Thank you. I am hoping to dump every piece of Myco info and experience I have onto the internet :) I hope others use it to take this hobby to the next level!
Thank you so much for posting this!! Finally an actual VISUAL reference to what it is we are supposed to be looking for 👏🏼👏🏼👏🏼 I can’t wait to see the next video with the clamps!!! Bring it onnnnnnnn 🙏🏼🍄💙🍄🙏🏼
Ed you're an awesome guy. Just crazy enough to relate to the normal Myco nerd. By far the best in showing monos and breeding because I haven't seen any other channels showing microscopic mycelium work. Ty sir.
What are the odds! I just watched your video about what monokaryons look like macroscopically yesterday, the one from 5 months ago where you say you won't do a microscope video! 🤣 I also bought a microscope yesterday, amscope, gotta wait 9 days for delivery, can't wait. So this video couldn't be more on point!!! Talk about serendipity! Thanks once again man.
@@edwardgrand Lucky us!!! It’s really super helpful to see things in context, and get an idea what it is I’ll be looking for. It’s going to take me a while I’m sure, but really excited to head down that rabbit hole. Partly thanks to you man. 🙏
This really shows me how much harder it can be looking at something for a lack of without compared to with because you got to cover more area and more identification is involved.. and how important it is to learn because confirming by saying tomentose could mean monokaryotic and rhizomorphic could be dikaryotic can only go so far.
Nice walk through the plate. I certainly don’t have the experience to do this yet. I’ll know where to shop for monos and how to check my results. Look forward to the next one. ✌️
I think I might need some Panadol before I start doing this 😂 I'm nowhere near ready but I will be at some stage. I'm fully addicted. It's better than my previous addictions though by faaaaaar!!
One of the main reasons for making these videos was to prompt people to share/sell/innovate. People have been sitting on their coveted monos for years. Time to share.
Thank you Ed. You gave me inspiration for change. I stopped smoking weed. Don’t plan on doing it again. I have 6 confirmed monos now. the possibilities add up quick. today I pulled 5 putative monos from zaps. I’m going to try P. zap x P. subtrop. Although idk if it would be a significant difference since they are so close to begin with. Also idk if it would actually have any advantages over just doing one or the other. Idk just thought it sounded cool so I’m going with it. QQQ do you think I would need to use protoplast fusion to accomplish this?
I am not familiar with the taxonomic/genetic differences in P. zap and P. subtrop. They will need to be very closely related to breed naturally. Good luck. I am not really into protoplasts or artificial genetic manipulation.
This is great Ed! But I’m not that far in mycology to be breeding! I don’t even have a microscope yet but I know you take your transfer in like 3days but I’m not scoping anything I just want to transfer to another agar plate to keep growing so I can add it to grain!
Hi Dr Ed, I was wondering if you know where to get tween or is there anything else that would do what it does? It keeps the spores from clumping correct?
Online. It is referred to as 'polysorbate 20-80'. They use it in cosmetics. If you can't find it, use a very, very, very tiny drop of liquid dish soap.
Dude... it'd be so sick to sic CRIPSER on these, just one level down from what we see here... As infinite as that is, one more notch would be a neat exploration.
Awesome. I’m a frequent denison of the Kingdom. Krung Thep and beyond. What part of Thailand are you in? Do you do much field work there? Best seasons for photography and spore collection. Not just active mushrooms. Edibles. I’ve seen them in April - July around freinds place near Rayong. Also Hua Hin. How difficult is it to get Flow hood? Needles in Thailand? Great job!! Krup Khun.
@@edwardgrand Very cool. Every day I wonder why I’m in US at all. I was watching one of your videos and heard you remark about the price of the Thai equivalent to the ink Bird controller. Being $10 instead of $100. Same with everything. Had Pad Thai a week ago $22 USD. And not good. Sabai Sabai.
Question: I made agar the other day and they seemed clean but then I did a transfer the next day I stared seeing specks on the agar around the edge then today I can actually see Bacteria growing on the specks with my eyes! Not microscope! It’s not touching the transfer do you think I can move it to another plate without getting contaminated?
