Haemoglobin Test Procedure | Hgb Blood Test Method | Blood Hemoglobin

Hb Blood Test Method / hgb blood test: Complete hemoglobin estimation procedure with results. we have used Sahil's method to estimate blood Haemoglobin level i.e. Normal Hb level. Haemoglobin Count in Blood is also called Hb count or #haemoglobintest.
For detailed information visit: www.ebiologyla...
Principle of Haemoglobin Test Procedure:
Blood is mixed with N/10 HCl resulting in the conversion of Haemoglobin to acid hematin which is brown in color. The solution is diluted till its color matches with the brown-colored glass of the comparator box. The concentration of Haemoglobin is read directly.
Haemoglobin Estimation with Sahli’s/acid hematin Method:
Equipment required for Hb Count:
Hemocytometer: amzn.to/3cQIx9q which consists of a comparator box that has brown colored glass on either side Hb pipette which is marked up to 20mm3(0.02ml blood) Tube with markings of Hb on one side
glass rod dropper.
Sterile Cotton: amzn.to/3gC0rhm
Reagent Bottles: amzn.to/3xvRBZj
Reagents required for Hb test:
N/10 HCl
Distilled water
Sample: Venous blood collected in EDTA as described earlier
The procedure of Hb Count:
1. Add N/10 HCl into the tube up to mark 2g%
2. Mix the EDTA sample by gentle inversion and fill the pipette with 0.02ml blood. Wipe the external surface of the pipette to remove any excess blood.
3. Add the blood into the tube containing HCl. Wash out the contents of the pipette by drawing in and blowing out the acid two to three times. Mix the blood with the acid thoroughly.
4. Allow to stand undisturbed for 10min.
5. Place the haemoglobinometer tube in the comparator and add distilled water to the solution drop by drop stirring with the glass rod till its color matches that of the comparator glass. While matching the color, the glass rod must be removed from the solution and held vertically in the tube.
6. Remove the stirrer and take the reading directly by noting the height of the diluted acid hematin and expressing in g%.
Advantages: blood hemoglobin method
Easy to perform
Quick
Inexpensive
Can be used as a bedside procedure
Does not require technical expertise
Disadvantages: Hemoglobin Test Procedure
Less accurate.
All haemoglobins (oxyhemoglobin, sulphemoglobin) are not converted to acid hematin and hence the value of Hb obtained is less than the actual value.
The colour of the acid hematin develops slowly.
Colour of acid hematin fades with time and dilution must be done exactly after 10 min when the colour development is maximum
Individual variation in the matching of colour is seen.
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[@DKBiosolutions]

Hello guys 👋, in this video I forgot to mention that while performing the experiment, do not take out glass rod from the tube. It may change your results.
Best luck for your Experiment 😊👍💐

[@samreenkhan8454]

Tq sir

[@ismailmalik3648]

I have gone through around 30 videos regarding histopathology but your video was best among all.. The prick you gave to ur self is commitment towards dedication.

[@DKBiosolutions]

Thank you so much...

[@randomstuffswithrrd]

first class.. best thing is you show the whole practical live.

[@DKBiosolutions]

Thank you for your appreciation 😊👍...

[@shashankrao6040]

Awsum video . This really helped me for my exams ....Tysm..😊

[@DKBiosolutions]

I'm glad you found it useful... 😊👍

[@izzahadnan9176]

Explained in the best possible way

[@DKBiosolutions]

Thank you 😊

[@karthiklal7763]

It is very helpful to do my practical exam, Thank you Very much,😎😎😃..........

[@DKBiosolutions]

Hello Karthik...
Best luck for your Practicals... 😊👍

[@karthiklal7763]

☺️Thanks☺️

[@qataarqato1367]

Thank you from Somalia 🇸🇴

[@DKBiosolutions]

My pleasure... I am glad to know that it helped you 😊

[@chetanchaudhari2442]

Literally help alot my sir take me out during this practical this video help me 😅😅❤

[@DKBiosolutions]

My pleasure 😊

[@SkBhagora-gh8bd]

Thankyou ☺️😀😀

[@DKBiosolutions]

Welcome 😊

[@Shaheeda345]

Thanks bro
Nice presntation

[@charliebrushwood2053]

Very good and funny videos bring a great sense of entertainment!

[@DKBiosolutions]

Thank you for your appreciation 😊👍

[@muhammadnoman3576]

Thank you for your Information

[@shootingstar9163]

It was best video 😮

[@DKBiosolutions]

Thank you so much 😊🙏

[@alejandracabrera2160]

thank you. Very informative

[@kesharsharma5617]

Very helpful video for me

[@DKBiosolutions]

Glad to hear that 😊👍...

[@Mohmmadmusa-r3y]

That unhappy face after knowing 12 hb 😂😂

[@DKBiosolutions]

😁😁😁

[@christinaeda]

Wow....!! thank you so much for this video it's very helpful 🤩

[@DKBiosolutions]

Glad to hear that it helped you ☺️👍...

