Perfusion bioreactors
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- čas přidán 28. 08. 2024
- Perfusion is a continuous culturing method in which cells are either retained in the bioreactor or fed back into it using a cell retention device. The harvested medium thus contains no cells, resulting in higher cell concentrations and product yields in the reactor while still reducing the working volume. This technique is increasing in popularity with a general shift of bioreactors towards smaller continuous reactors. In this video, I will discuss opportunities for this method but also drawbacks of this emerging technology.
For more videos on this topic, check out our Bioreactors playlist or the playlist Topical research.
Thank you for your videos on bioreactors. They've been very helpful in designing my own simplified system to grow phytoplankton to feed to my aquarium
Great to hear! Hope your phytoplankton is thriving :)
@@MarloesPeeters I haven't started my culture just yet. I'm planning to start it up this week though. I'm basically building the most simplified chemostat bioreactor I can. It's just going to be sterilized saltwater with f2 fertilizer fed into a reaction chamber using a dosing pump and then overflowing into a collection cup. I really appreciate the words of encouragement and hope you have a great day
Excellent video. Thank you! ☺️
You are so welcome! Let me know if you have any suggestions about other videos on bioreactors
helped me understand this sooo much
Glad to hear!
Very good video. Thank you.
Thanks for the suggestion to make a video on this topic :)
Thank you. Good video.
Glad you liked it!
Thanks for the interesting video. I'd like to know what do you mean by saying "the perfusion bioreactors are not well characterized like batch bioreactors"?
Dear Vida, thanks for your question. What I mean is that traditional batch bioreactors have been used for much longer and therefore there is a better understanding of how they work. Perfusion bioreactors are more of an emerging technology which only over the last year are gaining in popularity due to scientific advances in for instance membrane technology and cell retention systems. Hope that answers your question.
@@MarloesPeeters Thanks for the clarification!
Thank you so much
Thank you! Are there any particular bioreactor topics you would like to learn more about?
Hi, can I use the schematic of the perfusion setup at 1:32 for an assigment? Thank you
The source of this schematic can be found here: pubmed.ncbi.nlm.nih.gov/30024096/ - you can use these as long as you reference them
Very helpful video! Do you know where I can look to learn more about perfusion with yeast and filamentous fungi?
That is a good question! I am more familiar with mammalian culture in perfusion reactors - will have to look into this further
@@MarloesPeeters Amazing! Thank you for being so open and accessible. For context, I'm working on a business plan for food production via fermentation and I think perfusion has great potential to make the technology scalable. Looking forward to finding out what you think after looking into this!
I have not been able to find that much. Since yeast is easily grown into a stirred tank reactor, I guess the real question is what makes the perfusion reactor better. You can go to higher cell densities so it might be more efficient, but the real crux is a) a good cell retention device b) how to source the media. Filamentous fungi are more sensitive to shear so there is more of a rationale for moving away from a stirred tank reactor but why not an airlift reactor for instance. I could mainly find articles on cell settlers or retention devices and very little on for instance yeast. I have found some micro-scale perfusion-based systems for yeast such as here: onlinelibrary.wiley.com/doi/full/10.1002/biot.202000215, but not on large scale