GRYA recipe 2% Glutinous Rice/Tapioca/Arrow root/Whole wheat flour 2% Agar 0.1% Baker's or Nutritional Yeast Food Coloring (if desired) FOR 500 ml Total volume 10 g Glutinous Rice/Whole wheat flour 10 g Agar 0.5 g Baker's Yeast Food Coloring or 1g charcoal if desired. 500 ml tap water -Should make between 20-25 standard size plates. Rice Water Agar Boil rice, noodles or pasta with excess water (at least 1.5 liter). Use about 100g of rice/pasta and 1.5 liter of tap water. After boiling the rice for about 30 minutes, use a strainer and bowl to collect the excess water. SAVE THIS WATER! Let it cool. Discard the rice or cook it more for consumption. For 500 ml of cooled rice water, measure out 10g of agar, 0.5g of Baker’s yeast, and add it to the cooled water. Do not add agar to hot liquid. It will clump-up and be very difficult to dissolve. If you are in a hurry, dissolve the agar/yeast in cool water, then add it to the hot water to make the total volume 500 ml. If the rice water is too ‘rich’, you may need to dilute it with clean tap water. Agar media that is too rich will lead to tomentose mycelium that is difficult to differentiate from contamination. Lower nutrient levels tend to grow mycelium that is more rhizomorphic and indicative of dikaryotic P. cubensis mycelium. Swirl this until the agar is mostly dissolved. Pressure cook as normal. Pour 20-25 standard 90 mm plates.
I haven't tried this type yet but I will soon! My Autistic son and I were watching this and he asked me what does KISS stand for? I told him it stands for (Keep It Simple Stupid).. but I'll switch the end to (Silly) for him. So 15 minutes later I hear him down in the kitchen, I go down and ask him what he's doing? He goes making a sandwich KISAH style. I'm like KISAH 🤔? I go you mean KISS style, he said no, this is my version (Keep It Simple AssHole). 😳.... I'm like, you calling me an asshole? he goes that depends if you're keeping it simple or not 😂🤣 Thank you for another great video Ed! You bring a lot of knowledge and fun to my house 🖤
These videos are good. I don't think there can be to many as long as everything is legit or not bad info. I think they help people with anxiety about doing it, seeing someone else do it and explain it. Thanks for the video ED! ❤
Dude I just wanna say thank you! your vids have been so helpful and the diaper tek is genius. I wish you nothing but the best ❤❤ to you and your loved ones!
I don't have any Cordyceps cultures and am not familiar with the techniques. I reckon they are the same procedures used in the videos I have made already.
When I used potato flakes most of my mycelium was Tomentose (I don’t know how to spell it!) lol when I switched to the brown rice flower I got all sorts of rizomorphic growth! Is it the recipe that helps it be rizomorphic?
I would, but the malt extract tends to get clumpy. I also change my recipe constantly, so a pre-mix would be kind of pointless. Changing the media often is a good way to keep the fungi on it's toes and stave off senescence.
@@edwardgrand Thanks and funny you say that. Ironically, the reason I wanted to create some mixes, was to have a range of quick recipe options. Also, thanks a ton for your commentary below on overly rich media. What you described is exactly what I'm dealing with (I think). I'm getting some intense tomentose colonies, and at the same time, and from the same (more or less) genetics, some great looking rhizomorphic plates. I'm not obsessed with rhizo, but it does give me more confidence concerning contamination. Since I've been staring at some of these plates daily and scratching my head, I really appreciate the heads up.
Thanks for another great video, Ed! Maybe you can tell me: What's the big difference between nutritional yeast and yeast extract? Almost every recipe I found on the internet uses the more expensive extract instead of the supermarket yeast.
I know I'm a little late to the party, but do you use any kind of special recipe for spore germination or do you just use whatever is on hand? I know some people put less nutes into their spore germ plates and make the agar softer and whatnot. Just curious what your take on it is
Ed - my Telephone Agar is fungicidal! Got a new shipment Sep23 of the 25g packets and began using those in Dec. Nothing would grow for 2 months as I experimented to track the problem. Agar from a different mfr solved the problem. I have side-by-side plates where the only difference is the TP vs New agar. The myc won't grow onto the TP agar. I'm trying to revive the old transfers now and starting to see life; was worried all were dead after that long on anti-fungal agar. I starting using a capped media bottle in PC and let the boil mix the ingredients about the same time. Maybe that traps deadly volatiles that could otherwise escape in a simmering pan? Or else TP changed something in the packets. I haven't experimented to answer this yet. The TP also would not set hard. Increased to 12g in 500ml and still would not set harder than toothpaste.