[@narimansaad4038]

Thanks that was really helpful

[@DKBiosolutions]

Happy to hear that it helped you 😊👍...

[@afreenkhalil1346]

Thnkss its helpful 👍🏻

[@DKBiosolutions]

My pleasure 😊👍...

[@shabanakhilji5813]

Thanks for the efforts 👏🏻👏🏻👏🏻👏🏻

[@DKBiosolutions]

My pleasure 😊👍

[@atulkumarrao1786]

Very nice very good.thanks

[@DKBiosolutions]

😊👍

[@naskatram7248]

Thank u

[@DKBiosolutions]

My pleasure 😊👍

[@devindersingh4104]

Do we have to suck the blood through rubber tube or do we have to let is get sucked up by capillary action?
When I pricked my finger to let the blood into the haemometer, it never went into it. Please tell what is the problem here. What I could have done wrong?

[@DKBiosolutions]

Yes... You have to suck it... Capillary action may not work every time... So many factors affects capillary action... Blood composition, environmental conditions and so...

[@lals573classes]

Gud information

[@DKBiosolutions]

Thank you 😊

[@samratskvlog5292]

Thank you for this video ❤️

[@DKBiosolutions]

My pleasure 😊

[@samreenkhan8454]

Superb
Really very helpful

[@DKBiosolutions]

Thank you Samreen😊👍...

[@simrandeepsingh13]

1:10 , its 0.1 N HCL not 1 N HCL

[@afshaansari3822]

Good

[@DKBiosolutions]

Thank you 😊

[@moistorphan]

this was helpful thank you!

[@DKBiosolutions]

Glad to hear that 😊👍..

[@frankycool1442]

Thanks

[@michellelarissa7154]

Thanks a lot. This video is very understanding. Please can I have some referenced articles on this?

[@DKBiosolutions]

My pleasure... I will search for articles and will provide you links... Tomorrow evening...

[@michellelarissa7154]

@@DKBiosolutions OK thanks. Will be waiting. Its will be very helpful for my Research. Thanks

[@michellelarissa7154]

Hey

[@DKBiosolutions]

bmcpublichealth.biomedcentral.com/articles/10.1186/s12889-020-08537-4

[@user-sz2bw3ps2c]

Thank you ❤️

[@DKBiosolutions]

My pleasure 😊👍

[@vaishubhangre2804]

Nice 👍👌

[@harshita20107]

How many tests can we make with this kit and after we wash the glass rod can we use it again

[@DKBiosolutions]

Any number of tests can be done till you have blood samples... Yes you can use glass rod after washing for next test...

[@ruturajswain8409]

Today i test my haemoglobin , the percentage was 19.5 it's not normal waht cal i do now?

[@DKBiosolutions]

Visit your doctor...

[@ruturajswain8409]

@@DKBiosolutions then again test it shows 17.9 . I test in machine it shows 17.9 but in normal test it shows 15.4 which one is correct

[@DKBiosolutions]

Both are normal readings... If you feel anything irregular... Visit your doctor...

[@ruturajswain8409]

@@DKBiosolutions ok brother

[@muhammadjunaidali769]

awesome

[@DKBiosolutions]

Thank you

[@shilpa2788]

Super 👌

[@DKBiosolutions]

Thank you 😊👍

[@RajaDurai-px1yu]

Super i am technology good hB

[@abinaya5949]

Distilled water mean?

[@DKBiosolutions]

Pure water... No impurities

[@abinaya5949]

@@DKBiosolutions ok thanks

[@viralbgmcollection]

Super bro♥️ love from kerala

[@DKBiosolutions]

Thank you 😊

[@rrajagoswami881]

Sir hb meter me 12 or 14 ke beech jo line rahta hai us line ko ek line ko kitne number me count karenge

[@DKBiosolutions]

13 ka line hai o ..

[@RITESHKUMAR-ok4jo]

Nic vio .

[@hasoni411]

منورين طلاب صيدله كركوك

[@lavinmuhammed3834]

About the result
My teacher said near the end point take three readings : when the color of the mixture is slightly darker, when the color is exactly similar and when the color is just slightly fainter and I didn't understand why we take those readings :(
But I didn't see those steps in your vedio
Can you just explain what my teacher mean by those readings? Reply to my comment plesss

[@DKBiosolutions]

Hello...
I have never tried these steps. But your teacher may be want you to take mean of these 3 readings for final calculation.

[@lavinmuhammed3834]

@@DKBiosolutions so finel calculation is deferent frome hb measurement?
And what is finel calculation used for?

[@DKBiosolutions]

No no... It may help you to get exact hb count... Reading will be same at the end...

[@lavinmuhammed3834]

@@DKBiosolutions ahaa i got it,,,, thank youuuuu soo much dear ✨✨✨
and really your vedio is so usfull thank youuuuu agaiinn 😊💕

[@DKBiosolutions]

My pleasure 😊... Thank you so much for your kind words 😊👍...

