How does a DNA sequencing machine work?

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  • čas přidán 23. 02. 2020
  • We discover how DNA sequencing machines read genomes with the Ramaciotti Centre for Genomics Deputy Director Dr Helen Speirs at UNSW Sydney.
    Videographer: Michael Kosmider
    Producer: Caroline Tang
    Stencil animation: Ralph Stevenson
    Cluster generation animation: Illumina
    For more videos like this subscribe to our channel: czcams.com/users/unsw?sub_...
    We're the official channel of UNSW Sydney, a brilliantly located university between the coast and the city.

Komentáře • 67

  • @rogeliozunigachavez161
    @rogeliozunigachavez161 Před 2 lety +38

    did you know that the first human genome sequencing in 2003 took about 13 years to complete and used about 2 billion dollars in funding? they also needed the equipment of several labs around the world because 1 single machine could not handle it... and now pocket sequencing devices.. isn't it just incredible?!

    • @sanjaymunroe9587
      @sanjaymunroe9587 Před 2 lety +1

      What's the name of the company that makes this machine?

    • @rogeliozunigachavez161
      @rogeliozunigachavez161 Před 2 lety +3

      @@sanjaymunroe9587 Oxford nanopore technology

    • @auwalushehu3316
      @auwalushehu3316 Před 2 lety +1

      Very incredible indeed

    • @itsomegali5342
      @itsomegali5342 Před 2 lety +1

      honestly i always considered the people who pioneered the science fields as the incredible ones.

    • @osmomosis9216
      @osmomosis9216 Před rokem

      @@itsomegali5342 everyone who made an impact to the field is incredible and worthy of respect. not just those who paved the way although they deserve their bit of recognition too

  • @poetry7260
    @poetry7260 Před 4 lety +25

    Overwhelming information but extremly interesting. Tech is moving us so fast into the future.

  • @colinbenson8337
    @colinbenson8337 Před 2 lety +7

    Would like to see more about DNA collecting, testing principles and methods, and testing, and producing resulting data.
    For seven years, in the late 1990s and early 2000s, after long careers in communications and electronics, I was a computer technician with tertiary photographic science and computing qualifications at a research institute for the sugar industry.
    With massive changes on the horizon, I discussed with a scientist, who effectively obtainied the DNA of sugar for marketing, the possibility of expanding to human and other DNA testing (such as sugar cane plants).
    DNA was then being highly promoted with CSI TV series.
    Personal DNA and crime scene investigations have since proliferated.
    A local murder in 2013 has become a cold case after a well-known journalist recently demonstrated that DNA testing had been overlooked and/or faulty. That could have been solved, locally!
    When I went to the CEO and suggested the institute expand its horizons to other fields, as with another suggestion to extend research to tropical fruit wines, for the institute's viability, the response was a definite negative. (I think he was more interested in fishing and impending retirement!) (The buildings of tge former institute that opened in the late 1940s and closed in 2005, are now a private college!)
    Now in my sunset years, if I had my life over, as well as having careers in radio, electronics, computing and teaching, I'd consider forensic science. Hence a quest for in-depth knowledge of the DNA testing process... and to encourage youngsters to consider that career.

  • @fifita3525
    @fifita3525 Před 3 lety +1

    Oh m'y god... This is amazing

  • @laynewyman3160
    @laynewyman3160 Před 3 lety +10

    The interesting thing is I figured out how to clone a dinosaur but I can't figure out genetic engineering.

  • @roscopico7817
    @roscopico7817 Před 3 lety +2

    Excellent, how do I find out the genetics of pigeons.

  • @gooberclown
    @gooberclown Před 3 lety +1

    Does this portable sequencing technology mean that I could purchase a carry sequencer and actually use it to check my own DNA SEQUENCE(S) from tissue samples of my own blood, saliva and hair?

