MOREL CULTIVATION: PART II
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- čas přidán 15. 10. 2020
- In this video we go over our second attempt at morel cultivation. Our patch is an eastern facing plot this time with 3 different species of morel mushrooms including a local variety! For more information visit freshfromthefarmfungi.com to purchase our living mycelium cultures and other products visit our ETSY shop FreshFungi www.etsy.com/shop/freshfungi MUSHLOVE
Amazon Mushroom Shop: www.amazon.com/shop/influence... - Věda a technologie
Wonder who thumbs down your video. You do some really neat stuff :) keep it up. Learned a lot from you. I really enjoyed your breeding project with oysters.
Thanks for following along it means a lot! MUSHLOVE
I have a black morel culture I'd love to try to fruit someday, the thought of cultivating morels is a little intimidating. Best wishes for success!
Love the morel experiments. What about simulating the cold by placing you block in the fridge or freezer for a bit?
Thanks for following along that's a cool idea!
Patiently been waiting on these vids lmfao, glad to see it!
Just by listening to you I answered my question about morel liquid culture....mine has color specific to the species fruiting bodies in the mycelium at first I thought contam but those are probably the start of sclerotia
Can you link the research you found information on sclerotia formation? sounds interesting
I probably will try a kombucha scoby mat on some of my areas of morels. The benefical bacteria in those really help regular garden plants to metabolize nutrients. Kombucha is fermented sweet tea, and for each batch, you get a biofilm on the surface of the liquid. It's pretty similar to a "mother" in apple cider vinegar. I think heavy watering is really important too. I think that really helps the mycelium to work down into the soil.
i used fermented liquid kombucha witrh 1/20 to water ratio ,instead of commercial EM1 microbes and yasts worked wonders!
Is it possible for the mycelium to occur up inside the stem of a morel ?
Hmw bro in natural proces in my area in forest grow many morel but bro how can possible to artificial grow morel mashroom ❤❤❤❤
videos rule, thanks. whats with the buds, you listening to house in there?
Hi Garry wonderful work thanks for share with us . What did you use became a substrate and how much ? Thanks again for your knowledge:)
Used sawdust for substrate and oats for spawn (5lb bags)
@@FreshfromtheFarmFungi thanks garry you are the best
Anxious. Any updates on this?
Nevermind just found it
Do you know how to collect Indigenous Microorganisms? (IMO) look into that and try to collect some local bacteria with hydrated rice. I’ve also thought about the cottonwood leaves being a good mulch and also leaves carry microorganisms as well and will create a leaf mold that could potentially be a good source of the symbiotic bacterium you’re looking for.
cool thanks for the suggestions all good ideas!
hi garry again me. can you explain what is TSP ? you are saying sometimes this jar is TSP. and can you give it recipe ? thanks my friend
TSB = tripticase soy broth
@@FreshfromtheFarmFungi you are the one ( the best teacher)
Any PH test ??
no but good idea
If the mycelium is contaminated, could hydrogen peroxide remove the mold?
It’s hard to say for sure I think depends on a lot of factors but would be a cool experiment!
@@FreshfromtheFarmFungi en.psilosophy.info/psilocybe_fanaticus_tek.html#psylocybe_fanaticus_mushroom_museum
He uses antibiotics against mold, of course ...
Excerpt:
"Antibiotic means "anti-life". But interestingly, fungi spores can withstand powerful doses of antibiotics while bacteria succumb easily. That is why "athletes foot" can't be cured with antibiotic creams. It could be that antibiotics come from fungi in particular. When a spore solution is made it can easily be contaminated with bacteria, the most common life form. We are surrounded by them. But with antibiotics, they are very susceptible, especially when in a solution of water and spores. The technique is quite simple. The Antibiotic is put into the spore solution where the bacteria are stopped in a variety of ways, either by cell wall destruction or protein synthesis inhibition, depending on what kind of antibiotic is used. I am no expert in antibiotics, but I know that antibiotics in concentrated form kills them dead, while leaving the fungi spores just fine. And over time, the fungi spores remain viable. And previously, it was bacteria contaminated (no spore germination - just a rotten smell and slime appeared).
But it is important to use the spore solution immediately. Over time, the cultures will still germinate and give a clean culture but you can tell something is not good. The culture is weak and it won't fruit, even though it is clean of bacteria. So use the decontaminated spore solution immediately and your results will work.