@@edwardgrand damn it’s been almost a month and I do see some germination like it’s crawling out of the crack from the gnd! But it’s been about 2weeks on I can’t see anything yet on the Alabama f2s maybe with this new agar recipe it will grow faster!
I started trying bag tek and my apes are starting to turn blue and they are only half or not even halfway done what could be causing this not enough FAE?
@@edwardgrand I thought I might try it and my pin set is awesome way better than the tub I had! They were just a few here and there but I did get a little bit over an oz in 3 flushes! But I think this one in the bag is going to be way better! I think I’m going to do a big 64qt monotub in the next few weeks! Dubtub next probably! That’s what the tub was I did last!(dubtub)
hey ed, you should look into getting or making a "darkfield microscope filter". it's just a simple disc that blocks out the light in the center and practically inverting the colors in your view. (black background, white specimen / mycelium. it's waaaay better to look at. thanks for your videos, keep it up brother
Ed you are the most generous mycologist on the internet. Thank you for passing your knowledge on so freely. You have changed the game forever.
Thank you. I am hoping to dump every piece of Myco info and experience I have onto the internet :) I hope others use it to take this hobby to the next level!
@edwardgrand we'll bring it to the next level because of you❤
I know you've been reluctant to do a video like this and it's a pain for you to setup, but I found it really interesting and appreciate it. Thank you!
Excellent. Thanks for showing some microscopy and talking through it.
Thank you so much for posting this!! Finally an actual VISUAL reference to what it is we are supposed to be looking for 👏🏼👏🏼👏🏼
I can’t wait to see the next video with the clamps!!!
Bring it onnnnnnnn
🙏🏼🍄💙🍄🙏🏼
Ed you're an awesome guy. Just crazy enough to relate to the normal Myco nerd.
By far the best in showing monos and breeding because I haven't seen any other channels showing microscopic mycelium work. Ty sir.
Thanks man...this was perfect. Mush appreciation
Doing the Lord’s work 🫡
Super helpful!
What are the odds! I just watched your video about what monokaryons look like macroscopically yesterday, the one from 5 months ago where you say you won't do a microscope video! 🤣
I also bought a microscope yesterday, amscope, gotta wait 9 days for delivery, can't wait.
So this video couldn't be more on point!!! Talk about serendipity! Thanks once again man.
I finally got a good microscope mount. The other one was shit.
@@edwardgrand Lucky us!!!
It’s really super helpful to see things in context, and get an idea what it is I’ll be looking for. It’s going to take me a while I’m sure, but really excited to head down that rabbit hole. Partly thanks to you man. 🙏
This really shows me how much harder it can be looking at something for a lack of without compared to with because you got to cover more area and more identification is involved.. and how important it is to learn because confirming by saying tomentose could mean monokaryotic and rhizomorphic could be dikaryotic can only go so far.
Thank you! This came at a good time for me. Looking forward to the next one.
Ed, you rock. Thank you for your time and epic catch phrase. "caffeine and gas mycology" needs to be your company.
Nice walk through the plate. I certainly don’t have the experience to do this yet. I’ll know where to shop for monos and how to check my results. Look forward to the next one.
✌️
It's like where's Waldo except Waldo is a mono tone trace of fine microscopic lines. Lol.
They said it couldn't be done. Thanks for doin' it!
🔬Excellent camera work! Thank you for the tutorial, Ed. 🙏🏻
Your videos pushed me over the brink of getting a microscope. Thank you good sir.
You won't regret it. One thing that will literally last a lifetime!
🎉
I think I might need some Panadol before I start doing this 😂 I'm nowhere near ready but I will be at some stage. I'm fully addicted. It's better than my previous addictions though by faaaaaar!!
Hello from KCMO Dr. Ed 🤙
Very informative thanks
Thanks for the info
🤙🏻🤙🏻...from Virginia. Love watching and absorbing you're stuff man. 🍄💙
-mushmellow man
Awesome topic and show...I saw someone selling mono's now here in USA, not yours but its going down 😮
One of the main reasons for making these videos was to prompt people to share/sell/innovate. People have been sitting on their coveted monos for years. Time to share.
