How to decide pH of the mobile phase | HPLC mobile phase preparation | voice of kayani

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  • čas přidán 3. 07. 2024
  • How to decide pH of the mobile phase | HPLC mobile phase preparation | voice of kayani
    This video based on HPLC mobile phase pH.
    in this video explained how to decide pH for HPLC mobile phase?
    how to set pH of the mobile phase discussed in this video.
    in this video also explained the relationship between pH and pka value of the analyte.
    Your quires;
    HPLC
    mobile phase
    pH
    mobile phase pH
    pka
    pka value
    pH vs pka
    how to decide pH for mobile phase.
    HPLC troubleshooting
    troubleshooting
    pharmaceutical industry
    chromatography
    interview questions
    QC Department interview questions
    HPLC troubleshooting interview questions
    voice of kayani
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Komentáře • 18

  • @SAURAVKUMAR-zx2il
    @SAURAVKUMAR-zx2il Před 7 dny +1

    Please make a Video on Related Substances Interview Questions

  • @pad480
    @pad480 Před 8 dny +1

    Hplc method development per video

  • @pad480
    @pad480 Před 8 dny +1

    Acidic pH ke liye mostly opa hi Q use kiya jata hai

    • @voiceofkayani5419
      @voiceofkayani5419  Před 8 dny +1

      HCl bhi use hota . Depending on mobile phase composition...
      But phosphoric acid is having high acidity, having strong acidic nature,that's why used mostly

  • @ashoksarkar7951
    @ashoksarkar7951 Před 8 dny +1

    Sir tablet bland sample kitna lena chahiye

    • @voiceofkayani5419
      @voiceofkayani5419  Před 8 dny

      As per standard concentration to make same ppm solution with standard... should be in limit of detection

  • @vasudevsharma-ey6ni
    @vasudevsharma-ey6ni Před 8 dny +1

    Sir API sampling , excipient( non API ) sampling ka rule same hai ? Aur guideline pls

  • @vasudevsharma-ey6ni
    @vasudevsharma-ey6ni Před 8 dny +1

    Sir ye impurity factor kya hota hai ?

    • @voiceofkayani5419
      @voiceofkayani5419  Před 8 dny

      @@vasudevsharma-ey6ni related to impurities calculation

    • @vasudevsharma-ey6ni
      @vasudevsharma-ey6ni Před 8 dny +1

      @@voiceofkayani5419 kaise ? Iski importance , use

    • @voiceofkayani5419
      @voiceofkayani5419  Před 8 dny

      @vasudevsharma-ey6ni dear,
      Pharmaceutical raw material Mai Jo impurities hoti Hain unka calculate Karna k impurity factor se related hai....iski bhot importance hai. Ye pharmacopeial hai material ki pure hona daikhna hota hai...
      Inko related substance bhi kehtai Hain....
      Is pe Meri videos available Hain plz watch on my channel

    • @vasudevsharma-ey6ni
      @vasudevsharma-ey6ni Před 5 dny

      @@voiceofkayani5419 sir impurity use karne se phle kya validation hota hai uska ?