Step one: Acquire antibiotics. This is the easiest part. The best place to get a wide variety from Penicillin to Tetracycline is at your neighborhood pet store which has a good fish tank supply and of course, live fish for sale. In a particular section of the store, you can find rack fulls of a variety of antibiotics that are used for fish tank populations. They come in capsule form and are to be used directly into the fish tank water. But for our purposes, this won't work because these antibiotics are not sterile by any means. They are actually heavy with mold. Even Ampicillin you get from your doctor is heavily loaded with mold. The mold spores don't hurt you or the fish but they will destroy your attempt to culture fungi spores.
The antibiotics must be first sterilized before use in spore solutions. Heating the antibiotics is not the way, because it destroys the antibiotics. The antibiotics must be cold sterilized. This is accomplished by a very common micro biological technique ubiquitous throughout the Bio science lab world. The antibiotic is taken out of the capsule, placed into a small jar, about 10cc of water is added, the powder is mixed into solution, the solution is sucked up into a syringe and then finally, the antibiotic water is pushed through what is called a "SYRINGE FILTER". This is a small 25 mm round plastic device that sOcrews on the end of a standard leur lock syringe like the ones the Professor sells. These small round filters come individually packaged in what is called "blister packs" where they are sterile and ready to use. The antibiotic solution comes out of the syringe filter and it is sterile. The filter takes out anything bigger than .2 microns and that includes all spores, bacteria and viruses. The antibiotic molecules get through and are clean of all mold spores plus the bacteria that was in the water is dead already.
The antibiotics that I have used to great success are all available at the pet store fish tank section. Tetracycline is a powerful toxic antibiotic that colors the spore solution orange yellow. Kanamycin is a powerful antibiotic that is clear and does not show up in the spore solution. Neomycin is another clear antibiotic that has worked well. I think Tetracycline might be the best, but Kanamycin and Neomycin work just great. Penicillin is not near as good and usually fails. I have tried Streptomycin which fails half the time and gentimycin which is very weak, fails all the time. Gentimycin is an antibiotic that can withstand autoclaving and is used in agar agar (by such myco supply houses as Fungi Perfecti), but in comparison to Tetracycline, it is weak. In fact, a Doctor friend of mine says they don't use Gentimycin anymore. It's obsolete. I have used chlorpromazine and a few others, and I have found Tetracycline to be about the best, and it is always available at the fish tank store. Plus, it is the only one that colors the water yellow orange (not pretty) and it is the most expensive (less than $10 per pack of a dozen or so pills at the fish tank store)."
@@ytrebiLeurT Using antibiotics to treat mold or any type of fungal infection like athletes foot is completely pointless, antibiotics are for bacterial infections. They kill bacteria. The type of antibiotic used depends on the specific bacteria you're targeting (and any allergy to penicillin's) as what they kill depends on the type of cell wall the bacteria has amongst other factors.
You can get heat resistant antibiotics though, they are used in microbiology for selective cultures like in CRISPR when you want only a specific bacteria to survive in a culture.
Gentamicin is also still used (i gave some to a person like last week), less commonly as the effective dosage has to be calculated on a curve individual to each patient from post infusion blood test and if you fuck up the dosage it can be nephrotoxic.
@@ianb2527 www.fanaticus.com/antibiot.htm
Fanaticus wrote:"...fungi spores can withstand powerful doses of antibiotics while bacteria succumb easily. That is why "athletes foot" can't be cured with antibiotic creams."
"When a spore solution is made it can easily be contaminated with bacteria, the most common life form. We are surrounded by them. But with antibiotics, they are very susceptible, especially when in a solution of water and spores. The technique is quite simple. The Antibiotic is put into the spore solution where the bacteria are stopped in a variety of ways, either by cell wall destruction or protein synthesis inhibition, depending on what kind of antibiotic is used. I am no expert in antibiotics, but I know that antibiotics in concentrated form kills them dead, while leaving the fungi spores just fine. And over time, the fungi spores remain viable. And previously, it was bacteria contaminated (no spore germination - just a rotten smell and slime appeared)."
I trust him...
@@ytrebiLeurT yes but mold is not a bacteria..
Did this end up anywhere, looks like your videos on the bed kinda died off here.
still trialing them it’s a once per
year endeavor
@@FreshfromtheFarmFungi my bad here, I'm now finding out searching your videos doesn't sort them by date. Have you had any fruiting success thus far?
So....no wood ash? Is that just a myth? Everyone, and their dog says to look for morels in forest fire zones....a year or two after a fire. Just wondering if science disproved that, or not?
That is morchella conica (burn morels) this one is morchella esculenta - it grows in grassy riparian areas in cottonwoods and deciduous trees - two different species 👍
@@FreshfromtheFarmFungi Aaaah...thanks, that's nice to know. You just doubled my knowledge of morels....lol.