Hi Ma'am, thank you very much for such a good,short & crisp but very much informative video on agarose gel electrophoresis.... I've a question on the differential mobility profile of nicked & linear DNA molecules of same size; why do linear DNA molecule migrates ahead of nicked one? I'll will be grateful to hear from you. Thank you!
so this isn't used to be able to look at the dna letters? Thanks I've had trouble trying to find the videos where you take the dna and look at the actual proteins. maybe i will find it soon :)
Not sure if the comments are still answered, but... Why do we need a PLATINUM wire electrode in the electrophoresis setup? What exactly will happen if one replaces that electrode with an ordinary Copper wire?
Please upload some videos about difference between RNA and DNA isolation and separation techniques. Do tell about Difference in their agarose gel concentration and gel separating chamber, where we use horizontal or vertical gel chambers...
Hi this video is great! I was wondering if the thickness of the band would suggest any properties about the nucleic acid strand, i.e there are more nucleic acid fragments of that size?
Great video, thanks! I have a question concerning visualisation: If after the gel we have the blue bands with the loadind dye, why do we need to add Ethidium bromide to make it visible under UV? Isn't the information we have from the blue bands sufficient? Or is it maybe, that the blue bands we see after we ran the gel is maybe almost only the dye itself, as it leaves the DNA (or RNA) and just sinks faster to the + side, so that our actual DNA (or RNA) is located a bit above the dye - and that is why we need to check it under UV, as it would be "invisibly" located above the dye? Really can't find any answer to this! Thanks again! (:
We either add GelRed right into the gel or we use ethidium bromide later for visualization; both are carcinogens but we would not use both at the same time.
Are PCR and then AGE being done in an automated fashion on a single sample in a miniature electrochemical device, such as one that can only be used once ?
Hi! I just found you and I have been watching your videos. They are amazing! I was wondering (this may be a silly question) but how do you know which restriction enzymes to use for example for paternity testing? does it matter which to use as long as all DNA samples are treated the same? thank you! look forward to watching all your videos--Claudia
Hei .. thank you for your comment ... as you said, you should treat all the samples with the same restriction enzyme .. and experimentation will show us which is the best restriction enzyme to use :)
I have a question .. when migrating the genomic DNA .. shouldn't multiple bandits appear, each expressing a single chromosome content .. since chromosomes carry DNA of very different sizes, especially if we are talking about humans, for example
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
A complete and very easy to understand explanation (like the videos for Flow Cytometry and FACS) on the principles behind the technique.
She is great a great teacher! Respects from Sindh!
A great teacher..... Thanks a lot... Tomorrow is my exam... Very useful
Love from Cameroon 🇨🇲 thank you
بجد احسن حد شرح الموضوع دة بالتوفيق❤
This video is very helpful for me. Thank you u explained in easy way 🙂
thank you so much maam, for your wonderful explanation of Principle of Agarose gel electrophoresis.
You are the best, no one like you
Thank you so muck mam.Because of u i can able to understand the concept now mam.
Will see if you already did capillary electrophoresis, this was such a fine explanation thanks.
Very very well explained...very helpful....people out there can try watching this vid
Thank you! we have never add the colour right into the gel tho.. but we do add it to the samples we are running on the gel.
One of the best youtube chanel l ever seen. :)
Late to the upload but I’ve watch almost all your video and they all been so helpful:) ofc ill like and subscribe!
your explanation is second to none.
You are the best molec teacher, who I have ever seen!!! Well done 👍😊 and thanks for the Videos. What is your name by the way???
mem i like your explanation, i love to hear u r voice again and again u r a great talent .. and i really love..
why do indian people always says mem or ma'am
@@briang1310 coz it is taught and its logical as well to respect the efforts
@@SandeepKumar-dd3ie learn respect then
Very well explained.... thanx alot for making such video. This helped a lot in my thesis research. Please continue to teach us :))
Thank you for your support :)
Wow, this video is so clear and useful, besides you are awesome, thank you so much
Well explained! Good explanations linked with real life applications.
Your voice like best for presenting and I have get many from your channel
Hi Ma'am, thank you very much for such a good,short & crisp but very much informative video on agarose gel electrophoresis.... I've a question on the differential mobility profile of nicked & linear DNA molecules of same size; why do linear DNA molecule migrates ahead of nicked one? I'll will be grateful to hear from you. Thank you!
Fantastic, I learned a lot and I didn't know anything about the subject.
