BCA Protein Assay Reagent (bicinchoninic acid)

Learn more about the BCA assay: www.piercenet.c....
Accurately measure the total protein concentration in your sample with this two-component, high-precision, detergent-compatible assay reagent set.

[@ShakespeareCafe]

Safety Note: 0:46 To safely open the BSA glass ampule without cutting your finger use a 1000 microliter (1 ml) pipette tip which fits perfectly over the ampule) then break off the top without slicing your finger (I've seen it happen)

[@atosthewestie6768]

Thank you ! This is a super usefull advice !!! Have a good day

[@luketarosluck1601]

safety note? more like a pro tip! thx!

[@luisp.9710]

Hello.
I was going to use the Pierce BCA Protein Assay Kit which includes Albumin Standard Ampules, (2 mg/mL, 10 x 1 mL). The ampules are stored at room temperature (as suggested by the data sheet). Upon receiving the kit, i do not pay enough attention to the ampules, however, a few days later, when i was gonna use it, i was able to see some particles (like fluff). Ampules have not been opened yet
Are those particles fungus , BSA crystals or some precipitate of the compounds?.
The ampules contains BSA 2 mg/mL in 0.9% saline and 0.05% sodium azide.
I also looked up for certificate of analysis and says the following: " visual: Clear, colorless to slightly yellow liquid."
Certificates of Analysis of BSA Pre-Diluted Set that is sterile filtered (other product from the same company) says " viual: Clear, colorless to slightly yellow liquid, FREE PARTICLES"
Is my commercial BSA solution contaminated or normal particles?. Available from: www.researchgate.net/post/Is_my_commercial_BSA_solution_contaminated_or_normal_particles [accessed May 12, 2015].

[@margaretdorothy8111]

Luis P.
In our place they are stored at 4 degree in refrigerator.then just before use they are allowed to come to room temperature n we use.

[@JoettaPatch-b8t]

Alexandrea Street

[@madvi_singh]

I did that. And I was getting overflow result. What this happening

[@LawrenceLadouceur-s1d]

Deron Groves

[@jihadforgood]

Please can you tell me what is in the blank is it working reagent and water or working reagent only or water only?

[@luisp.9710]

Blank is the buffer in which your proteins are diluted. In my case I extracted my protein from cell with RIPA buffer, so only RIPA buffer is my blank

[@hafizmuhammadshahid1629]

Bicinchronic basic uses