Great job man! Huge respect! If you're active help me to clarify these doubts: 1. What does 30M sequencing depth mean in RNA-seq? Does it mean that those fragmented cDNAs are sequenced 30M times? Why it is required to sequence this much? In DNA seq, 30x coverage means each nucleotide is sequenced 30 times. How does it differ from DNA coverage? Many Thanks !!
Is possible to balance the gene pool as it is very difficult to balance gene expression. So some coverage is vary sample to sample. How we can balance gene expression?
clearest explanation about RNA-seq in youtube, thanks a lot bro!
Why the viewers of this video just 13K? This is insane!! Thank u buddy .. You are my savior!
Great job on explaining the concepts !! Please make more videos on RNA seq. Thanks,
Neima !!
This was really concisely and clearly explained, thank you!
Glad you enjoyed it! :-)
jeepers you're a rockstar in my eyes
GREAT EXPLANATION THANK YOU SO MUCH
Great job man! Huge respect!
If you're active help me to clarify these doubts:
1. What does 30M sequencing depth mean in RNA-seq? Does it mean that those fragmented cDNAs are sequenced 30M times? Why it is required to sequence this much?
In DNA seq, 30x coverage means each nucleotide is sequenced 30 times.
How does it differ from DNA coverage? Many Thanks !!
Thank you for the video!
Are those videos part of an online course?
Is possible to balance the gene pool as it is very difficult to balance gene expression. So some coverage is vary sample to sample.
How we can balance gene expression?
How to calculate p-value for each gene under 2 conditions?
do the reads come from cluster generation?
The reads are generated using an RNA-seq protocol, such as the one developed by Illumina: czcams.com/video/AhKOiP9wbc8/video.html