphone?
I have not found a version for mobile devices. Sorry!