Sorry, that doesn't make any sense. Maybe one of your containers has residual chemicals in it? Real Telephone brand is distributed worldwide and commonly used for culinary purpose. I doubt it is the brand's fault. 12g for 500 ml not setting. Very weird. You are doing something wrong. Don't blame it on the agar.
I'd rather not blame the agar but that is where I'm at, until I have the time to run a side-by-side 1 factor experiment. The only things that touch or go near my agar are: Glass petri dishes (new to me but others from same batch have grew ok in the past) Glass Media Bottles (was bought new about the same time as the problem started, but worked fine with new agar) Stir bar Telephone Agar (was a new batch when problems started) Light malt extract (same old batch for 2 years now) Flowbench Grafting tape wrap (new, works with new agar) 77F incubator (used before with success) All of the above were used for TP agar batches that failed. Then same again for 1 FitLane agar batch that is growing. But the final word would be to mix up batches of the agars at the same time, and through the same PC run, and pour every-other petri at the same time, then do side-by-side clone samples or agar transfers. Besides 12g not setting hard the other oddity was 0% contamination of any TP plate during these months. Is there a better format to communicate with you, one of the discords, or other ? Don't want to talk your ear off. @@edwardgrand
GRYA recipe
2% Glutinous Rice/Tapioca/Arrow root/Whole wheat flour
2% Agar
0.1% Baker's or Nutritional Yeast
Food Coloring (if desired)
FOR 500 ml Total volume
10 g Glutinous Rice/Whole wheat flour
10 g Agar
0.5 g Baker's Yeast
Food Coloring or 1g charcoal if desired.
500 ml tap water
-Should make between 20-25 standard size plates.
Rice Water Agar
Boil rice, noodles or pasta with excess water (at least 1.5 liter).
Use about 100g of rice/pasta and 1.5 liter of tap water.
After boiling the rice for about 30 minutes, use a strainer and bowl to collect the excess water. SAVE THIS WATER! Let it cool. Discard the rice or cook it more for consumption.
For 500 ml of cooled rice water, measure out 10g of agar, 0.5g of Baker’s yeast, and add it to the cooled water. Do not add agar to hot liquid. It will clump-up and be very difficult to dissolve. If you are in a hurry, dissolve the agar/yeast in cool water, then add it to the hot water to make the total volume 500 ml.
If the rice water is too ‘rich’, you may need to dilute it with clean tap water. Agar media that is too rich will lead to tomentose mycelium that is difficult to differentiate from contamination. Lower nutrient levels tend to grow mycelium that is more rhizomorphic and indicative of dikaryotic P. cubensis mycelium.
Swirl this until the agar is mostly dissolved.
Pressure cook as normal.
Pour 20-25 standard 90 mm plates.
Your are the Man! Thanks
Do you ever add Peptone to your agar recipes? I often see it as an ingredient in LC. I know less is more with agar but I just can't help me self lol
@@smittyhaggis6283 no
Have you ever used sweet rice flour? Like the stuff they use to make mochi out of? Would it work well or do you think it would it be too sweet?
@johnhauser5939 yes. It works great.
Thank you for sharing your wisdom and knowledge Ed!
I haven't tried this type yet but I will soon!
My Autistic son and I were watching this and he asked me what does KISS stand for?
I told him it stands for (Keep It Simple Stupid).. but I'll switch the end to (Silly) for him.
So 15 minutes later I hear him down in the kitchen, I go down and ask him what he's doing?
He goes making a sandwich KISAH style. I'm like KISAH 🤔?
I go you mean KISS style, he said no, this is my version (Keep It Simple AssHole). 😳....
I'm like, you calling me an asshole? he goes that depends if you're keeping it simple or not 😂🤣
Thank you for another great video Ed! You bring a lot of knowledge and fun to my house 🖤
I've tried it all and the best and the easiest recipe has definitely been:
For 500ml:
10g Agar
6.5g (Extra) Light Malt Extract
1g Nutritional Yeast
i do mine with just 1.5% instant potatoe powder and 1.5% water.
i had activated charcoal when i feel fancy.
I was watching the green eggs in the background but see no ham🤔
Hello Ed! Glad to see a new vid!
🎶 doo do doo - the last thing you hear before Ed drops some knowledge on yo ass
These videos are good. I don't think there can be to many as long as everything is legit or not bad info. I think they help people with anxiety about doing it, seeing someone else do it and explain it. Thanks for the video ED! ❤
Dude I just wanna say thank you! your vids have been so helpful and the diaper tek is genius. I wish you nothing but the best ❤❤ to you and your loved ones!
Thank you very much for the message. I'm very happy the videos are helpful.
Thanks for sharing 👍
Great as usually. Ed could you please make a video on a simple method to breed Cordyceps Militaris? I know wrong forum but I am lost.