  • @khangpilasangtam9254
    @khangpilasangtam9254 Před 3 lety +15

    Could you please make another video on how the Oxford nanopore technology minion works. And how accurate is the result. Does it gives result as accurate as other sequencing instruments that we use in the lab?

  • @mattgraves3709
    @mattgraves3709 Před 3 lety

    Amazing

  • @AbdolazimHasseli
    @AbdolazimHasseli Před 6 měsíci

    What is the name of this pocket sequencer?

  • @sanjaymunroe9587
    @sanjaymunroe9587 Před 2 lety

    What's the name of the company that makes these machines?

  • @Meleeman011
    @Meleeman011 Před 2 lety +1

    HOLY shit the future is now, I want that nanopore
    sequencer

  • @Raul28153
    @Raul28153 Před 2 lety +2

    Question: Using the Nova Seq, If you had a sample of DNA from a formerly unknown terrestrial species, would you be able to decode the various individual sequences strands as to their function and characteristics?

    • @indiabiologyexplains5013
      @indiabiologyexplains5013 Před 2 lety +1

      It would take quite a bit of work and the sequencer alone would not be able to do it. They would have to model the proteins from the genes, and then determine the characteristics of the organism. Still, the results would not be 100 percent accurate because the placement of cells(for a multicellular organism) cannot be told by the genome alone.

    • @Raul28153
      @Raul28153 Před 2 lety +1

      @@indiabiologyexplains5013 Thanks. " model the proteins from the genes," Now I have something else I have to look up and learn about.

    • @indiabiologyexplains5013
      @indiabiologyexplains5013 Před 2 lety +1

      @@Raul28153 Sorry I mistyped that one. I meant that they determine the proteins made by the genes, not "model" them.

    • @Raul28153
      @Raul28153 Před 2 lety +3

      Thanks for the correction. I am trying to teach myself molecular biology esp Crispr CAS 9 gene editing for a project I'm working on. Don't need wet bench skills, just need t be able to write about it intelligently. Things have come a long long way from my days at a program at the Sackler in the 90s

    • @indiabiologyexplains5013
      @indiabiologyexplains5013 Před 2 lety

      @@Raul28153 Nice! CRISPR cas9 editing technology is an amazing field of science that opens an entire realm of opportunity for humans. I hope you learn a lot in your quest to learn more about molecular biology!

  • @TheIamwalruss
    @TheIamwalruss Před 4 lety +2

    Can you explain how this is different or similar from next generation sequencing (NGS) ?

    • @katrinatwing766
      @katrinatwing766 Před 3 lety +8

      The process she is describing with the Illumina NovaSeq is next-generation sequencing. It is 'next-generation' compared to Sanger sequencing. The process she is describing with the Oxford Nanopore Minion is considered long-read sequencing, or has been referred to as third generation sequencing.

    • @jackeldridge1319
      @jackeldridge1319 Před 2 lety +3

      It's similar in the fact that in both cases a DNA strand is grown from Dideoxynucleotropes (ddNTs). These are the As, Ts, Cs and Gs of the DNA strand with two of the hydroxyl groups removed so they don't just bond to one another and make DNA on their own, and need a separate RNA strand to form properly. They just add a dye corresponding to each (e.g red for A, blue for G etc.) and throw it at a string of RNA taken from the DNA you want to sequence and make DNA from it. You then measure the frequency of the dye to figure out which pair is in the DNA and you have your sequence.
      It's different in that in Sanger technology, they grow a single, large piece of DNA using this method which takes ages. In NGS, they use small pieces of very short RNA called ONTs (oligonucleotropes) and they add those to a piece of broken up DNA. They then heat it to split it into two long pieces of RNA through denaturation.
      They then have a chip with ONTs placed on the surface, each on top of a transistor, which the ONTs on the ends of the RNA happily bond to as they're chosen specifically to pair. They then start making DNA of themselves. The chip measures the electrical resistance of each strand as it grows until it has a sample that's just short enough to have grown quickly but just long enough to be a useful sample, and denature it by passing a current through it to heat it up. They then have the perfectly-sized RNA cleave itself through some method or another. Either that's chemical by adding ionic copper to it or mechanical.
      They then grow DNA out of it using ddNTs marked with dyes just like Sanger method, but this time instead of measuring one strand, they measure millions of short ones and compare them between each other and a control sample using a computer. This allows for far faster genomic sequencing, and can be used for more specific applications such as searching for a certain gene. For a complete sequence, Sanger will still be necessary however, but that's frankly quite a specific and highly scientific application.
      You can imgaine how much faster that is going to make searching for something like the BRCA gene or an autoimmune disease in a person's DNA in the coming decades. You could probably have it done at some GPs before long.
      This is my understanding of it, I'm no expert. If there's anybody who knows better what they're talking about: feel free to correct me. This is the best way I could simplify it for myself personally :)

