Damn, Ed, I'm learning so much from you. Problem is, at my age, I have difficulty remembering everything. But I can always rewatch your vids and learn it all over again for the first time. 🤣 Thanks for sharing your work. Peace
Since finding you channel a month ago ive made a few changes to my methods. Ive moved on from "No Pour Agar Cups" to now pouring Petri dishes in my SAB with great success! The dishes are wrapped in Grafting Tape (that stuff is awesome) and i have practiced a "Grab and Drag" on an Averys Swab last week. It worked really well and had a few spots of Mycelium on the drag channel. I then realised i have no microscope and am in no position to play with monos yet 😂 i am letting them grow a bit more before transferring to new plates to hopefully avoid getting a mono. Hepa filter and microscope are next on the "to buy list". Thanks for the education and inspiration Ed, hope you are well.
I'm so glad I inspired you. The more we have the potential to do good things with Mycology, the easier those purchases are to justify :) The wish list changes gradually over the years. I'm thinking about a pressure cooker now that costs more than my first two cars...LOL
Hey Ed, learning something everyday from your channel! I got a question re transferring potential monos from a grab&drag plate.. Is there a correlation between sample size and risks of contam? As in the smaller the piece the higher the contam risk? I did 5 tentative mono (tiny) transfers from my G&D plate, to separate plates, as well as 4 MS (bigger) transfers from the same plate. Most of the tiny potential mono transfers developed a shiny blobby contam before any mycelium started growing, whereas the bigger MS transfers are doing just fine.. all from the same plate. Is there a logic behind this? I use MYA btw.
Your thoughts on how long a HEPA filter is good for? I found one from 1990 that seems to be in pretty good shape. How would you test with petri placement and length of exposure?
Hi Ed, James from Argentina here! I have one question regarding what microscope should I get to check. Clamp connections?. I can get a hold of a SV605 40x-1600x? Thank you in advance!
I just took a look. That SV605 should work fine. 400X is for checking for clamps, spores on fruit, and general morphology. 1000X is for basidia and other fine features.
QQQ-hey ed! hope all is good, this may be a silly question, but what is the best way to store the rest of the Q-tip for further use after you’ve completed a grab’n’drag
Time/plate saving tip: if you have two confirmed monos, just throw them in the same grain spawn bag. They will dikaryotize in the bag and the new dikaryon will fruit. Monos don't fruit. I have found nearly 100% compatibility among monokaryons. All that talk/gossip/folklore/misinformation about mating/pheromone alleles just isn't true with cubes. You can just clone a nice F1 fruit for further runs and get spores from the same fruit to start the F2 generation.
So when you say drag, you mean drag.You don't mean rabbit lightly whisper round.You mean take that son of a bit?Ch put it down and pull it down so you need some pretty Solid egar compared to the usual softer stuff.I use for spores?
@@edwardgrand okay, i usually use 9g's per 500ML for agar I intend to germinate in. I have tried this method a few times, I am trying to ghetto cross two species with fairly distinctive mycelium - which only helps me distinguish if I have one or the other, Im not sure what a cross would look like - I switched it up and made a couple streaks one way, and the other strain perpendicular, hoping at the intersection to get a cross - we'll see if it works out, guess you dont know until you fruit huh? on a side note, I have a melmak x stargazer cross that i believe originally came from you (I have affectionately named it "Peter Gazer") - it has been kind of a B to get some hearty Rhyzo growth out of, I just put some to grain, is it a fairly stable cross? do you remember how it fruits?
@@etownKeystoned Two distinct species will be hard/impossible to cross. That would be like mating a cat and dog. Mycelium morphology is very highly dependent on media formulation. Good luck with that :) Like you said, you don't know until you try. Lower your nutrients to favor rhizomorphic growth. There are photos somewhere on my FB. Don't recall what it looked like now.
Putting the pieces together, Thanks Ed!
Ed, you are a gift to humanity.
Awe yeah
Damn, Ed, I'm learning so much from you. Problem is, at my age, I have difficulty remembering everything. But I can always rewatch your vids and learn it all over again for the first time. 🤣 Thanks for sharing your work. Peace
Helpful info 👍
Since finding you channel a month ago ive made a few changes to my methods.
Ive moved on from "No Pour Agar Cups" to now pouring Petri dishes in my SAB with great success!
The dishes are wrapped in Grafting Tape (that stuff is awesome) and i have practiced a "Grab and Drag" on an Averys Swab last week.
