Zeiss LSM800 Airyscan confocal tutorial

This video covers the hardware and software for Newcastle University's Bioimaging Unit LSM800 confocal, including startup, shutdown, and operating the software. Details and bookmarks for sections below:
Startup
00:16 - System Startup
01:52 - Using an Oil Immersion Objective
02:50 - Starting Zen and a quick overview
03:50 - Using the Oculars
04:13 - Using the camera to check your sample
Basic channel Setup
05:00 - Setting up for confocal imaging with Smart Setup
07:15 - Describing Tracks and Channels and manual adjustment of Imaging Setup
08:00 - Line and Frame mode differences
09:03 - Detectors on System
09:35 - Fine tuning Imaging Setup manually
11:14- Going Live and using the histogram
11:54 - Using bidrectional scanning
12:15 - Using Nyquist sampling and changing frame size in Acquisition Mode
13:17 - Setting Gain and laser power using the histogram
14:18 - Setting Gain and laser power using the Range indicator
14:38 - 8 bit and 16 bit output
15:05 - Setting gain and laser on a 2 channel Track
15:38 - Taking a Snap Image
16:10 - checking the image for fine tuning
16:54 - Check focal planes for final image
17:34 - Checking for bleedthrough within a multichannel Track
18:40 - Improving Image Quality with dwell time
20:00 - Improving Image Quality with Averaging
Zed Stacks
20:47 - Taking Zed Stacks using mark Centre
22:01 - zed sampling frequency
23:10 - Using Start Experiment
23:30 - Viewing 3D images
24:19 - Setting Zed Stack with First and Last
Tiling and Multipositions
25:05 - Tiles and Positions setup
26:30 - Using confocal Zoom for overview
26:45 - Taking a Confocal TileScan
27:45 - Taking overview scans using Widefield Imaging
30:15 - Setting Multiple positions from an Overview Scan
31:19 - Checked zed positions for multiposition imaging with Verify Positions
32:14 - Navigating through multiposition czi ifiles
32:30 - Advanced Tiles setup for Preview Scans
34:22 - Setting Support Points for tiled images
36:09 - Focussing Strategy options
Confocal and Airyscan imaging
37:38 - Using Confocal Zoom to take cropped images
38:30 - Nyquist confocal Imaging
38:54 - Using Smart Setup to create Airyscan setup
40:40 - comparing Airyscan to Confocal imaging
41:19 - checking Airyscan alignment
Timelapse with Mulitpositions
41:50 - moving the stage via captured images to set up multipositional imaging
42:56 - Setting multiposition, multitime imaging
43:26 - Navigating through time series multiposition czi files
43:56 - Setting incubation conditions
Saving and Shutting down
44:56 - Saving your images
45:46 - Shutting down Zen
46:02 - Copying data to server
Hardware Shutdown
47:05 - Shutting down the hardware
47:22 - Cleaning oil immersion lenses after use

[@n-sh2014]

Such a fabulous and informative tutorial video. Thanks a million!

[@vasilikiskiada2332]

Hello. Thank you for this detailed tutorial. I would like to perform imaging of a fluorochrome and on the same time record the transmitted light image. Is there a way to do that? Thank you.