Expi293: Transient HEK 293 Expression Protocol
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- čas přidán 7. 12. 2015
- Learn how to produce recombinant proteins with the Gibco™ Expi293™ Expression System and achieve gram per liter protein yields in a transient HEK 293 system. www.thermofisher.com/us/en/ho...
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Are these vented cap flasks? cat # ?
So Does Pfizer VAccine uses this one, HEK-292 or 293 correct for VAccines 2020...(+) covid-19? anyone, comments,
no they use an IVT reaction and no cell production
Yes they use it. In the vaccines and or testing.
@@heatherwhitehead3743 ? I don't think they use cell production for either of those ?
Is it needed to lyse the cells with lysis buffer? My protein is a cytosolic protein. Thanks!
Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion and of our agents can assist you with your questions.
Hi, adding enhancers 24 hours after transfection also works? Or does it have to be within 18 hours?
24-hours is fine
from human to beast... lucifer is happy..wtf
hail satan
I know science can seem spooky sometimes, but can you pls explain to me why you think expressing proteins using cells is satanic? I'm baffled lmao
making us all cannibals...
I believe it was Eleanor Roosevelt who once said "no one can make you a cannibal without your consent".
People need to search more about those things, before posting this kind of comments... Years of college and studying to read a comment like this...
@@thermofisher you are a terrible person
@@jackandwz I laugh at your years of indoctrination.
Jesus protects fr he brings up college like only he knows the answers 😂 he’s a shiller along with this disgusting account
Add diluted ExpiFectamin TO diluted DNA??? The protocol says "Add diluted DNA to diluted Expifectamine."
We apologize for the delay in response. Typically, we add diluted transfection reagent to diluted DNA, but both methods will work and give comparable results. Thanks for your question.
How much did you sell your soul for? Babies are not for consumption by God fearing people.
@@thermofisher answer the other comments, people are angry and have answers. But you only make things worse by saying stuff like "you cant become a caníbal without your consent" to someone who called this cannibalism. You, indeed, are a terrible terrible person.
@@JesusProtects what I think he means is be informed know what your eating...when one goes to a fast food chain or anywhere out to eat one really never knows what they are eating..fried green tomatoes and soylet green come to mind...
😃
Human embryo kidney.
293 tries to get it.
Maybe explain what monsters you are while at it!
Yes from aborted female babies! Murder!
You know they don't harvest these from human embryos right? 😂