    • @HuFlungDung2
      @HuFlungDung2 Před 2 lety

      @@jackeldridge1319 Could you please clear up something: 'short pieces of DNA' doesn't sound to me like you'd really know how they all fit together end to end, kind of like getting a box full of puzzle pieces without a picture to work from. You could analyze the hell out of each piece, but that wouldn't tell you the picture. Or are they somehow growing entire complete lengths of intact DNA all unravelled?

    • @jackeldridge1319
      @jackeldridge1319 Před 2 lety +1

      @@HuFlungDung2 I think you really misunderstand how DNA works. These methods create RNA, which will always become DNA in the presence of the As, Ts, Cs, and Gs, as they will bond to their subunit mates. They also have a sample made with Sanger that is stored digitally, and they cross-compare it using an algorithm. They line up what traits match up, and what don't in order to sequence the genome. Imagine you cut up a page of a book into tiny strips of paper, but you keep a PDF of the first edition up on your computer. You can get all the info you need from those strips by lining them up and arranging them, as long as you have that PDF up to figure out what adds up, what's different and what's odd

    • @jackeldridge1319
      @jackeldridge1319 Před 2 lety

      @@HuFlungDung2 RNA that's bonded to these subunits won't bond together end to end because they use primer chemicals, such as soaps, that bond to the ends of RNA strands and stop the bonding process, keeping them very short and intact

  • @majorharmonic
    @majorharmonic Před 4 lety

    How much do you think something like this will cost? When do you think they will be available?

    • @SohamMukhopadhyay56
      @SohamMukhopadhyay56 Před 4 lety +1

      You can already buy nanopore devices. Here is the link.
      store.nanoporetech.com/eu/starter-packs

    • @ThiagoNicolielo
      @ThiagoNicolielo Před 3 lety +1

      Prices starting at US$ 1000 only! That's incredible how cheap it already is, and it's portable, low power to operate (USB). And it comes with some consumables even in the start kit. Machines like these probably will change medicine and much more areas.

    • @joramopa
      @joramopa Před 3 lety

      @@ThiagoNicolielo I remember watching a documentary once where it was 10s of thousands of dollars but the presenter said something like, "extremely new technology often sees a exponential reduction in cost as they get more optimized and use fewer and cheaper parts." This was from a while ago. it's $1000 now, another 5-10 years and we'll have consumer level DNA sequencing, or maybe even mass sequencing.

  • @user-ny7zx9tf9f
    @user-ny7zx9tf9f Před rokem

    good

  • @johnsammartino6344
    @johnsammartino6344 Před 8 měsíci

    Batman has been using that pocket sized sequencer for decades. I think someone owes DC comics a royalty.

  • @AdaMs910
    @AdaMs910 Před 3 lety +1

    These all look like photocopiers at first glance

  • @mathUFABC
    @mathUFABC Před 2 lety +1

    i hope in future we will be able to built this sequencing machines out with and Arduino only for hobby. That would be amazing.

