Im not sure if Im thinking about this right, but basically, since we can differentiate between fragment sizes based on the electrophoresis (assuming the gel can differentiate sizes of fragments by just 1 base pair), we can determine a sequence simply by analyzing where each fragment migrated in each tube based on the ddNTP? If that is correct, then how do we confirm the starting base pair of the sequence? I get that it would be the smallest fragment on the gel, therefore the band that traveled furthest, but that band can be a fragment that has 1 dNTP before the ddNTP, so that wouldn't tell us the very 1st base pair of the sequence necessarily. So in other words, how do we know that the smallest fragment had a ddNTP at the first base pair after the primer? Im not sure if that question makes since. But great video anyway, really clear.
How to calculate the number of fragments with different lengths? From short to long are N (Number of DNA template) x p (Termination probability), N x (1-p)*p ... ? Is it correct? why the band in the real gel is not like this?
3:13 "when I added all those things in it" 😭😭 what things ?? please avoid saying vague THINGS like that . throughout the video, there were a couple vague words you used like that. Otherwise, the topic would have been clear .
It is safe to say that this is the clearest video about Sanger Method I've found on CZcams by far, thanks for your work!
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I couldn't agree more.
Exactly same words by me
100%
Lady, you just rescued my Biochemistry grade. Much appreciation.
Thank you 😊
God bless you I watched 3 lectures but could not understand the last part you made it clear
Thank you❤
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To the point video ever found
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Omg, i love u, my apresentarion of Master's degree is tomorrow and I was confused on this topic. You save me
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beautiful video, I'm currently doing my masters in Clinical immunology and was stuck on Sanger DNA method. THANK YOU
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Best explained
Thank you 😊
Im not sure if Im thinking about this right, but basically, since we can differentiate between fragment sizes based on the electrophoresis (assuming the gel can differentiate sizes of fragments by just 1 base pair), we can determine a sequence simply by analyzing where each fragment migrated in each tube based on the ddNTP?
If that is correct, then how do we confirm the starting base pair of the sequence? I get that it would be the smallest fragment on the gel, therefore the band that traveled furthest, but that band can be a fragment that has 1 dNTP before the ddNTP, so that wouldn't tell us the very 1st base pair of the sequence necessarily.
So in other words, how do we know that the smallest fragment had a ddNTP at the first base pair after the primer?
Im not sure if that question makes since. But great video anyway, really clear.
Needed this last year): but thank u for finally understanding this😍😍😍
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Thank you
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Thank you for the informative videos
You are welcome dear
How to calculate the number of fragments with different lengths? From short to long are N (Number of DNA template) x p (Termination probability), N x (1-p)*p ... ? Is it correct? why the band in the real gel is not like this?
Thank you thank you thank uuuuu
You are welcome 😊
My Goly, I get it!
That's great thank you 😊
Very nice
Thank you
Finally I get it haha
3:13 "when I added all those things in it" 😭😭 what things ?? please avoid saying vague THINGS like that . throughout the video, there were a couple vague words you used like that. Otherwise, the topic would have been clear .
Thanks!!!! 🤍🤍🤍🤍🤍💗
You are welcome 😊