PI3k/AKT/mTOR Pathway

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The Akt signaling pathway or PI3K-Akt signaling pathway is a signal transduction pathway that promotes survival and growth in response to extracellular signals. Key proteins involved are PI3K (phosphatidylinositol 3-kinase) and Akt (protein kinase B).
Initial stimulation by one of the growth factors causes activation of a cell surface receptor and phosphorylation of PI3K. Activated PI3K then phosphorylates lipids on the plasma membrane, forming second messenger phosphatidylinositol (3,4,5)-trisphosphate (PIP3). Akt, a serine/threonine kinase, is recruited to the membrane by interaction with these phosphoinositide docking sites, so that it can be fully activated.Activated Akt mediates downstream responses, including cell survival, growth, proliferation, cell migration and angiogenesis, by phosphorylating a range of intracellular proteins. The pathway is present in all cells of higher eukaryotes and is highly conserved.
There are multiple types of phosphoinositide 3-kinase but only class I are responsible for lipid phosphorylation in response to growth stimuli. Class 1 PI3Ks are heterodimers composed of a regulatory subunit p85 and a catalytic subunit p110, named by their molecular weights.
The pathway can be activated by a range of signals, including hormones, growth factors and components of the extracellular matrix (ECM).[5] It is stimulated by binding of an extracellular ligand to a receptor tyrosine kinase (RTK) in the plasma membrane, causing receptor dimerization and cross-phosphorylation of tyrosine residues in the intracellular domains. The regulatory subunit p85 binds to phosphorylated tyrosine residues on the activated receptor via its Src homology 2 (SH2) domain. It then recruits the catalytic subunit p110 to form the fully active PI3K enzyme. Alternatively, adaptor molecule Grb2 binds to phospho-YXN motifs of the RTK and recruits p85 via Grb2-associated binding (GAB) scaffold protein.

[@selimrozyev5637]

Thanks author for the video. One thing to add - PDK1 phosphorylates Akt protein at Threonine (T308) residue not Tyrosine (Y).

[@rajeshbhardwaj298]

Excellent video, please note than PI3K can also be activated directly by receptor tyrosine kinase without involving Ras-GTP.

[@evamadaan4933]

I was confused in this topic and here you are with the video thank you so much👏🌟

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@hopeful3456]

Insulin receptors exist as diners and when ligands binds two diners bind to each other to form tetramer

[@piano_master_5246]

Very good video. You Indian guys are the best when explaining molecular stuff, almost all the stuff I understood via CZcams was explained by Indian youtubers. People explaining the stuff in American English somehow don't have the skill to explain stuff properly, don't know why.

[@hussainbiology]

thanks for appreciation..Glad u like my stuff

[@haiderinam1524]

thanks for the video, one thing i would change is that I would call tyrosine 308 on PKB Y306, not T308

[@sudharmabanerjee6699]

It should be Threonine (Thr or T), not Tyrosine. He was confused while explaining.

[@rabnawazkhan4312]

Goood.....waiting

[@hussainbiology]

✌️✌️

[@pritipandey112]

वेटिंग फॉर this वीडियो

[@ashishanand1163]

Your videos are simply awesome.....Please try to make more Dev. Bio . videos as CSIR NET exam is on the verge. thank you.

[@hussainbiology]

thanks Ashish for appreciation.
Glad you like it...

[@the_encephalonaire]

Sir, please tell me that can i do MSc in genetic engineering after my Bsc in microbiology ? Or what else is required to pursue MSc in genetic engineering?

[@krishnendukundu6446]

For msc biotech....u will get broad range..in industry as well as research

[@rakshithm1257]

1:29 does insulin bind RTK or insulin growth factor?

[@guptashivam6399]

Insulin as a ligand bind to rtk receptor

[@muhammadsulaiman6023]

Sir can you please tell me about the best book for such pathways????

[@hussainbiology]

Lodish Cell Biology , Alberts Cell Biology

[@likhithabb8899]

We cant write this for pi3 pathway

[@noycrf5158]

أريد شرح بالعربي بالنسبه لموضوع
PI3K-AKT

[@sudharmabanerjee6699]

Good work. Need to correct few info.

[@hussainbiology]

thanks... yes please

[@bussymed7912]

Thank you so much.
#bussymed

[@hussainbiology]

You are welcome

[@alkhader2school450]

RTk form dimers upon engagement with there ligand but the only exception lignad is insulin ??
did iam right ?

[@ikrambenali9452]

Yes

[@sheikhiqbal715]

Sir ,r u also available on telegram

[@hussainbiology]

No dear...

[@hussainbiology]

Available now

[@evamadaan4933]

@@hussainbiology @hussainbiology is this the channel

[@hussainbiology]

@@evamadaan4933 i am new to Telegram.. What i know is my id only.. and yes as Hussainbiology
1253420376

[@hussainbiology]

@@evamadaan4933 Yes Eva

[@christofferrasmussen6325]

Sorry i'm late. I am pretty certain that PDK2 does not phosphorylate S473, and that is instead mTORC2. Furthermore, you never care to explain how PDK1 and 2 are activated by PIP3

[@kennethdaniella-ab7963]

yep he did not explain it but you can read it at ncbi website, there is a ton of research paper there related to this

[@cosmicvlog8196]

Aree kehna kya chahte ho bhaiii 🙄 pura ghuma diya hindi me hi bol le

[@hussainbiology]

Hindi mai samajna hai to ye dekho
czcams.com/video/PKuGfvi27d4/video.html