Indirect and Sandwich ELISA

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[@ElnaKruger]

Just another big thank you from a biochemistry major for all your hard work! You're a talented teacher.

[@KJKP]

Sandwich ELISA starts at 7:08

[@HA-wn3gg]

you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test

[@TTHPham]

Very clear explanation and straight to the point. Thank you so much!

[@jessym7965]

no worries, I worked really hard T.T.H Pham

[@MedicoDiscurso]

I appreciate this guy so much. Saving my grade.

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The best explanations require no follow up questions. Thank you!

[@qamar8655]

OMG I cant thank you enough ♡

[@AKLECTURES]

+YulYul Addict You're welcome! :)

[@carolinadeluca7242]

Thank you from Belgium!
You are very good!
PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!

[@JustMe-zz7lw]

Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.

[@human2951]

Sir sir sir..U r saviour of a biochemist.. Profound Respect from Pakistan...

[@mirkov6850]

You are a living legend. Respect!

[@bhagyasreejm6824]

Lots of thanks .. you always boos our confidence. I always get excited to switch on your lectures

[@anupray9117]

Great!! Very helpful to an Organic Chemist developing Biopharmaceuticals... God Bless You With More Energy!!!

[@AnnaJeanine]

Thank you for this video! It must have taken a lot of effort to draw the board etc. very very much appreciated!

[@xorg1029]

Just want to say that this video is SO extremely helpful. Please post more.. you explain everything so clearly! THANK YOU!

[@sydneysimukoko]

Thanks very much Dr, My immunology course is a always easy to understand when I listen from you!

[@miriamalvarezramirez5038]

Thank you so much! I loved the way you explained all the concepts

[@JP-cp1dk]

Absolutely beautiful explanation on Sandwich ELISA! Thank you so much, this will help me with my lab reports! 🥰🥰

[@putritheresiabancin7727]

You're amazing! Thank you for making this simpler.

[@summerbreezenight]

I love your videos. They are so clearly explained. Thank you.

[@linaenger3721]

U did such a good job in explaining this method! Thank you so much for that explanation! (Y)

[@philipphill9518]

Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region.
The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells.
Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common.
I hope I didn't create any confusion. Cheers

[@ferrerajones9314]

good catch!

[@NikhilGarlapati]

Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?

[@woofox333]

saving my gpa like always
thank you so much

[@princessandbluemoon]

Thank you for uploading this! Very concise and helpful!

[@astridsiregar]

Omg im so glad i found your channel. Thank you!

[@elenaungureanu8919]

Thank you very much for this explicit lesson!

[@LAisyourgirl]

So clear and concise! Thank you!!!

[@sabasolhmirzaei8591]

Thank you so much for this clear explanation!

[@MedicSD]

That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!

[@monical2648]

the best explanation ever! thank you

[@sunnykimmy]

You are a gift. Thank you for your awesome, clear, concise and colorful explanations on these methods. :D

[@jessym7965]

no you are are gift kim.

[@Countess_Samar_SprayArt]

This video is just what i needed! thank you

[@heidihi547]

Brilliant!! Thank you. So clear.

[@tiewei1614]

Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.

[@kakossniper]

Why are you such a legend?

[@cristinavall8587]

Thank you for your help!!

[@alysia9151]

THANK YOU , THANK YOU , THANK YOU!!! GOD BLESS YOU!!

[@julianatoledo3352]

Awesome explanation!

[@kevindubrow8293]

Excellent. For medical students this is a Godsend

[@jolienmangelschots]

Very clear! I didn't understand the Sandwich Elisa really well, but now I do! Thanks!

[@meghnabose3303]

AK lectures are the best....best explanation to every topic...thank you for making such helpful videos...can't thank you enough😊

[@nevershoutnever80511]

thank you so much all the other videos I watched were so confusing but you made it so understandable!

[@okajakifragment4358]

Wow you are such a good explainer and teacher.... Keep up the good work... Kudos to your explanation

[@tsgheredagebrehiwet8467]

Excellent lecturer!

[@aihamzamar8329]

one of the best Videos

[@musiclover2636]

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i love your lectures so much thank you!!!!

[@medinlab141]

You are the best! Thank you sooooooo much

[@sohamkonkar2895]

Killed it bro! Thanks !

[@constancapages]

These videos are great! You deserve more views and likes

[@mahmoudalaa323]

you've helped me very much ,, thank you

[@mayasaba9457]

Thank you so much for this perfect explanation

[@cutielion_bunny1957]

thanks for the explanation helpd me alot !!

[@RenaissanceSTL]

Great Job!

[@wilfridewilfride5516]

Thank you very much, so well explained and understandable.

[@Radosteto0]

I finally got it right! Thanks!

[@nareshbarik5384]

Thank you ❤

[@alexaovalles9504]

So good! Thank you so much!!!!

[@elafalibrahim9828]

Very clear , thank you a lot !

[@Curious_Human954]

Sir, YOU ARE AMAZING, thank you so much! :)

[@sainat5174]

Thank you!

[@clairewolf6013]

You're seriously underrated! Thank you so much!

[@nwilczyn]

the opinion of the scientific community is that he is appropriately rated

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[@zainabalkurdi6175]

Just continue
Thank you so much

[@yewshien580]

thanksss ! very useful and detail explanation

[@rootsm3]

Great explanation now it’s clicking

[@astaganaga1906]

Thank you so much!!!!

[@Dana-rm8cs]

Wow ! This was very informative thank you

[@Silhouette-Oregano]

Thanks very much for this helpful video.

[@user-gc4wj9np4f]

thank you !

[@naveenkrishna5677]

Thanks a lot.. Very very helpful..❤

[@syimirazim4713]

Thank you so much for this very informative video :)

[@freekruka3705]

this guy rocks

[@natalieyako6613]

Amazing explanation

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thanks a lot , good explanation ! ^^

[@dyoomah17]

I spent the whole trying to understand this concept . just after I watched your video everything became clear ,thank you so much

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You are the best really🦋

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I love you. You're videos are so helpful. I wish i could just take your class

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he might not be able to wear that sexy athletic-cut v-neck in his class though, so you may be better off!!

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Thanks a lot! You are the BEST ever! I just donated:-)

[@IsraeLinoy]

Really helpful, thank you :)

[@sserene531]

Can't thank you enough

[@ronaldjamatia7119]

thank you sir. may GOD bless u

[@journeywithrahat]

Jazak ALLAH sir❤️❤️❤️

[@sakshikumari4344]

Thank you so much it helped me for exams

[@zyagimstafa]

nice explanation.....thank you soo much

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great video

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Very clear explanation ☺

[@merveyigit2931]

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[@emadnashed5992]

Great thanks

[@nohaahmed6794]

thank you

[@tihtinabirhan9214]

O my Goa! Really you are agreat lecturer!!!

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[@charitynkululeko1975]

THANK YOU!!!!!!!!!!

[@alim7532]

Excellent

[@user-jy4zj7ep1k]

Watch this on the day of your exam….I see you!
Staying up late trying to understand this
Keep going!

[@beenishkhan1590]

EXCELLENT 👍

[@paulocholaoloo2166]

Thanks alot