[@athiraathira7580]

Sir last How to see measure the tube

[@DKBiosolutions]

There are markings on tube. You just need to see and take a note of it.

[@athiraathira7580]

@@DKBiosolutions thanks for your reply iam understand

[@microcarediagnostics2941]

HB 11.4

[@shubhamkumarverma6]

Why 0.1N HCL in brown bottle??

[@DKBiosolutions]

No specific reason... That bottle came with the kit..

[@shrikantmali8321]

Super

[@r.yogith2396]

Why HCL is added in this test

[@DKBiosolutions]

When blood is mixed with 0.1 N HCl (N/10 HCl), the Hb is degraded to give rise brown-colored acid-hematin. The intensity of color of acid-hematin is directly proportional to the concentration of Hb.

[@acquire19]

Blood lene wala tube har baar naya hona chahiye kya...?

[@DKBiosolutions]

Na...

[@yellowindian5134]

Why is ur haemoglobin low?

[@DKBiosolutions]

It may be because the chemicals I have used are low quality... Or it May be because my hb was low in real...

[@aalaporantamilan3317]

Blood is how will be taken into pipete.

[@DKBiosolutions]

You have to use mouth to draw that blood drop into pipette...

[@thebiomarhealth612]

Great job

[@DKBiosolutions]

Thank you 😊👍...

[@croisane]

which numbers do we interpret as our hb?

[@DKBiosolutions]

Yellow marked numbers, g%...

[@ashoknayaki7776]

You take slide blood

[@rushabhjain2115]

Sir why 0.1N HCL is added

[@DKBiosolutions]

Brown colour of the comparator glass is equivalent to the colour of acid haematin produced using N/10 Hcl for blood sample of 14.8 gm%. Test solution is compared against it. Therefore no other strength of HCL Should be used

[@DKBiosolutions]

Hemoglobin is converted by HCl into acid haematin which gives brown colur to solution
This colour of the solution is matched with the colour of the comparator of sahlis haemoglobinometer

[@rushabhjain2115]

@@DKBiosolutions Thankyou

[@dharanidharani5528]

Method name

[@DKBiosolutions]

Haemoglobin Estimation with Sahli’s/acid hematin Method

[@thesunilbutiya]

osm

[@fei_a_z1193]

Background music is irritating

[@DKBiosolutions]

I'll improve next time 🙂👍

[@mrsah_01]

Count HB% 10.2 yaa 10.3

[@akshatsaran7497]

Sir es kit ka price hai

[@DKBiosolutions]

Hi... My apology for late reply. You can buy it from online stores too... Price will be around 1k rupees.

[@shaktisunery2833]

क्या पूरे 8minut रखना जरूरी है

[@DKBiosolutions]

In those 8-10 minutes RBCs are lysed and Hb gets converted in to acid haematin completely... so yes.... 8-10 minute रखना जरूरी है...

[@atheeribrahim2636]

ليه مسمينه سهلي وهو صعبي! [.

[@DKBiosolutions]

Hello Atheer...
I would like to help you if you have any queries. Please translate your comment in English. Thank you 😊👍

[@atheeribrahim2636]

DK Biosolutions
Thank you very much if I have any problem I’ll send you 🥺💛

[@DKBiosolutions]

You are welcome 😊👍

[@narimansaad4038]

هههههههههههههههههههههههه

[@easyartshorts4993]

Do we have to suck from mouth or is it capillary suction?

[@DKBiosolutions]

Use mouth to suck blood into pipette.... Carefully 😊👍

[@easyartshorts4993]

@@DKBiosolutions okay 👍

[@jaisumisumi1615]

👌👏tq sir

[@sabavathnaresh8458]

Helpful avuthunthi ani anukuntunanu

[@AfsanaParween-pk9hz]

❤❤❤❤❤

[@kesharsharma5617]

Last moment is very funny.. Your mouth is ☹️🤣🤣👍

[@DKBiosolutions]

☹️☹️☹️👍

[@ashoknayaki7776]

Pipte infection attha

[@vidiablue]

My finger felt the needle😖

[@tranzindiarethika5145]

Iam lab technician lab work please

[@lajoswinkler]

Never use 90 % ethanol. It is less germicidal than 70 %. You should know that.

[@DKBiosolutions]

Thank you for your suggestion... 🙏

[@kavitaanjali3808]

Tq u (. ❛ ᴗ ❛.)

[@DKBiosolutions]

My pleasure 😊👍

[@TkarmugilanECE]

786

[@juharAmeumare]

Demon menstruation

[@DECODINgTeach]

U r woman no worries dont be unhappy by 12😅

[@ashoknayaki7776]

Wrong procedure

[@DKBiosolutions]

Please develop your own procedure... This procedure is not mine... But it's there from long time...

[@ashoknayaki7776]

Insert blood drop slide