Lac operon
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- čas přidán 22. 06. 2016
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Overview of gene regulation in the Lac operon. Discussion of CAP, cAMP, lac repressor and allolactose in regulation of lac operon.
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This helps me understand the LAC OPERON very well without bordering reading whole Campbell 10th edition textbook.
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Thanks for this video! Great explanation! One thing to add for those who are curious is that when glucose levels are high the RNA-polymerase will bind to a promoter (not the same promoter) and transcribe a gene that codes for a repressor so that in turn can bind to the operator and stop transcription of the beta-galactosidase (LacZ) since we don't need to metabolize lactose.
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This is so much better than trying to read from the Lehninger Principles of Biochemistry textbook. I read every word about the lac operon, but did not understand any of it until this video. THANK YOU
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This was one of the best explanations of the lac operon I've heard
Especially that you explained the cAMP was very helpful. I'm german and I didn't find anything about it in german videos. Thank you!
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I would like to point out that the Operon is activated when glucose is absent and lactose is present. If glucose and lactose are present, the repressor would still remain bound
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Thank you so much for helping me understand how Lac operon works!! I didn't even understand the mechanism when my professor told us in my native language
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To sum up, when there is no glucose and lactose is present, the catabolite activator protein with high cAMP will bind to the CAP site, therefore enabling transcription(the body needs energy) while if there is glucose and lactose, the system prefers glucose over lactose and will not transcribe. The catabolite activator protein will not bind since it already has a lot of cAMP, here the lac operon acts as an regulatory sequence of gene responsible for protein synthesis. The glucose inhibits the production of cAMP(cAMP are derivatives of ATP) which makes sense since there is already a lot of energy.
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if the promoter region is located further upstream (closer to 5' cap) from the structural gene and since the RNA polymerase starts at the promoter region and moves towards the structural gene, how come we say that mRNA is transcribed in a 5' to 3' direction if it is technically reading in a 3' to 5' direction here
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