RNA extraction using trizol method
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Congrats for the excellent explaining of that method
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Thank you, that is amazingly clear
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what does it mean if the pallet turned yellow after adding isopropanol?
In rna extraction with trizol why the upper phase is red, phenol-chloroform phase and the lower phase is aqueous ?
Usually the clear aqueous phase is on top after centrifuging, but under certain conditions the aqueous phase can end up denser than the organic. That's why they include a red color for the organic phase in trizol, can always find the aqueous layer.
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Where does dna go?
In the past 5-10 years the "molecular biology world" changed their path of research due to the RNA importance and variability in the field of experiments. So don't be so confused. Keep in mind that most of the viruses and RNA!
Interphase
Which type of rna is isolated
Total rna is extracted
You can seperate the RNA based on basepair length by adding different concentrations of alcohol. When first you add just a "little" alcohol only the smallRNA will flowthrough and rRNA will stay in the collumn. Then when using a higher alcohol percentage, the rest of the RNA can be extracted.
But you didn't degrade DNA.
Trizol allows for separation of DNA and RNA. For acidic phenol solutions (like trizol) RNA ends up in the top aqueous phase (almost always top phase), and DNA separates to the lower organic phase (partially the interphase).