Hi, It's amazing to learn from your vedio demonstration. I wonder this silica gel slurry is made by chloroform/methanol solvent or acids? Slurry is thick. Thank you
This is a terrible instructional video. You need to add sand on top of the cotton plug so the silica is a complete tube and not tapered at the bottom and also the tube is packed to high and again without sand on the top to stop your sample from disturbing the surface of the silica....which again is packed way to high. You dont want to add silica higher than 5-7 inches and if you need more volume you get a bigger column that has a bigger diameter to put more silica. Not to mention you let the silica run dry which is a big no no and you learn that the first day of column chromatography. aaaaaanywaaaaays....
Ethyl acetate is a polar solvent. Normal hexane is a non-polar solvent. Technically this is a mixture of both. The ethyl acetate is a minority addition though. Sometimes solvent based chromatography has overlapping transitions between different compounds and adding extra bits, as long as they are of known composition, helps sharpen those transitions. I would imagine there's something specific the students are looking for that can become a giant smear without something to keep everything crisp.
great trick to use just a uL of each t-tube fraction and let the spot darken or not tell you yes or no.
what a time saver.
I’ve never seen chromatography done in this way. I’m used to HPLC being performed in a machine with chromatograms. Interesting.
Useful &gained knowledge
Hi, It's amazing to learn from your vedio demonstration. I wonder this silica gel slurry is made by chloroform/methanol solvent or acids? Slurry is thick. Thank you
Thank you.please how many gram of siluca gel to sample
This is a terrible instructional video. You need to add sand on top of the cotton plug so the silica is a complete tube and not tapered at the bottom and also the tube is packed to high and again without sand on the top to stop your sample from disturbing the surface of the silica....which again is packed way to high. You dont want to add silica higher than 5-7 inches and if you need more volume you get a bigger column that has a bigger diameter to put more silica. Not to mention you let the silica run dry which is a big no no and you learn that the first day of column chromatography. aaaaaanywaaaaays....
Hi, can I know what type of sand we need to put there?
Could you tell me what reagent you stain the chromatograph?
very great tutorial!!!!!
My biggest concern is how did you get that cotton plug accurately down there (2:16)? Can someone please help me in that?
Just push it with a rod or something long/thin enough to go down there..
I don't blame you... A good laboratory must always be clean... Take the experiment to "The Hood"...[LOL]
no shit it's gonna trap air if you add silica to column without solvent already in the column
This video HAS to be a joke
@@kcore9688 Why does it _HAVE_ to be a joke?
Do you have a protocol you can share?
11:50 what did you stain the plate with?
PMA
Our sample is dissolving in polar solvents or non polar solvents
Ethyl acetate is a polar solvent.
Normal hexane is a non-polar solvent.
Technically this is a mixture of both.
The ethyl acetate is a minority addition though.
Sometimes solvent based chromatography has overlapping transitions between different compounds and adding extra bits, as long as they are of known composition, helps sharpen those transitions.
I would imagine there's something specific the students are looking for that can become a giant smear without something to keep everything crisp.
Sir what is the staining solution?
Where is the sand? Why aren't they wearing lab coats? Why is there diet coke in the background in the lab?
+Helen Coulshed im assuming no sand because the cotton wool is around 8 micron on its own and is filling the whole taper of the column on its own?