Dialysis (Protein Purification)

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  • čas přidán 29. 08. 2024

Komentáře • 31

  • @oumaymabalti1019
    @oumaymabalti1019 Před 6 lety +11

    in College,we study chemistry and biochemistry in French ,although I speak 3 other languages than english including french,I never liked biochemistry the way I enjoyed your lectures! I wish you were one of my professors! bravo!

  • @chinmay2297
    @chinmay2297 Před 4 lety +1

    We can also separate the protein from the ions in the water by using electrolytes which is oppositely charged to the ions present in the bag and also we can use electrodes setup.

  • @harisbinfida6624
    @harisbinfida6624 Před 8 lety +1

    im a great fan of yours...what a dedicated way to give knowledge to students..really impressed...really appreciating

  • @navneetmishra3989
    @navneetmishra3989 Před 2 lety

    One of the best professor, I have seen love u from India sir 😍😘

  • @nareshbarik5384
    @nareshbarik5384 Před 6 měsíci +1

    Thank you ❤🙏

  • @sameirbasnet3102
    @sameirbasnet3102 Před 8 lety +2

    great lecture will you please explain the process of purification of HIS-tagged protein purification process.

  • @gaymo69
    @gaymo69 Před 7 lety +4

    how come water molecules dont pass through the membrane when held in open air? (Or do they leak out too?)
    Googled answer for myself: Surface tension of water in air keeps it from leaking out the small pores.

  • @arunkumars6257
    @arunkumars6257 Před rokem

    Thank you so much!

  • @jhonamaysuero8568
    @jhonamaysuero8568 Před 8 lety +1

    thanks soo much well explained

  • @shivanipopli6868
    @shivanipopli6868 Před 8 lety

    sir, you are amazing.. thank you so much.

  • @anurag6205
    @anurag6205 Před 7 lety +1

    Thank you sir

  • @leeschmalz6365
    @leeschmalz6365 Před 4 lety

    You're great, thank you

  • @PhilipAlabiephraim
    @PhilipAlabiephraim Před 8 lety

    Question: How is the salting out concentration calculated? because that will be very important in helping get proteins

  • @KoolViking3
    @KoolViking3 Před 8 lety

    awesome!

  • @deepikadav
    @deepikadav Před 8 lety

    can't thank you enough, Sir :)

  • @junczhang
    @junczhang Před 8 lety

    awesome

  • @gosia1761
    @gosia1761 Před 6 lety

    Hello, I really appreciate yours videos, thank you for all of them. I have a question, I want to know why the dialysis bag will be bigger after the dialysis. There's also something from the baker going to the bag. What is it and why ?
    I hope you will give me a response.

    • @mpraneeth1978
      @mpraneeth1978 Před 5 lety

      It's the water molecules diffusing from the beaker - the region of higher concentration- into the dialysis bag which is the region of lower concentration.

  • @tyvenghout9049
    @tyvenghout9049 Před 5 lety

    Can we calculate the concentration at the equilibrium?

  • @dalbir435
    @dalbir435 Před 7 lety

    does dialysis would help to separate dna from proteins?

  • @KJKP
    @KJKP Před 8 lety +1

    I think diffusion is not covered by the 2nd law of thermodynamics. "Every energy transfer or transformation increases the entropy of the universe."

    • @TheYz250fmf
      @TheYz250fmf Před 8 lety +2

      Thermodynamics is everything. You are reading the definition to close and not applying it correctly. Molecules want to increase their entropy correct? (yes) Therefore a molecule will diffuse to increase its entropy and it will be happy.

    • @AKLECTURES
      @AKLECTURES  Před 8 lety +4

      Thermodynamics IS in fact everything :)

    • @TheYz250fmf
      @TheYz250fmf Před 8 lety

      (:

    • @fuhrer12345
      @fuhrer12345 Před 6 lety

      Your explanation is a little superficial. In fact when we deal with an open system such a human being, is quite the opposite. Most biochemical reactions in our body are indeed unfavorable (supramolecular complex), thus they tend to decrease the internal entropy. It's by reaction coupling and non covalent interactions that the molecules in our system can increase the universe's entropy. Gibbs free energy is a good measurement for this phenomenon.

    • @jayrextong3409
      @jayrextong3409 Před 3 lety

      nadali mo hahaha ano imik hahaha nyenyenye

  • @jayrextong3409
    @jayrextong3409 Před 3 lety

    is it like the teabag???? nye nye nye

  • @zanzan648
    @zanzan648 Před 6 lety

    How many type. Of dialysis

  • @wirtsleg
    @wirtsleg Před 6 lety

    Why do cells have to use ion channels to transport the ions through the membrane but here it isn't needed? Why can't the cells also use the 2nd law of thermodynamics to have equal amounts of ion inside and outside the cell membrane?

  • @Halum11
    @Halum11 Před 4 lety

    diceperse...

  • @AhmedAli-bx1vj
    @AhmedAli-bx1vj Před 7 lety

    i love you hhhhhhhhhhhhhhhhhhhhh