Pipetting Technique | Good Pipetting Practice | GPP

Learn how to reduce pipetting errors and ensure data quality with good pipetting techniques from Rainin. Differentiate correct from incorrect ways of holding a pipette and find out how to pre-rinse, aspirate, and dispense. Learn the most ergonomic practices to maximize reproducibility while protecting your hand, wrist, and arm.
Interested in an in-person Good Pipetting Practice seminar? Our GPP experts will train you and your team right in your lab:
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Enjoy this brief playlist of short, pipette technique videos:
• Rainin pipette trainin...

[@bubblesxo]

we love 30 minute videos about a pipette!

[@METTLERTOLEDOLaboratory]

Thank you for watching!

[@khalilmomo718]

most comprehensive video on pipetting I've ever come across. thanks!

[@METTLERTOLEDOLaboratory]

Wow, thanks!

[@santoshduddelly4988]

Excellent learnt many things though using pipette for 10yrs

[@METTLERTOLEDOLaboratory]

Thanks for watching!

[@cjjones1347]

Thank you for the good techniques!

[@METTLERTOLEDOLaboratory]

Thank you for watching it

[@snipelessTS5]

Very informative and helpful thank you!

[@METTLERTOLEDOLaboratory]

Glad it was helpful!

[@rebecab.737]

Lovely!

[@alanacevedo4928]

Thank you for this video!

[@METTLERTOLEDOLaboratory]

Glad it was helpful!

[@SaadSaad-mb4py]

Thank you very much

[@METTLERTOLEDOLaboratory]

You are welcome

[@timalysheva]

Thank you- very informative

[@METTLERTOLEDOLaboratory]

Thank you for watching!

[@SaadSaad-mb4py]

Good very good

[@METTLERTOLEDOLaboratory]

Thank you for watching!

[@SaadSaad-mb4py]

Thank you very good

[@METTLERTOLEDOLaboratory]

Thank you for watching!

[@jenniferguaman2364]

When you want to release volume into a vial.. you mentioned how you must first go to the first stop than hold ? When you say hold do you mean still have your finger held in place in the first stop ? Then you again until you hit the 2nd stop ?

[@METTLERTOLEDOLaboratory]

Yes: Stop at the first stop. That delivers the full volume you set on the pipette. You will see a tiny amount of residual liquid left inside the pipette tip. To empty the tip completely, move the pipette over a different container then press the plunger all the way down.

[@francescobaldi8876]

@@METTLERTOLEDOLaboratory wow.. now I understand why my calibration curves always have a low R^2 😅

[@yassie449362]

how would the apa citation of this video be?

[@ninjabrown8560]

you shouldn't really cite videos in scientific work, annoying I know but still x

[@METTLERTOLEDOLaboratory]

You could cite this video the following way: Rainin, Laboratory Solutions from METTLER TOLEDO, 24 October 2014, Good Pipetting Techniques from Rainin, [video], CZcams.com, czcams.com/video/X73R_4cntpA/video.html

[@syed-wd8ib]

How can we use dial down technique for 100% desired volume

[@METTLERTOLEDOLaboratory]

Dial above the pipette's maximum volume, then dial down from there. For example, on a 200 µL pipette, dial to ~205 µL then dial down to set. The pipette will allow you to do this.

[@SaadSaad-mb4py]

Fantastic

[@METTLERTOLEDOLaboratory]

Thank you for watching!

[@cheyenneautumn7658]

Can I use universal pipette tips on a pos d pipette?

[@METTLERTOLEDOLaboratory]

No. Pos-D has its own tips, sometimes called "capillaries" and sometimes called "syringe tips." Each Pos-D tip has its own built-in plastic piston. The little metal "jaws" inside a Pos-D grab the top of that inner plastic piston to move it up and down. It's a different mechanism from regular air-displacement pipettes that use universal tips. Pos-D is positive displacement: no air between the sample and the end of the piston inside the Pos-D tip.

[@leraniemackl2710]

most comprehensive video on pipetting I've ever come across. thanks!

[@METTLERTOLEDOLaboratory]

Glad it was helpful!