I don't have any Cordyceps cultures and am not familiar with the techniques. I reckon they are the same procedures used in the videos I have made already.
Try Renegade Mushrooms
Excellent content 👍
I always k.i.s.s my agar, Mush Love!
Man those limes are perfect 🤩 hey Ed thanks for another Slapping ass videos !!!!!
When I used potato flakes most of my mycelium was Tomentose (I don’t know how to spell it!) lol when I switched to the brown rice flower I got all sorts of rizomorphic growth! Is it the recipe that helps it be rizomorphic?
Yes. The recipe dramatically affects growth style. It is irrelevant to the final fruit product as long as it's a clean culture.
@@edwardgrand ahhhh ok thanks Ed
Ed do you have a video showing how you do LC? I don’t see it or am I missing the video?
Yes. I have several videos using that technique.
Thanks man Doc! Was wondering if you ever premix some of your dry ingredients and store them for later use (weeks or months later). Thx!
I would, but the malt extract tends to get clumpy.
I also change my recipe constantly, so a pre-mix would be kind of pointless. Changing the media often is a good way to keep the fungi on it's toes and stave off senescence.
@@edwardgrand Thanks and funny you say that. Ironically, the reason I wanted to create some mixes, was to have a range of quick recipe options. Also, thanks a ton for your commentary below on overly rich media. What you described is exactly what I'm dealing with (I think). I'm getting some intense tomentose colonies, and at the same time, and from the same (more or less) genetics, some great looking rhizomorphic plates. I'm not obsessed with rhizo, but it does give me more confidence concerning contamination. Since I've been staring at some of these plates daily and scratching my head, I really appreciate the heads up.
Definitely try easing back on the nutrients. Especially the carbohydrate source.@@MacCelium
🎉😊
Thanks for another great video, Ed! Maybe you can tell me: What's the big difference between nutritional yeast and yeast extract? Almost every recipe I found on the internet uses the more expensive extract instead of the supermarket yeast.
Cost and final appearance. If you use Baker's yeast, there will be small chunks in media. I haven't noticed any difference in growth.
that's my go to saying ;)
I know I'm a little late to the party, but do you use any kind of special recipe for spore germination or do you just use whatever is on hand? I know some people put less nutes into their spore germ plates and make the agar softer and whatnot. Just curious what your take on it is
No special recipe.
Thanks, Ed! You're my go to for just about everything when it comes to mycology these days
😘
hmm, this makes sense idk y i didnt try
lol keep it simple… starts video talking about all sorts of starches I’ve never heard of using 😂
Google and Wiki brother. If you're not here to learn, pick another hobby.
Ed - my Telephone Agar is fungicidal!
Got a new shipment Sep23 of the 25g packets and began using those in Dec. Nothing would grow for 2 months as I experimented to track the problem.
Agar from a different mfr solved the problem. I have side-by-side plates where the only difference is the TP vs New agar. The myc won't grow onto the TP agar. I'm trying to revive the old transfers now and starting to see life; was worried all were dead after that long on anti-fungal agar.
I starting using a capped media bottle in PC and let the boil mix the ingredients about the same time. Maybe that traps deadly volatiles that could otherwise escape in a simmering pan? Or else TP changed something in the packets. I haven't experimented to answer this yet. The TP also would not set hard. Increased to 12g in 500ml and still would not set harder than toothpaste.
Sorry, that doesn't make any sense. Maybe one of your containers has residual chemicals in it? Real Telephone brand is distributed worldwide and commonly used for culinary purpose. I doubt it is the brand's fault. 12g for 500 ml not setting. Very weird. You are doing something wrong. Don't blame it on the agar.
I'd rather not blame the agar but that is where I'm at, until I have the time to run a side-by-side 1 factor experiment.
The only things that touch or go near my agar are:
Glass petri dishes (new to me but others from same batch have grew ok in the past)
Glass Media Bottles (was bought new about the same time as the problem started, but worked fine with new agar)
Stir bar
Telephone Agar (was a new batch when problems started)
Light malt extract (same old batch for 2 years now)
Flowbench
Grafting tape wrap (new, works with new agar)
77F incubator (used before with success)
All of the above were used for TP agar batches that failed.
Then same again for 1 FitLane agar batch that is growing.
But the final word would be to mix up batches of the agars at the same time, and through the same PC run, and pour every-other petri at the same time, then do side-by-side clone samples or agar transfers.
Besides 12g not setting hard the other oddity was 0% contamination of any TP plate during these months.
Is there a better format to communicate with you, one of the discords, or other ? Don't want to talk your ear off.
@@edwardgrand
I think ill start using some yeast in my mix again.