Love your internal dialog Makes it flow so well, thanks brother....😊
Thank you Ed. You gave me inspiration for change. I stopped smoking weed. Don’t plan on doing it again. I have 6 confirmed monos now. the possibilities add up quick. today I pulled 5 putative monos from zaps. I’m going to try P. zap x P. subtrop. Although idk if it would be a significant difference since they are so close to begin with. Also idk if it would actually have any advantages over just doing one or the other. Idk just thought it sounded cool so I’m going with it. QQQ do you think I would need to use protoplast fusion to accomplish this?
I am not familiar with the taxonomic/genetic differences in P. zap and P. subtrop. They will need to be very closely related to breed naturally. Good luck. I am not really into protoplasts or artificial genetic manipulation.
This is great Ed! But I’m not that far in mycology to be breeding! I don’t even have a microscope yet but I know you take your transfer in like 3days but I’m not scoping anything I just want to transfer to another agar plate to keep growing so I can add it to grain!
Are you going to do the rest of the video?😂
Maybe in a few days.
Hi Dr Ed, I was wondering if you know where to get tween or is there anything else that would do what it does? It keeps the spores from clumping correct?
Online. It is referred to as 'polysorbate 20-80'. They use it in cosmetics. If you can't find it, use a very, very, very tiny drop of liquid dish soap.
Tyvm@@edwardgrand
Dude... it'd be so sick to sic CRIPSER on these, just one level down from what we see here...
As infinite as that is, one more notch would be a neat exploration.
I think we should stay away from genetically manipulated mushrooms. They are perfect as is.
❤❤❤
Awesome. I’m a frequent denison of the Kingdom. Krung Thep and beyond. What part of Thailand are you in? Do you do much field work there? Best seasons for photography and spore collection. Not just active mushrooms. Edibles. I’ve seen them in April - July around freinds place near Rayong. Also Hua Hin. How difficult is it to get Flow hood? Needles in Thailand?
Great job!! Krup Khun.
I am outside of BKK. Used to live in CM. FFU are readily available online like everything else now. Just have to be patient.
@@edwardgrand Very cool. Every day I wonder why I’m in US at all. I was watching one of your videos and heard you remark about the price of the Thai equivalent to the ink Bird controller. Being $10 instead of $100. Same with everything. Had Pad Thai a week ago $22 USD. And not good. Sabai Sabai.
It's quite easy to get out. Flights leave every day ;)@@TheCragg777
Question: I made agar the other day and they seemed clean but then I did a transfer the next day I stared seeing specks on the agar around the edge then today I can actually see Bacteria growing on the specks with my eyes! Not microscope! It’s not touching the transfer do you think I can move it to another plate without getting contaminated?
Yes. Move it to a new plate.
@@edwardgrand sweet thanks man
You said you do transfers from spore as soon as possible like 3 days! Can you see germination that soon with your naked eye?
yes
@@edwardgrand damn it’s been almost a month and I do see some germination like it’s crawling out of the crack from the gnd! But it’s been about 2weeks on I can’t see anything yet on the Alabama f2s maybe with this new agar recipe it will grow faster!
what magnification do you use?
400X total magnification for clamps (10X eyepiece, 40X objective)
1000X for spores
I started trying bag tek and my apes are starting to turn blue and they are only half or not even halfway done what could be causing this not enough FAE?
Bags are still a bit of a mystery for me.
@@edwardgrand I thought I might try it and my pin set is awesome way better than the tub I had! They were just a few here and there but I did get a little bit over an oz in 3 flushes! But I think this one in the bag is going to be way better! I think I’m going to do a big 64qt monotub in the next few weeks! Dubtub next probably! That’s what the tub was I did last!(dubtub)
Can anyone recommend a scope?
Amscope b120c
@@edwardgrand thanks Ed
hey ed, you should look into getting or making a "darkfield microscope filter". it's just a simple disc that blocks out the light in the center and practically inverting the colors in your view. (black background, white specimen / mycelium. it's waaaay better to look at. thanks for your videos, keep it up brother
Thanks for the suggestion. I used to use one of those, but I prefer this way. Thanks for watching.
I can't get darkfield to show anything over 400X on a smash mount
No need to use darkfield.@@55Reboot
Thank you Ed