It worked really well and had a few spots of Mycelium on the drag channel.
I then realised i have no microscope and am in no position to play with monos yet 😂 i am letting them grow a bit more before transferring to new plates to hopefully avoid getting a mono.
Hepa filter and microscope are next on the "to buy list".
Thanks for the education and inspiration Ed, hope you are well.
I'm so glad I inspired you. The more we have the potential to do good things with Mycology, the easier those purchases are to justify :) The wish list changes gradually over the years. I'm thinking about a pressure cooker now that costs more than my first two cars...LOL
O shit I was just looking for a video! 🍄❤️ Thanks bro! Hope you have a good day 😊
Hell yeah doc keep it up brotha
Hey Ed, learning something everyday from your channel!
I got a question re transferring potential monos from a grab&drag plate.. Is there a correlation between sample size and risks of contam? As in the smaller the piece the higher the contam risk?
I did 5 tentative mono (tiny) transfers from my G&D plate, to separate plates, as well as 4 MS (bigger) transfers from the same plate. Most of the tiny potential mono transfers developed a shiny blobby contam before any mycelium started growing, whereas the bigger MS transfers are doing just fine.. all from the same plate.
Is there a logic behind this? I use MYA btw.
I championed for "The Grand Drag" but Grab'n'Drag won out
That's okay. The Grand Drag might get confused with one of the 'special' shows they have here!
Your thoughts on how long a HEPA filter is good for? I found one from 1990 that seems to be in pretty good shape. How would you test with petri placement and length of exposure?
I would leave a few plates open at various places in front of it for 30 minutes. Incubate for 7 days and check.
Hi Ed, James from Argentina here! I have one question regarding what microscope should I get to check. Clamp connections?. I can get a hold of a SV605 40x-1600x? Thank you in advance!
I just took a look. That SV605 should work fine. 400X is for checking for clamps, spores on fruit, and general morphology. 1000X is for basidia and other fine features.
@@edwardgrandThank you a lot! I really appreciate the knowledge your sharing man.
Cheers
QQQ-hey ed! hope all is good, this may be a silly question, but what is the best way to store the rest of the Q-tip for further use after you’ve completed a grab’n’drag
Just put it back in the sleeve/envelope and store at room temp.
So when your monokaryon's touch, do they turn dikaryotic at the point of contact, or does it rapidly spread across the plate making clamps?
No. But the new dikaryon can overgrow the existing monokaryons, making it appear so.
Time/plate saving tip: if you have two confirmed monos, just throw them in the same grain spawn bag. They will dikaryotize in the bag and the new dikaryon will fruit. Monos don't fruit. I have found nearly 100% compatibility among monokaryons. All that talk/gossip/folklore/misinformation about mating/pheromone alleles just isn't true with cubes. You can just clone a nice F1 fruit for further runs and get spores from the same fruit to start the F2 generation.
Nice watch me weed wack I swear you’ll like it
Monokaryon what is it?
Haploid gamete
So when you say drag, you mean drag.You don't mean rabbit lightly whisper round.You mean take that son of a bit?Ch put it down and pull it down so you need some pretty Solid egar compared to the usual softer stuff.I use for spores?
You literally answered that question right after I typed that so I guess I should be patient
@@etownKeystoned It works better if you use 1.5% agar. 2.0% tends to rip and tear.
@@edwardgrand okay, i usually use 9g's per 500ML for agar I intend to germinate in. I have tried this method a few times, I am trying to ghetto cross two species with fairly distinctive mycelium - which only helps me distinguish if I have one or the other, Im not sure what a cross would look like - I switched it up and made a couple streaks one way, and the other strain perpendicular, hoping at the intersection to get a cross - we'll see if it works out, guess you dont know until you fruit huh?
on a side note, I have a melmak x stargazer cross that i believe originally came from you (I have affectionately named it "Peter Gazer") - it has been kind of a B to get some hearty Rhyzo growth out of, I just put some to grain, is it a fairly stable cross? do you remember how it fruits?
@@etownKeystoned Two distinct species will be hard/impossible to cross. That would be like mating a cat and dog.
Mycelium morphology is very highly dependent on media formulation. Good luck with that :)
Like you said, you don't know until you try.
Lower your nutrients to favor rhizomorphic growth.
There are photos somewhere on my FB. Don't recall what it looked like now.
@@edwardgrand sorry, STRAINS not species