😊😊😊😊Love it here plz make more videos on biology concepts especially 4 varsity students
Concise & informative! Good job. 🙏
Concept well explained. Thank you
Very well explained and easy to understand. Thank you
very informative video, explained in detail for the reason of each step.
You've nicely explained the topic. Many thanks for sharing ♥️
great video!!really helped me in my test..thank you so much
Very well explained, you are an awesome teacher. I SUBSCRIBED
so this isn't used to be able to look at the dna letters? Thanks I've had trouble trying to find the videos where you take the dna and look at the actual proteins. maybe i will find it soon :)
Thank you very much ma'am.It really helped a lot.
very well explained.... thanx alot for making such video
This helped me so much for my mcat test
Such a good and simply way of explanation thank you
God give me an opportunity to spend 15 mins of my life time in a precious way...
thanks i hope another video release in this related video
Very good presentation
You teach excellent. Thank you.
everything is just so clear, thanks
Well explained. Thanks. Can talk on chromatography in details
Very well explained
This is not my field at all so please excuse my ignorance; Since DNA is a negative molecule (3:48), does that mean it is an ion?
Thank you Ma'am.. It's a great video! ❤
Well presentation
Not sure if the comments are still answered, but... Why do we need a PLATINUM wire electrode in the electrophoresis setup? What exactly will happen if one replaces that electrode with an ordinary Copper wire?
Please upload some videos about difference between RNA and DNA isolation and separation techniques. Do tell about Difference in their agarose gel concentration and gel separating chamber, where we use horizontal or vertical gel chambers...
It was a very nice lecture!
Thanks. Very clear explanation
Very very good explanation
Thank u very much i love all your videos
Hi this video is great! I was wondering if the thickness of the band would suggest any properties about the nucleic acid strand, i.e there are more nucleic acid fragments of that size?
You are great teacher mam
Nice job!
Great video, thanks!
I have a question concerning visualisation: If after the gel we have the blue bands with the loadind dye, why do we need to add Ethidium bromide to make it visible under UV? Isn't the information we have from the blue bands sufficient? Or is it maybe, that the blue bands we see after we ran the gel is maybe almost only the dye itself, as it leaves the DNA (or RNA) and just sinks faster to the + side, so that our actual DNA (or RNA) is located a bit above the dye - and that is why we need to check it under UV, as it would be "invisibly" located above the dye? Really can't find any answer to this! Thanks again! (:
We either add GelRed right into the gel or we use ethidium bromide later for visualization; both are carcinogens but we would not use both at the same time.
Informative
You are the best thank you from my heart 💜
Very informative🥰
Are PCR and then AGE being done in an automated fashion on a single sample in a miniature electrochemical device, such as one that can only be used once ?
Really good explanation !!!!
Thank you very much for this video ma'am
Very informative. I guess it is SYBR Green not cyber green ;)
Ma'am have pronounced rightly.
It is SYBR, pronounced as cyber but correct spelling is SYBR.
Supr class. Thank you🤩🤩
How can one read a plasmid using three different enzymes to cut
Hi! I just found you and I have been watching your videos. They are amazing! I was wondering (this may be a silly question) but how do you know which restriction enzymes to use for example for paternity testing? does it matter which to use as long as all DNA samples are treated the same? thank you! look forward to watching all your videos--Claudia
Hei .. thank you for your comment ... as you said, you should treat all the samples with the same restriction enzyme .. and experimentation will show us which is the best restriction enzyme to use :)
@@biomedicalandbiologicalsci4989 Can we search it out from the literature which endonuclease to be used?
I have a question .. when migrating the genomic DNA .. shouldn't multiple bandits appear, each expressing a single chromosome content .. since chromosomes carry DNA of very different sizes, especially if we are talking about humans, for example
please make some more videos .good explanation
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
Thx, very useful well done
Thank you for your comment ...
Im confused. Why does the solution not evaporate in the microwave?
Thanks a lot, ma'am.
Hi how can I determine the effectiveness if i will alternative coloring dye for the loading buffer
Hi ma'am, could you please talk about PCR and it's various types?
THANKYOU 💞
Make some videos related with r dDNA technology
Thanks a lot
please make a vedio why Taq DNA polymerase is thermostable
Thanx thats was helpfull
you do all :salut
What is the use of nylon fiber?
Thank you
What are amplicons?
Brilliant
Nice mam
Thanks
❤❤❤
like it
Sorry, but there is no such thing as DNA junk sequence anymore.
Replication
I don't think anything in nature and universe is junk or useless
In silico: czcams.com/video/BkTRYMjyatA/video.html
explained very nicely
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
Thank you