  • @interviewkityari6804
    @interviewkityari6804 Před 3 lety

    Nice but incomplete information

  • @707bayshit
    @707bayshit Před 3 lety +1

    I wanna be a student of DNA sequencing 🤓

    • @ge2827
      @ge2827 Před 3 lety

      @GAME SMASHERS hey I need some help in doing my research on ABCB1 in Rough Collie! Please do help me🥲

  • @tashimlo7954
    @tashimlo7954 Před rokem

    how do you sequence something like DNA that is too small to see?

  • @triynizzles
    @triynizzles Před 2 lety +1

    hearing that machine cost as much as an appartment was shocking... i live in CA can you can buy apartments (or condominiums) for less than $700K even in the bay area... that would be quite cheap for a fridge size box that can sequence dna.

  • @Gigatless
    @Gigatless Před 3 lety +1

    no wonder why it's not cheap to read dna

  • @mosbythegr8920
    @mosbythegr8920 Před 3 lety

    The genetic sequencing of a cancer patient.. that should highlight damages, right? And, when knowing what sequence needs repair, that would tell you which amino acids the patient is deficient in. Is "curing" cancer actually as simple as getting the proper combination of amino acids that our body might not be able to produce on its own that also can't be found in regular diets..

    • @ThiagoNicolielo
      @ThiagoNicolielo Před 3 lety +2

      Not being a doctor, I think cancer is not some lack of amino acid, I think it's a mutation that goes wrong. Anyway, I think maybe someday it will be possible for instance, to use CRISPR thechnique, or future substitutes, to fix DNA of tumor cells or something like that.

    • @albertmashy8590
      @albertmashy8590 Před 2 lety +1

      Please leave this to the scientists. Cancer is unregulated cell growth

    • @mosbythegr8920
      @mosbythegr8920 Před 2 lety

      @@albertmashy8590 why would i do that when scientist waste more time than you are apparently aware of. I posed a question.. a good question. If anything, maybe they will learn something. You should try that sometime, instead of thinking everything should be left to half witted sociopathic "scientists"

    • @mosbythegr8920
      @mosbythegr8920 Před 2 lety

      @@bongo3997 Your response had no relevance to what I had originally posed as a question. It was not difficult to understand, though I do see how it is commonly misunderstood. But your response was so painfully.. dumb. It is as if you think you are talking to a child, even though it was you that do not understand. If you do not understand, do not talk. Because, if you do not understand, then what you know, becomes irrelevant

    • @manchuscout4545
      @manchuscout4545 Před 2 lety

      @@mosbythegr8920 Speaking with such authority about the work performance of "half-witted, sociopathic scientists," it's readily apparent @Albert Mashy acquired his formidable expertise after having spent a good many years with his sociopaths, either as a colleague - or as one of their rhesus monkeys. In an act of compassion I'd suggest he offer himself as a test subject for use in treating COVID-19 with Clorox or DRANO, but it's clear he's already done that!

  • @oscarp4328
    @oscarp4328 Před 3 lety

    How much did she say it was ? Lol

  • @OhioBoii330
    @OhioBoii330 Před rokem

    Sooo, even tho she explained it to me, i'mmmmm still not really sure how this works, lmao. But, I'd bet that most people who watched this still have absolutely no idea, lmao. Howeverrrrr, my question is.. whyy don't people get cloned immediately at birth? I mean, wouldn't that save A TON of future health issues?!? Like, say I grow up to be in my mid 20's and end up getting sick which causes kidney failure, or I need a liver or whatever... If I was cloned immediately at birth, wouldn't I very easily be able to use the cloned organs on myself instead of being on a lifelong wait list for an organ?!?

  • @markgearing
    @markgearing Před 2 lety +1

    Title needs to be changed from “how does a DNA sequencing machine work” to “what does a DNA sequencing machine look like”. How DNA is sequenced was glossed over in a couple of sentences, rather like describing how a car works by saying “you add petrol and press the start button”. Disappointing.

  • @mcdk72
    @mcdk72 Před rokem +1

    What a scam